scholarly journals Characterization of Dermal Fibroblasts as a Cell Source for Pediatric Tissue Engineered Heart Valves

2014 ◽  
Vol 1 (2) ◽  
pp. 146-162 ◽  
Author(s):  
Monica Fahrenholtz ◽  
Huiwen Liu ◽  
Debra Kearney ◽  
Lalita Wadhwa ◽  
Charles Fraser ◽  
...  
Keyword(s):  
Heart Valves ◽  
Dermal Fibroblasts ◽  
Cell Source ◽  
A Cell ◽  
Tissue Engineered Heart Valves

Abstract 219: Induced Pluripotent Stem Cells as a Potential Cell Source for Tissue Engineered Heart Valves

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Aline L Yonezawa ◽  
Monalisa Singh ◽  
David Safranski ◽  
Kenneth M Dupont ◽  
Chunhui Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Heart Valves ◽  
3D Culture ◽  
Polyethylene Glycol Diacrylate ◽  
Glycol Diacrylate ◽  
Cell Source ◽  
Induced Pluripotent ◽  
Tissue Engineered Heart Valves

Despite recent advances in tissue engineered heart valves (TEHV), one of the major challenges is finding a suitable cell source for seeding TEHV scaffolds. Native heart valves are durable because valve interstitial cells (VICs) maintain tissue homeostasis by synthesizing and remodeling the extracellular matrix. In this study, we demonstrate that induced pluripotent stem cells (iPSCs) can be derived into induced mesenchymal stem cells (iMSCs) using our feeder-free protocol and then further differentiated into VICs using a 3D cell culture environment. The differentiation efficiency was quantified using flow cytometry, immunohistochemistry staining, RT-PCR, and trilineage differentiation. In addition, iMSCs were encapsulated in polyethylene (glycol) diacrylate (PEGDA) hydrogels of varying stiffness, grafted with adhesion peptide (RGDS), to promote cell proliferation, remodeling, and further differentiation into VIC-like cells. VICs phenotype was characterized by the expression of αSMA, vimentin, F-actin, and the ECM production after 7, 14, and 21 days. The results demonstrated that using our feeder-free differentiation protocol, iMSCs were differentiated from iPSCs. Our iMSCs had a 99.9% and 99.4% positive expression for MSC markers CD90 and CD44, respectively. As expected, there was 0.019% expression of CD45, which is a hematopoietic marker. In addition, iMSCs differentiated into adipogenic, chondrogenic, and osteogenic. When MSC derived cells were encapsulated in PEGDA hydrogels that mimic the leaflet modulus, we observed expression of αSMA and F-actin after 7 days. Thus, the results from this study suggest that iPSCs can be a suitable cell source for TEHV by using a feeder-free differentiation approach and 3D culture.

Inverse Mechanical Characterization of Tissue Engineered Heart Valves

2008 ◽  
Author(s):  
Martijn A. J. Cox ◽  
Jeroen Kortsmit ◽  
Niels J. B. Driessen ◽  
Carlijn V. C. Bouten ◽  
Frank P. T. Baaijens
Keyword(s):  
Heart Valves ◽  
Soft Tissues ◽  
Mechanical Characterization ◽  
Tensile Tests ◽  
Material Behavior ◽  
Uniaxial Tensile ◽  
Mechanical Conditioning ◽  
Indentation Tests ◽  
Tissue Engineered Heart Valves

Over the last few years, research interest in tissue engineering as an alternative for current treatment and replacement strategies for cardiovascular and heart valve diseases has significantly increased. In vitro mechanical conditioning is an essential tool for engineering strong implantable tissues [1]. Detailed knowledge of the mechanical properties of the native tissue as well as the properties of the developing engineered constructs is vital for a better understanding and control of the mechanical conditioning process. The nonlinear and anisotropic behavior of soft tissues puts high demands on their mechanical characterization. Current standards in mechanical testing of soft tissues include (multiaxial) tensile testing and indentation tests. Uniaxial tensile tests do not provide sufficient information for characterizing the full anisotropic material behavior, while biaxial tensile tests are difficult to perform, and boundary effects limit the test region to a small central portion of the tissue. In addition, characterization of the local tissue properties from a tensile test is non-trivial. Indentation tests may be used to overcome some of these limitations. Indentation tests are easy to perform and when indenter size is small relative to the tissue dimensions, local characterization is possible. We have demonstrated that by recording deformation gradients and indentation force during a spherical indentation test the anisotropic mechanical behavior of engineered cardiovascular constructs can be characterized [2]. In the current study this combined numerical-experimental approach is used on Tissue Engineered Heart Valves (TEHV).

Sufficient Tissue Engineered Heart Valves: A Question of Cell Source?

2012 ◽  
Vol 2012 (4) ◽  
pp. 33
Author(s):  
Miriam Weber ◽  
Julia Frese ◽  
Nima Hatam ◽  
Joerg Sachweh ◽  
Thomas Schmitz-Rode ◽  
...  
Keyword(s):  
Heart Valves ◽  
Cell Source ◽  
Tissue Engineered Heart Valves

Bone marrow as a cell source for tissue engineering heart valves

2003 ◽  
Vol 75 (3) ◽  
pp. 761-767 ◽  
Author(s):  
Tjörvi E Perry ◽  
Sunjay Kaushal ◽  
Fraser W.H Sutherland ◽  
Kristine J Guleserian ◽  
Joyce Bischoff ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Heart Valves ◽  
Cell Source ◽  
A Cell

In Vivo Remodeling and Structural Characterization of Fibrin-Based Tissue-Engineered Heart Valves in the Adult Sheep Model

2009 ◽  
Vol 15 (10) ◽  
pp. 2965-2976 ◽  
Author(s):  
Thomas C. Flanagan ◽  
Jörg S. Sachweh ◽  
Julia Frese ◽  
Heike Schnöring ◽  
Nina Gronloh ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Heart Valves ◽  
Sheep Model ◽  
Adult Sheep ◽  
Tissue Engineered Heart Valves

Controlled Cyclic Stretching of Tissue Engineered Heart Valves: Effects on Mechanical Properties and ECM Organization

2008 ◽  
Author(s):  
Zeeshan H. Syedain ◽  
Robert T. Tranquillo
Keyword(s):  
Mechanical Properties ◽  
Strain Amplitude ◽  
Heart Valves ◽  
Dermal Fibroblasts ◽  
Fibrin Gels ◽  
Cyclic Stretching ◽  
Tissue Constructs ◽  
Cell Sources ◽  
Tissue Engineered Heart Valves

Tissue engineering provides a means to create fully functional tissue-equivalents that can grow, repair and remodel in vivo. Our laboratory’s approach to fabricating artery- and heart valve-equivalents utilizes cell-seeded fibrin gels. However, even after 4–5 weeks of static incubation, the mechanical properties of these constructs are below those of native tissue. Previous studies in our laboratory have shown a significant role of mechanical stretching in improving properties of collagen-based tissue constructs (Isenberg and Tranquillo, 2003). We examined the effects of cyclic distention (CD) of cell-seeded fibrin-based tubular constructs (TC) and valve-equivalents (VE) after five weeks of culture. We used human dermal fibroblasts and porcine valve interstitial cells as the cell sources. Circumferential strain amplitudes from 2.5% to 15% were applied to evaluate the effects of CD on remodeling of the TC. We further hypothesized that during long-term conditioning, cells adapt to CD of constant strain amplitude, diminishing the remodeling into tissue. We tested this hypothesis by applying step-wise incremental CD (ICD) from 5%–15% strain amplitude and compared this group to a set of samples subject to CD of constant strain amplitude in this range. Based on the outcome of the cyclic distension study with tubular constructs, we applied CD to VE in a novel bioreactor.

The influence of the cell source on the sufficiency of tissue engineered heart valves

2012 ◽  
Vol 60 (S 01) ◽  
Author(s):  
M Weber ◽  
J Frese ◽  
T Schmitz-Rode ◽  
S Jockenhoevel ◽  
P Mela
Keyword(s):  
Heart Valves ◽  
Cell Source ◽  
Tissue Engineered Heart Valves

scholarly journals Flexural characterization of cell encapsulated PEGDA hydrogels with applications for tissue engineered heart valves

2011 ◽  
Vol 7 (6) ◽  
pp. 2467-2476 ◽  
Author(s):  
Christopher A. Durst ◽  
Michael P. Cuchiara ◽  
Elizabeth G. Mansfield ◽  
Jennifer L. West ◽  
K. Jane Grande-Allen
Keyword(s):  
Heart Valves ◽  
Tissue Engineered Heart Valves

Novel Tissue-Engineered Heart Valve

2013 ◽  
Author(s):  
Zeeshan Syedain ◽  
Lee Meier ◽  
Jay Reimer ◽  
Robert Tranquillo
Keyword(s):  
Heart Valve ◽  
Heart Valves ◽  
Pediatric Patients ◽  
Dermal Fibroblasts ◽  
Pulse Flow ◽  
Novel Approach ◽  
Fibrin Scaffold ◽  
Tissue Engineered Heart Valve ◽  
Tissue Engineered Heart Valves

Tissue-engineered heart valves (TEHV) have the potential to revolutionize valve replacements therapies, especially for pediatric patients. While much progress has been made toward implanting a TEHV, a major limitation to date has been in vivo leaflet retraction due to the contractile nature of the cells transplanted within the TEHV. This phenomenon has been problematic in numerous studies, particularly for approaches employing the use of a fibrin scaffold (Syedain et al. 2011, Flanagan et al. 2009). Additional challenges in the development of a TEHV include designing a 3D mold that allows for proper coaptation and functionality of engineered leaflets. Herein, we present a novel approach for developing a TEHV from a decellularized engineered tube fabricated from fibrin that is remodeled by entrapped dermal fibroblasts, and matured using a custom pulse flow-stretch bioreactor. This approach has the potential to deliver an off-the-shelf engineered heart valve that exhibits the ability to be readily recellularized in contrast to current clinically employed tissue-based valve replacements.

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