scholarly journals A perfusion bioreactor system efficiently generates cell‐loaded bone substitute materials for addressing critical size bone defects

2015 ◽  
Vol 10 (11) ◽  
pp. 1727-1738 ◽  
Author(s):  
Claudia Kleinhans ◽  
Ramkumar Ramani Mohan ◽  
Gabriele Vacun ◽  
Thomas Schwarz ◽  
Barbara Haller ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Critical Size ◽  
Bone Defects ◽  
Perfusion Bioreactor ◽  
Bone Substitute Materials ◽  
Bioreactor System ◽  
Substitute Materials

Effect of Silicon-Doped Calcium Phosphate Bone Substitutes on Bone Formation and Osteoblastic Phenotype Expression In Vivo

2014 ◽  
Vol 614 ◽  
pp. 31-34 ◽  
Author(s):  
Christine Knabe ◽  
Marco Lopez Heredia ◽  
Dirk Barnemitz ◽  
Antje Genzel ◽  
Fabian Peters ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Substitute ◽  
Bone Repair ◽  
Critical Size ◽  
Potassium Phosphate ◽  
Critical Size Defects ◽  
Bone Substitute Materials ◽  
Silicon Doped ◽  
Substitute Materials

This study evaluates the effect of two novel particulate silicon-doped calcium phosphate graft materials as compared to the currently clinically used material β-TCP on osteogenesis and bone formation after implantation in critical-size defects the sheep scapula. These materials were developed in order to create biodegradable bone substitute materials that degrade rapidly, but still stimulate osteogenesis at the same time, thereby resulting in bone repair and regeneration with fully functional bone tissue. All bone substitute materials studied facilitated excellent bony regeneration of critical-size defects in the sheep scapula. Of the three grafting materials studied, the calcium alkali orthophosphate material with the crystalline phase Ca2KNa (PO4)2, with a small amorphous portion containing magnesium potassium phosphate and a small addition of sodium magnesium silicate had the greatest stimulatory effect on bone formation and expression of osteogenic markers, while exhibiting the highest biodegradability.

scholarly journals Pre-Clinical Evaluation of Biological Bone Substitute Materials for Application in Highly Loaded Skeletal Sites

Biomolecules ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 883 ◽  
Author(s):  
Sónia de Lacerda Schickert ◽  
Jeroen J.J.P. van den Beucken ◽  
Sander C.G. Leeuwenburgh ◽  
John A. Jansen
Keyword(s):  
Animal Models ◽  
Bone Substitute ◽  
Mechanical Stability ◽  
Ex Vivo ◽  
Bone Defects ◽  
Comprehensive Overview ◽  
Bone Defect Healing ◽  
Bone Substitute Materials ◽  
Substitute Materials ◽  
Highly Loaded

The development of bone substitute materials (BSMs) intended for load-bearing bone defects is highly complicated, as biological and mechanical requirements are often contradictory. In recent years, biological BSMs have been developed which allow for a more efficient integration of the material with the surrounding osseous environment and, hence, a higher mechanical stability of the treated defect. However, while these materials are promising, they are still far from ideal. Consequently, extensive preclinical experimentation is still required. The current review provides a comprehensive overview of biomechanical considerations relevant for the design of biological BSMs. Further, the preclinical evaluation of biological BSMs intended for application in highly loaded skeletal sites is discussed. The selected animal models and implantation site should mimic the pathophysiology and biomechanical loading patterns of human bone as closely as possible. In general, sheep are among the most frequently selected animal models for the evaluation of biomaterials intended for highly loaded skeletal sites. Regarding the anatomical sites, segmental bone defects created in the limbs and spinal column are suggested as the most suitable. Furthermore, the outcome measurements used to assess biological BSMs for regeneration of defects in heavily loaded bone should be relevant and straightforward. The quantitative evaluation of bone defect healing through ex vivo biomechanical tests is a valuable addition to conventional in vivo tests, as it determines the functional efficacy of BSM-induced bone healing. Finally, we conclude that further standardization of preclinical studies is essential for reliable evaluation of biological BSMs in highly loaded skeletal sites.

Using fractal dimension to evaluate alveolar bone defects treated with various bone substitute materials

Open Medicine ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. 776-789 ◽  
Author(s):  
Marcin Kozakiewicz ◽  
Sławomir Chaberek ◽  
Katarzyna Bogusiak
Keyword(s):  
Fractal Dimension ◽  
Bone Substitute ◽  
Alveolar Bone ◽  
Bone Defects ◽  
Bone Substitute Materials ◽  
Substitute Materials

Abstract

scholarly journals Spongostan™ Leads to Increased Regeneration of a Rat Calvarial Critical Size Defect Compared to NanoBone® and Actifuse

Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 1961
Author(s):  
Dirk Wähnert ◽  
Julian Koettnitz ◽  
Madlen Merten ◽  
Daniel Kronenberg ◽  
Richard Stange ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Critical Size ◽  
Maxillofacial Surgery ◽  
Bone Volume ◽  
Oral And Maxillofacial Surgery ◽  
Mineral Density ◽  
Critical Size Defect ◽  
Topological Features ◽  
Bone Substitute Materials ◽  
Substitute Materials

Bone substitute materials are becoming increasingly important in oral and maxillofacial surgery. Reconstruction of critical size bone defects is still challenging for surgeons. Here, we compared the clinically applied organic bone substitute materials NanoBone® (nanocrystalline hydroxyapatite and nanostructured silica gel; n = 5) and Actifuse (calcium phosphate with silicate substitution; n = 5) with natural collagen-based Spongostan™ (hardened pork gelatin containing formalin and lauryl alcohol; n = 5) in bilateral rat critical-size defects (5 mm diameter). On topological level, NanoBone is known to harbour nanopores of about 20 nm diameter, while Actifuse comprises micropores of 200–500 µm. Spongostan™, which is clinically applied as a haemostatic agent, combines in its wet form both nano- and microporous topological features by comprising 60.66 ± 24.48 μm micropores accompanied by nanopores of 32.97 ± 1.41 nm diameter. Micro-computed tomography (µCT) used for evaluation 30 days after surgery revealed a significant increase in bone volume by all three bone substitute materials in comparison to the untreated controls. Clearly visual was the closure of trepanation in all treated groups, but granular appearance of NanoBone® and Actifuse with less closure at the margins of the burr holes. In contrast, transplantion of Spongostan™ lead to complete filling of the burr hole with the highest bone volume of 7.98 ccm and the highest bone mineral density compared to all other groups. In summary, transplantation of Spongostan™ resulted in increased regeneration of a rat calvarial critical size defect compared to NanoBone and Actifuse, suggesting the distinct nano- and microtopography of wet Spongostan™ to account for this superior regenerative capacity. Since Spongostan™ is a clinically approved product used primarily for haemostasis, it may represent an interesting alternative in the reconstruction of defects in the maxillary region.

Bone Substitute Materials in Modern Dentistry

2017 ◽  
Vol 1 (4) ◽  
Author(s):  
Andrius Geguzis ◽  
Inesa Astramskaite ◽  
Dovile Gabseviciute
Keyword(s):  
Bone Substitute ◽  
Bone Substitute Materials ◽  
Substitute Materials

In vitro effects of particulate bone substitute materials on the resorption activity of human osteoclasts

2017 ◽  
Vol 34 ◽  
pp. 291-306 ◽  
Author(s):  
G Russmueller ◽  
◽  
L Winkler ◽  
R Lieber ◽  
R Seemann ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Bone Substitute Materials ◽  
In Vitro Effects ◽  
Substitute Materials ◽  
Human Osteoclasts
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