Anti-Infection Tissue Engineering Construct Treating Osteomyelitis in Rabbit Tibia

2013 ◽  
Vol 19 (1-2) ◽  
pp. 255-263 ◽  
Author(s):  
Junchao Xing ◽  
Tianyong Hou ◽  
Bianba Luobu ◽  
Fei Luo ◽  
Qianbo Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Rabbit Tibia ◽  
Tissue Engineering Construct

Tissue Engineering Construct on the Basis of Multipotent Stromal Adipose Tissue Cells and Osteomatrix for Regeneration of the Bone Tissue

2011 ◽  
Vol 152 (1) ◽  
pp. 153-158 ◽  
Author(s):  
T. B. Bukharova ◽  
I. V.Arutyunyan ◽  
S. A. Shustrov ◽  
I. S. Alekseeva ◽  
I. A. Fedyunina ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Bone Tissue ◽  
Tissue Engineering Construct ◽  
Tissue Cells ◽  
Adipose Tissue Cells

scholarly journals Moscow State University of Medicine and Dentistry named after A.I. Evdokimov

2021 ◽  
Vol 19 (4) ◽  
pp. 343-349
Author(s):  
G. A. Volozhin ◽  
E. A. Bazikian ◽  
R. V. Deev ◽  
I. E. Bozo ◽  
E. A. Presnyakov
Keyword(s):  
Tissue Engineering ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Bone Tissue ◽  
Plasmid Dna ◽  
Octacalcium Phosphate ◽  
Vascular Endothelial ◽  
Growth Factor Gene ◽  
Tissue Engineering Construct ◽  
Endothelial Growth Factor

Relevance. The effect of a tissue engineering construct based on synthetic octacalcium phosphate activated with plasmid DNA with vascular endothelial growth factor gene on bone morphogenesis at the jaw defect sites of patients was studied. It is shown that the studied osteoplastic material stimulates osteosynthesis pathways already at early stages, and xenogenic hydroxyapatite, triggers osteogenesis processes with considerable delay and does not have time to form a full-fledged bone structure by 6 months.Aim. Evaluate the dynamics of reparative osteogenesis based on the results of histomorphometric diagnostics in patients with defects in the jaw bones of various configurations and lengths with an implanted bone matrix based on synthetic octacalcium phosphate activated with plasmid deoxyribonucleic acid with genes of vascular endothelial growth factor.Materials and methods. Histomorphological examination of bone tissue biopsy of jaws was carried out in 50 patients of both genders, who needed additional volume of bone tissue to install dental implantation. Patients were divided into 2 groups by type of grafted material. 6 months after the surgery, bone biopsies were taken from the bone sites at the stage of implant placement. Histomorphological patterns and histomycrophotograms were studied in bone biopcies.Results. In bone tissue biopsies of patients implanted with a tissue engineering construct based on synthetic octacalcium phosphate activated with plasmid DNA with vascular endothelial growth factor gene, it was revealed that after 6 months there was prevaluation of mature bone tissue (42,71%), with the proportion of differentiated plate bone tissue being < 90%.Conclusions. Histomorphometric analysis showed that in patients implanted with a tissue engineering construct based on synthetic octacalcium phosphate activated with plasmid DNA with vascular endothelial growth factor gene, after 6 months, early rearrangement of bone tissue into a mechanically dense and highly mineralized structure was detected. 

scholarly journals A bioactive hybrid three-dimensional tissue-engineering construct for cartilage repair

2015 ◽  
Vol 30 (6) ◽  
pp. 873-885 ◽  
Author(s):  
Mari Ainola ◽  
Waclaw Tomaszewski ◽  
Barbara Ostrowska ◽  
Ewa Wesolowska ◽  
H Daniel Wagner ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Repair ◽  
Three Dimensional ◽  
Tissue Engineering Construct

scholarly journals 3D-printed β-TCP bone tissue engineering scaffolds: Effects of chemistry on in vivo biological properties in a rabbit tibia model

2018 ◽  
Vol 33 (14) ◽  
pp. 1939-1947 ◽  
Author(s):  
Samit Kumar Nandi ◽  
Gary Fielding ◽  
Dishary Banerjee ◽  
Amit Bandyopadhyay ◽  
Susmita Bose
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biological Properties ◽  
Tissue Engineering Scaffolds ◽  
Rabbit Tibia ◽  
3D Printed ◽  
Image Position

Abstract

scholarly journals ISLET CELL CULTURES AS COMPONENT OF TISSUE-ENGINEERING CONSTRUCT OF PANCREAS

2018 ◽  
Vol 20 (2) ◽  
pp. 74-81
Author(s):  
N. N. Skaletskiy ◽  
G. N. Skaletskaya ◽  
L. A. Kirsanova ◽  
N. V. Baranova ◽  
G. N. Bubentsova ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Cell Cultures ◽  
Islet Cell ◽  
Morphological Analysis ◽  
Pancreatic Tissue ◽  
Cell Component ◽  
Inverted Microscope ◽  
Tissue Engineering Construct ◽  
Immunohistochemical Methods

Aim:to develop methods for obtaining islet cell cultures for the purpose of their further use as a suitable component of the pancreatic tissue engineered construct. As a source of islet cell cultures, pancreas of newborn rabbits was used as an accessible and well-studied donor model.Materials and methods.For the obtaining of islet cell cultures, pancreas of 1–3-day-old newborn rabbits were used. Changes occurring during the cultivation of pancreatic tissue were recorded using an inverted microscope and a biostation. Morphological analysis of culture samples was carried out using histological and specifi c immunohistochemical methods. The insulin-producing activity of the cultures was determined by enzyme immunoassay.Results.Three main types of cultures were obtained: isletlike organotypic, suspensionalcytotypic, and monolayered, consisting of progenitor cells. Greater morphological safety and adequate insulin-producing ability was revealed in fl oating islet-like cultures.Conclusion.According to their morphofunctional properties, fl otation islet-like cultures obtained from pancreas of the newborn rabbits can be used as basal cell component of the experimental model of the tissue engineered construct of the pancreas. 

A Hybrid Nano-Bioprinting Device for Tissue Engineering

2010 ◽  
Author(s):  
Kivilcim Buyukhatipoglu ◽  
Wei Sun ◽  
Alisa Morss Clyne
Keyword(s):  
Tissue Engineering ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Tissue Growth ◽  
Superparamagnetic Nanoparticles ◽  
Oxide Nanoparticles ◽  
3D Architecture ◽  
Tissue Engineering Construct ◽  
Direct Cell

Tissue engineering may require precise patterning of cells and bioactive factors to recreate the complex, 3D architecture of native tissue. These cells and bioactive components may then need to be repositioned during tissue growth in vitro and noninvasively imaged to track tissue development. We developed a new hybrid nano-bioprinting system by combining the initial patterning capabilities of a direct cell writing system with the active patterning capabilities of superparamagnetic nanoparticles. The iron oxide nanoparticles can be conjugated with proteins or loaded inside cells, printed into computer-defined patterns, and then manipulated and imaged within the 3D tissue engineering construct. In this study, iron oxide nanoparticles were bioprinted either in an alginate scaffold or inside endothelial cells. Cell viability, patterning, and imaging were assessed.

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