scholarly journals Quantitative Validation of the Presto Blue™ Metabolic Assay for Online Monitoring of Cell Proliferation in a 3D Perfusion Bioreactor System

2015 ◽  
Vol 21 (6) ◽  
pp. 519-529 ◽  
Author(s):  
Maarten Sonnaert ◽  
Ioannis Papantoniou ◽  
Frank P. Luyten ◽  
Jan Schrooten
Keyword(s):  
Cell Proliferation ◽  
Online Monitoring ◽  
Perfusion Bioreactor ◽  
Quantitative Validation ◽  
Bioreactor System

Development of a Perfusion Bioreactor System for Alveolar Bone Tissue Engineering

2009 ◽  
Author(s):  
Ki Taek Lim ◽  
Pill Hoon Choung ◽  
Jang Ho Kim ◽  
Hyun Mok Son ◽  
Hoon Seonwoo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Alveolar Bone ◽  
Perfusion Bioreactor ◽  
Bioreactor System

scholarly journals Cultivation of hierarchical 3D scaffolds inside a perfusion bioreactor: scaffold design and finite-element analysis of fluid flow

2021 ◽  
Vol 3 (12) ◽  
Author(s):  
Kaylie Sampson ◽  
Songmi Koo ◽  
Carter Gadola ◽  
Anastasiia Vasiukhina ◽  
Aditya Singh ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Perfusion Bioreactor ◽  
Osteoporotic Bone ◽  
Scaffold Design ◽  
Culture Dish ◽  
Element Analysis ◽  
3D Scaffolds ◽  
Tissue Culture Dish ◽  
Thermally Induced ◽  
Bone Nonunion

AbstractThe use of porous 3D scaffolds for the repair of bone nonunion and osteoporotic bone is currently an area of great interest. Using a combination of thermally-induced phase separation (TIPS) and 3D-plotting (3DP), we have generated hierarchical 3DP/TIPS scaffolds made of poly(lactic-co-glycolic acid) (PLGA) and nanohydroxyapatite (nHA). A full factorial design of experiments was conducted, in which the PLGA and nHA compositions were varied between 6‒12% w/v and 10‒40% w/w, respectively, totaling 16 scaffold formulations with an overall porosity ranging between 87%‒93%. These formulations included an optimal scaffold design identified in our previous study. The internal structures of the scaffolds were examined using scanning electron microscopy and microcomputed tomography. Our optimal scaffold was seeded with MC3T3-E1 murine preosteoblastic cells and subjected to cell culture inside a tissue culture dish and a perfusion bioreactor. The results were compared to those of a commercial CellCeram™ scaffold with a composition of 40% β-tricalcium phosphate and 60% hydroxyapatite (β-TCP/HA). Media flow within the macrochannels of 3DP/TIPS scaffolds was modeled in COMSOL software in order to fine tune the wall shear stress. CyQUANT DNA assay was performed to assess cell proliferation. The normalized number of cells for the optimal scaffold was more than twofold that of CellCeram™ scaffold after two weeks of culture inside the bioreactor. Despite the substantial variability in the results, the observed improvement in cell proliferation upon culture inside the perfusion bioreactor (vs. static culture) demonstrated the role of macrochannels in making the 3DP/TIPS scaffolds a promising candidate for scaffold-based tissue engineering.

Using 3D perfusion bioreactor system to studying cell biology of ovarian cancer.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e17558-e17558
Author(s):  
Alba Martínez ◽  
Molly Buckley ◽  
Joel Berry ◽  
Rebecca Christian Arend
Keyword(s):  
Ovarian Cancer ◽  
Cell Culture ◽  
Cell Growth ◽  
Cell Lines ◽  
Culture Media ◽  
Tumor Biology ◽  
Perfusion Bioreactor ◽  
Suitable Model ◽  
Cell Culture Media ◽  
Bioreactor System

e17558 Background: Epithelial Ovarian Cancer (EOC) is the most common cause of death among gynecological malignancies. This is a result of the high rate of recurrence and chemo-resistance in EOC patients. Therefore, the development of new therapeutics is crucial. A major factor contributing to this is the lack of therapeutic candidates is lack of translational accuracy in preclinical models. Recently, 3-dimensional (3-D) models have aided in accurately recreating tumor biology. We have developed an EOC 3-D perfused bioreactor system that recapitulates EOC tumor biology and incorporates tumor biomechanical regulation. This model allows for us to more accurately predict the clinical response of new drug candidates, which aids in elimination of ineffective candidates prior to clinical trials. Methods: EOC cell lines (luciferase-taggedSKOV-3 and OVCAR-8) were embedded in a relevant extracellular matrix (ECM) and injected into a perfused, polydimethylsiloxane (PDMS) bioreactor. Microchannels were embedded in matrigel so that the cell culture media with or without chemotherapy could flow through the perfused PDMS to provide nutrient delivery and gas exchange enhancing viability and function of surrounding cells. The bioreactors were connected to a peristaltic pump that allowed for the cell culture media to perfuse over a 7-day period. We monitored cell viability using bioluminescence imaging (BLI), immunohistochemistry (IHC), and lactate dehydrogenase (LDH) release in media. Results: BLI showed a linear increase in SKOV-3 and OVCAR-8 cell growth over 7 days. These results were confirmed by IHC measuring the number of nucleated cells per micron2. Graphical representation of the region of interest (ROI) showed a high correlation between IHC staining of nucleated cells and BLI score. IHC analysis of PAX8 staining was positive and proved that the perfusion bioreactor system maintains EOC biology over time. In addition, our results suggest that the bioreactor is a suitable model for drug preclinical testing in both cell lines as well as in patients’ samples. Conclusions: Our preliminary results using the 3D EOC perfused, PDMS bioreactor model showed increased EOC cell growth overtime, while maintaining original EOC histology. Moreover, our results suggest that this model could provide a novel platform to study therapeutic interventions in EOC. Our ultimate goal is to implement ovarian cancer microenvironment components (e.g. immune cells) into bioreactor system to study different drug treatments to better determine drug candidate’s translational efficacy.

Pulsatile Perfusion Bioreactor System for Durability Testing and Compliance Estimation of Tissue Engineered Vascular Grafts

2013 ◽  
Vol 41 (9) ◽  
pp. 1979-1989 ◽  
Author(s):  
Stefanos E. Diamantouros ◽  
Luis G. Hurtado-Aguilar ◽  
Thomas Schmitz-Rode ◽  
Petra Mela ◽  
Stefan Jockenhoevel
Keyword(s):  
Vascular Grafts ◽  
Perfusion Bioreactor ◽  
Durability Testing ◽  
Bioreactor System ◽  
Pulsatile Perfusion

scholarly journals A perfusion bioreactor system efficiently generates cell‐loaded bone substitute materials for addressing critical size bone defects

2015 ◽  
Vol 10 (11) ◽  
pp. 1727-1738 ◽  
Author(s):  
Claudia Kleinhans ◽  
Ramkumar Ramani Mohan ◽  
Gabriele Vacun ◽  
Thomas Schwarz ◽  
Barbara Haller ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Critical Size ◽  
Bone Defects ◽  
Perfusion Bioreactor ◽  
Bone Substitute Materials ◽  
Bioreactor System ◽  
Substitute Materials

scholarly journals A Perfusion Bioreactor System for Cell Seeding and Oxygen-Controlled Cultivation of Three-Dimensional Cell Cultures

2018 ◽  
Vol 24 (10) ◽  
pp. 585-595 ◽  
Author(s):  
Jakob Schmid ◽  
Sascha Schwarz ◽  
Robert Meier-Staude ◽  
Stefanie Sudhop ◽  
Hauke Clausen-Schaumann ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Three Dimensional ◽  
Perfusion Bioreactor ◽  
Cell Seeding ◽  
Bioreactor System ◽  
Dimensional Cell

scholarly journals Validation of a novel perfusion bioreactor system in cancer research

2020 ◽  
Vol 74 (3) ◽  
pp. 187-196
Author(s):  
Jasmina Stojkovska ◽  
Jovana Zvicer ◽  
Milena Milivojevic ◽  
Isidora Petrovic ◽  
Milena Stevanovic ◽  
...  
Keyword(s):  
Cancer Research ◽  
Drug Screening ◽  
Cancer Drug ◽  
Perfusion Bioreactor ◽  
Animal Testing ◽  
Anti Cancer ◽  
Medium Flow ◽  
Bioreactor System

Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100?250 ?m s-1) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.

scholarly journals Enhanced extracellular vesicle production and ethanol-mediated vascularization bioactivity via a 3D-printed scaffold-perfusion bioreactor system

2019 ◽  
Vol 95 ◽  
pp. 236-244 ◽  
Author(s):  
Divya B. Patel ◽  
Christopher R. Luthers ◽  
Max J. Lerman ◽  
John P. Fisher ◽  
Steven M. Jay
Keyword(s):  
Extracellular Vesicle ◽  
Perfusion Bioreactor ◽  
3D Printed ◽  
Bioreactor System
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