New dot-blot method for evaluating the effect of inactivators on mite and Japanese cedar pollen allergens

2021 ◽  
Vol 85 (10) ◽  
pp. 2089-2092
Author(s):  
Megumi Yoshida ◽  
Keijiro Mizukami ◽  
Keigo Kurata ◽  
Tadahiro Nasukawa ◽  
Jumpei Uchiyama ◽  
...  
Keyword(s):  
Protein Detection ◽  
Japanese Cedar ◽  
Detection Methods ◽  
Dot Blot ◽  
Pollen Allergens ◽  
Japanese Cedar Pollen ◽  
Cedar Pollen

ABSTRACT As a method of evaluating the effect of inactivators on allergens while suppressing the effect of inactivator on the assay, we developed new dot-blot method that combines immunostaining and protein detection methods. This method is useful for evaluating whether the inactivator can inactivate allergens rather than removing them from the assay.

Preclinical evaluation of an immunotherapeutic peptide comprising 7 T-cell determinants of Cry j 1 and Cry j 2, the major Japanese cedar pollen allergens

2001 ◽  
Vol 108 (1) ◽  
pp. 94-100 ◽  
Author(s):  
Kazuki Hirahara ◽  
Toru Tatsuta ◽  
Toshiro Takatori ◽  
Masahiko Ohtsuki ◽  
Hideyo Kirinaka ◽  
...  
Keyword(s):  
T Cell ◽  
Japanese Cedar ◽  
Pollen Allergens ◽  
Preclinical Evaluation ◽  
Japanese Cedar Pollen ◽  
Cedar Pollen ◽  
Cry J 2

Prevention of allergic conjunctivitis in mice by a rice-based edible vaccine containing modified Japanese cedar pollen allergens

2015 ◽  
Vol 99 (5) ◽  
pp. 705-709 ◽  
Author(s):  
Ken Fukuda ◽  
Waka Ishida ◽  
Yosuke Harada ◽  
Yuhya Wakasa ◽  
Hidenori Takagi ◽  
...  
Keyword(s):  
Allergic Conjunctivitis ◽  
Japanese Cedar ◽  
Edible Vaccine ◽  
Pollen Allergens ◽  
Japanese Cedar Pollen ◽  
Cedar Pollen

scholarly journals Seroprevalence of Immunoglobulin E Antibodies against Japanese Cedar Pollen Allergens Cry j 1 and Cry j 2 in Dogs Bred in Japan

2018 ◽  
Vol 5 (3) ◽  
pp. 79 ◽  
Author(s):  
Takashi Kuribayashi ◽  
Davide Cossu ◽  
Eiichi Momotani
Keyword(s):  
Immunoglobulin E ◽  
Japanese Cedar ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pollen Allergens ◽  
Kanagawa Prefecture ◽  
Japanese Cedar Pollen ◽  
Hyogo Prefecture ◽  
Cedar Pollen ◽  
Cry J 2

Levels of Japanese cedar pollen (Cryptomeria japonica) have increased in Japan and cedar pollinosis caused by Japanese cedar pollen has been reported in dogs. Serum levels of immunoglobulin E (IgE) against Cry j 1 and Cry j 2 in dogs raised in institutes and treated at veterinary hospitals in Japan were thus investigated. A total of 71 sera obtained from two institutes and 87 sera obtained from veterinary hospitals in the Hyogo and Kanagawa Prefectures were analyzed in this study. Serum levels of IgE were measured using the enzyme-linked immunosorbent assay with commercial purified Cry j 1 and Cry j 2. IgE against Cry j 1 and Cry j 2 in sera obtained from the two institutes were detected, despite the dogs being bred in enclosed areas. Moreover, significant differences were noted in the serum levels of IgE against Cry j 1 and Cry j 2 between the two institutes. The number of samples showing Cry j 1 or Cry j 2 levels above the cut-off values was greater in the Kanagawa Prefecture than in the Hyogo Prefecture. In total, 14 dogs showed Cry j 1 and Cry j 2 levels greater than the cut-off values in the Hyogo Prefecture, and only three such dogs were seen in the Kanagawa Prefecture. A significant correlation between serum levels against both allergens was observed (r2 = 0.6931, p < 0.0001).

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