Development of a Perfusion Bioreactor System for Alveolar Bone Tissue Engineering

2009 ◽  
Author(s):  
Ki Taek Lim ◽  
Pill Hoon Choung ◽  
Jang Ho Kim ◽  
Hyun Mok Son ◽  
Hoon Seonwoo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Alveolar Bone ◽  
Perfusion Bioreactor ◽  
Bioreactor System

A clinical study of alveolar bone tissue engineering with cultured autogenous periosteal cells: Coordinated activation of bone formation and resorption

Bone ◽  
2012 ◽  
Vol 50 (5) ◽  
pp. 1123-1129 ◽  
Author(s):  
Masaki Nagata ◽  
Hideyuki Hoshina ◽  
Minqi Li ◽  
Megumi Arasawa ◽  
Kohya Uematsu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Clinical Study ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Alveolar Bone ◽  
Periosteal Cells

scholarly journals Clinical Outcome and 8-Year Follow-Up of Alveolar Bone Tissue Engineering for Severely Atrophic Alveolar Bone Using Autologous Bone Marrow Stromal Cells with Platelet-Rich Plasma and β-Tricalcium Phosphate Granules

2021 ◽  
Vol 10 (22) ◽  
pp. 5231
Author(s):  
Izumi Asahina ◽  
Hideaki Kagami ◽  
Hideki Agata ◽  
Masaki J. Honda ◽  
Yoshinori Sumita ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Marrow Stromal Cells ◽  
Alveolar Bone ◽  
Platelet Rich Plasma ◽  
Long Term Follow Up

Background: Although bone tissue engineering for dentistry has been studied for many years, the clinical outcome for severe cases has not been established. Furthermore, there are limited numbers of studies that include long-term follow-up. In this study, the safety and efficacy of bone tissue engineering for patients with a severely atrophic alveolar bone were examined using autogenous bone marrow stromal cells (BMSCs), and the long-term stability was also evaluated. Methods: BMSCs from iliac bone marrow aspirate were cultured and expanded. Then, induced osteogenic cells were transplanted with autogenous platelet-rich plasma (PRP) and β-tricalcium phosphate granules (β-TCP) for maxillary sinus floor and alveolar ridge augmentation. Eight patients (two males and six females) with an average age of 54.2 years underwent cell transplantation. Safety was assessed by monitoring adverse events. Radiographic evaluation and bone biopsies were performed to evaluate the regenerated bone. Results: The major population of transplanted BMSCs belonged to the fraction of CD34−, CD45dim, and CD73+ cells, which was only 0.065% of the total bone marrow cells. Significant deviations were observed in cell growth and alkaline phosphatase activities among individuals. However, bone regeneration was observed in all patients and the average bone area in the biopsy samples was 41.9% 6 months following transplantation, although there were also significant deviations among each case. No adverse events related to the transplants were observed. In the regenerated bone, 27 out of 29 dental implants were integrated. Dental implants and regenerated bone were stable for an average follow-up period of 7 years and 10 months. Conclusions: Although individual variations were observed, the results showed that bone tissue engineering using BMSCs with PRP and β-TCP was feasible for patients with severe atrophic maxilla throughout a long-term follow-up period and was considered safe. However, further studies with a larger number of cases and controls to confirm the efficacy of BMSCs and the development of a protocol to establish a reproducible quality of stem cell-based graft material will be required.

Computational modeling of media flow through perfusion-based bioreactors for bone tissue engineering

2020 ◽  
Vol 234 (12) ◽  
pp. 1397-1408
Author(s):  
Hanieh Nokhbatolfoghahaei ◽  
Mahboubeh Bohlouli ◽  
Kazem Adavi ◽  
Zahrasadat Paknejad ◽  
Maryam Rezai Rad ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Shear Stress ◽  
Fluid Flow ◽  
Computational Modeling ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Fluid Motion ◽  
Shear Loading ◽  
Perfusion Bioreactor ◽  
Perfusion Bioreactors

Bioreactor system has been used in bone tissue engineering in order to simulate dynamic nature of bone tissue environments. Perfusion bioreactors have been reported as the most efficient types of shear-loading bioreactor. Also, combination of forces, such as rotation plus perfusion, has been reported to enhance cell growth and osteogenic differentiation. Mathematical modeling using sophisticated infrastructure processes could be helpful and streamline the development of functional grafts by estimating and defining an effective range of bioreactor settings for better augmentation of tissue engineering. This study is aimed to conduct computational modeling for newly designed bioreactors in order to alleviate the time and material consuming for evaluating bioreactor parameters and effect of fluid flow hydrodynamics (various amounts of shear stress) on osteogenesis. Also, biological assessments were performed in order to validate similar parameters under implementing the perfusion or rotating and perfusion fluid motions in bioreactors’ prototype. Finite element method was used to investigate the effect of hydrodynamic of fluid flow inside the bioreactors. The equations used in the simulation to calculate the velocity values and consequently the shear stress values include Navier–Stokes and Brinkman equations. It has been shown that rotational fluid motion in rotating and perfusion bioreactor produces more velocity and shear stress compared with perfusion bioreactor. Moreover, implementing the perfusion together with rotational force in rotating and perfusion bioreactors has been shown to have more cell proliferation and higher activity of alkaline phosphatase enzyme as well as formation of extra cellular matrix sheet, as an indicator of bone-like tissue formation.

Design and Assessment of a Dynamic Perfusion Bioreactor for Large Bone Tissue Engineering Scaffolds

2017 ◽  
Vol 185 (2) ◽  
pp. 555-563 ◽  
Author(s):  
Birru Bhaskar ◽  
Robert Owen ◽  
Hossein Bahmaee ◽  
Parcha Sreenivasa Rao ◽  
Gwendolen C. Reilly
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Perfusion Bioreactor ◽  
Tissue Engineering Scaffolds ◽  
Large Bone
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