scholarly journals Development of high throughput dispersive LC--ion mobility--TOFMS techniques for analysing the human plasma proteome

2004 ◽  
Vol 3 (2) ◽  
pp. 177-186 ◽  
Author(s):  
X. Liu
Keyword(s):  
Human Plasma ◽  
High Throughput ◽  
Ion Mobility ◽  
Plasma Proteome ◽  
Human Plasma Proteome

Clinical-scale high-throughput human plasma proteome analysis: Lung adenocarcinoma

PROTEOMICS ◽  
2005 ◽  
Vol 5 (4) ◽  
pp. 1150-1159 ◽  
Author(s):  
Kiyonaga Fujii ◽  
Tomoyo Nakano ◽  
Mitsuhiro Kanazawa ◽  
Shingo Akimoto ◽  
Takashi Hirano ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Human Plasma ◽  
High Throughput ◽  
Proteome Analysis ◽  
Clinical Scale ◽  
Plasma Proteome ◽  
Human Plasma Proteome

Detectability of Plasma Proteins in SRM Measurements

2018 ◽  
Vol 16 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Olga I. Kiseleva ◽  
Elena A. Ponomarenko ◽  
Yulia A. Romashova ◽  
Ekaterina V. Poverennaya ◽  
Andrey V. Lisitsa
Keyword(s):  
Blood Plasma ◽  
Human Plasma ◽  
Limit Of Detection ◽  
Human Blood Plasma ◽  
Analytical Sensitivity ◽  
Plasma Proteome ◽  
Protein Targets ◽  
Blood Plasma Sample ◽  
Specificity And Sensitivity ◽  
Human Plasma Proteome

Background: Liquid chromatography coupled with targeted mass spectrometry underwent rapid technical evolution during last years and has become widely used technology in clinical laboratories. It offers confident specificity and sensitivity superior to those of traditional immunoassays. However, due to controversial reports on reproducibility of SRM measurements, the prospects of clinical appliance of the method are worth discussing. </P><P> Objective: The study was aimed at assessment of capabilities of SRM to achieve a thorough assembly of the human plasma proteome. </P><P> Method: We examined set of 19 human blood plasma samples to measure 100 proteins, including FDA-approved biomarkers, via SRM-assay. </P><P> Results: Out of 100 target proteins 43 proteins were confidently detected in at least two blood plasma sample runs, 36 and 21 proteins were either not detected in any run or inconsistently detected, respectively. Empiric dependences on protein detectability were derived to predict the number of biological samples required to detect with certainty a diagnostically relevant quantum of the human plasma proteome. </P><P> Conclusion: The number of samples exponentially increases with an increase in the number of protein targets, while proportionally decreasing to the logarithm of the limit of detection. Analytical sensitivity and enormous proteome heterogeneity are major bottlenecks of the human proteome exploration.

Study of the human plasma proteome of rheumatoid arthritis

2009 ◽  
Vol 1216 (16) ◽  
pp. 3538-3545 ◽  
Author(s):  
Xiaoyang Zheng ◽  
Shiaw-lin Wu ◽  
Marina Hincapie ◽  
William S. Hancock
Keyword(s):  
Rheumatoid Arthritis ◽  
Human Plasma ◽  
Plasma Proteome ◽  
Human Plasma Proteome

scholarly journals Site specific modification of the human plasma proteome by methylglyoxal

2015 ◽  
Vol 289 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Michael J. Kimzey ◽  
Owen R. Kinsky ◽  
Hussein N. Yassine ◽  
George Tsaprailis ◽  
Craig S. Stump ◽  
...  
Keyword(s):  
Human Plasma ◽  
Plasma Proteome ◽  
Site Specific ◽  
Specific Modification ◽  
Human Plasma Proteome
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