scholarly journals Hemodynamic Characterization of a Mouse Model for Investigating the Cellular and Molecular Mechanisms of Neotissue Formation in Tissue-Engineered Heart Valves

2015 ◽  
Vol 21 (9) ◽  
pp. 987-994 ◽  
Author(s):  
Iyore A. James ◽  
Tai Yi ◽  
Shuhei Tara ◽  
Cameron A. Best ◽  
Alexander J. Stuber ◽  
...  
Keyword(s):  
Mouse Model ◽  
Heart Valves ◽  
Molecular Mechanisms ◽  
Tissue Engineered Heart Valves

Inverse Mechanical Characterization of Tissue Engineered Heart Valves

2008 ◽  
Author(s):  
Martijn A. J. Cox ◽  
Jeroen Kortsmit ◽  
Niels J. B. Driessen ◽  
Carlijn V. C. Bouten ◽  
Frank P. T. Baaijens
Keyword(s):  
Heart Valves ◽  
Soft Tissues ◽  
Mechanical Characterization ◽  
Tensile Tests ◽  
Material Behavior ◽  
Uniaxial Tensile ◽  
Mechanical Conditioning ◽  
Indentation Tests ◽  
Tissue Engineered Heart Valves

Over the last few years, research interest in tissue engineering as an alternative for current treatment and replacement strategies for cardiovascular and heart valve diseases has significantly increased. In vitro mechanical conditioning is an essential tool for engineering strong implantable tissues [1]. Detailed knowledge of the mechanical properties of the native tissue as well as the properties of the developing engineered constructs is vital for a better understanding and control of the mechanical conditioning process. The nonlinear and anisotropic behavior of soft tissues puts high demands on their mechanical characterization. Current standards in mechanical testing of soft tissues include (multiaxial) tensile testing and indentation tests. Uniaxial tensile tests do not provide sufficient information for characterizing the full anisotropic material behavior, while biaxial tensile tests are difficult to perform, and boundary effects limit the test region to a small central portion of the tissue. In addition, characterization of the local tissue properties from a tensile test is non-trivial. Indentation tests may be used to overcome some of these limitations. Indentation tests are easy to perform and when indenter size is small relative to the tissue dimensions, local characterization is possible. We have demonstrated that by recording deformation gradients and indentation force during a spherical indentation test the anisotropic mechanical behavior of engineered cardiovascular constructs can be characterized [2]. In the current study this combined numerical-experimental approach is used on Tissue Engineered Heart Valves (TEHV).

scholarly journals Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves

2014 ◽  
Vol 1 (2) ◽  
pp. 146-162 ◽  
Author(s):  
Monica Fahrenholtz ◽  
Huiwen Liu ◽  
Debra Kearney ◽  
Lalita Wadhwa ◽  
Charles Fraser ◽  
...  
Keyword(s):  
Heart Valves ◽  
Dermal Fibroblasts ◽  
Cell Source ◽  
A Cell ◽  
Tissue Engineered Heart Valves

In Vivo Remodeling and Structural Characterization of Fibrin-Based Tissue-Engineered Heart Valves in the Adult Sheep Model

2009 ◽  
Vol 15 (10) ◽  
pp. 2965-2976 ◽  
Author(s):  
Thomas C. Flanagan ◽  
Jörg S. Sachweh ◽  
Julia Frese ◽  
Heike Schnöring ◽  
Nina Gronloh ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Heart Valves ◽  
Sheep Model ◽  
Adult Sheep ◽  
Tissue Engineered Heart Valves

scholarly journals Flexural characterization of cell encapsulated PEGDA hydrogels with applications for tissue engineered heart valves

2011 ◽  
Vol 7 (6) ◽  
pp. 2467-2476 ◽  
Author(s):  
Christopher A. Durst ◽  
Michael P. Cuchiara ◽  
Elizabeth G. Mansfield ◽  
Jennifer L. West ◽  
K. Jane Grande-Allen
Keyword(s):  
Heart Valves ◽  
Tissue Engineered Heart Valves

scholarly journals Attenuated Signature-Tagged Mutagenesis Mutants ofBrucella melitensis Identified during the Acute Phase ofInfection inMice

2003 ◽  
Vol 71 (12) ◽  
pp. 7053-7060 ◽  
Author(s):  
P. Lestrate ◽  
A. Dricot ◽  
R.-M. Delrue ◽  
C. Lambert ◽  
V. Martinelli ◽  
...  
Keyword(s):  
Mouse Model ◽  
Molecular Mechanisms ◽  
Brucella Melitensis ◽  
Functional Protein ◽  
Transposon Insertion ◽  
Gene Coding ◽  
Insertion Sites ◽  
Mouse Model Of Infection

ABSTRACT For this study, we screened 1,152 signature-tagged mutagenesis mutants of Brucella melitensis 16M in a mouse model of infection and found 36 of them to be attenuated in vivo. Molecular characterization of transposon insertion sites showed that for four mutants, the affected genes were only present in Rhizobiaceae. Another mutant contained a disruption in a gene homologous to mosA, which is involved in rhizopine biosynthesis in some strains of Rhizobium, suggesting that this sugar may be involved in Brucella pathogenicity. A mutant was disrupted in a gene homologous to fliF, a gene potentially coding for the MS ring, a basal component of the flagellar system. Surprisingly, a mutant was affected in the rpoA gene, coding for the essentialα -subunit of the RNA polymerase. This disruption leaves a partially functional protein, impaired for the activation of virB transcription, as demonstrated by the absence of induction of the virB promoter in the Tn5::rpoA background. The results presented here highlight the fact that the ability of Brucella to induce pathogenesis shares similarities with the molecular mechanisms used by both Rhizobium and Agrobacterium to colonize their hosts.

In vivo repopulation of tissue engineered heart valves

2010 ◽  
Vol 58 (S 01) ◽  
Author(s):  
PM Dohmen ◽  
A Lembcke ◽  
S Holinski ◽  
JP Braun ◽  
W Konertz
Keyword(s):  
Heart Valves ◽  
Tissue Engineered Heart Valves
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