Human Amniotic Cell Sheet Harvest Using a Novel Temperature-Responsive Culture Surface Coated with Protein-Based Polymer

2006 ◽  
Vol 12 (2) ◽  
pp. 391-401 ◽  
Author(s):  
Helin Zhang ◽  
Masamichi Iwama ◽  
Toshihiro Akaike ◽  
Dan W. Urry ◽  
Asima Pattanaik ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Temperature Responsive ◽  
Amniotic Cell ◽  
Culture Surface

Human Amniotic Cell Sheet Harvest Using a Novel Temperature-Responsive Culture Surface Coated with Protein-Based Polymer

2006 ◽  
Vol 0 (0) ◽  
pp. 060224120000002
Author(s):  
Helin Zhang ◽  
Masamichi Iwama ◽  
Toshihiro Akaike ◽  
Dan W. Urry ◽  
Asima Pattanaik ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Temperature Responsive ◽  
Amniotic Cell ◽  
Culture Surface

scholarly journals Design of Temperature-Responsive Cell Culture Surfaces for Cell Sheet Engineering

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Y. Akiyama
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Cell Sheet ◽  
Extensive Study ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Interdisciplinary Field ◽  
Culture Surface ◽  
Responsive Cell

Temperature-responsive cell culture surfaces, which modulate cell attachment/detachment characteristics with temperature, have been used to fabricate cell sheets. Extensive study on fabrication of cell sheet with the temperature-responsive cell culture surface, manipulation, and transplantation of the cell sheet has established the interdisciplinary field of cell sheet engineering, in which engineering, biological, and medical fields closely collaborate. Such collaboration has pioneered cell sheet engineering, making it a promising and attractive technology in tissue engineering and regenerative medicine. This review introduces concepts of cell sheet engineering, followed by designs for the fabrication of various types of temperature-responsive cell culture surfaces and technologies for cell sheet manipulation. The development of various methods for the fabrication of temperature-responsive cell culture surfaces was also summarized. The availability of cell sheet engineering for the treatment and regeneration of damaged human tissue has also been described, providing examples of the clinical application of cell sheet transplantation in humans.

scholarly journals Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering

2009 ◽  
Vol 6 (suppl_3) ◽  
Author(s):  
Kenichi Nagase ◽  
Jun Kobayashi ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Copolymer Composition ◽  
Cell Sheets ◽  
Chromatographic Separations ◽  
Cell Sheet Engineering ◽  
External Temperature ◽  
Cell Monolayers ◽  
Temperature Responsive ◽  
Confluent Cell ◽  
Dependent Cell

Temperature-responsive intelligent surfaces, prepared by the modification of an interface with poly( N -isopropylacrylamide) and its derivatives, have been used for biomedical applications. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated interactions with biomolecules and cells. In this review, we focus on the application of these intelligent surfaces to chromatographic separation and cell cultures. Chromatographic separations using several types of intelligent surfaces are mentioned briefly, and various effects related to the separation of bioactive compounds are discussed, including wettability, copolymer composition and graft polymer architecture. Similarly, we also summarize temperature-responsive cell culture substrates that allow the recovery of confluent cell monolayers as contiguous living cell sheets for tissue-engineering applications. The key factors in temperature-dependent cell adhesion/detachment control are discussed from the viewpoint of grafting temperature-responsive polymers, and new methodologies for effective cell sheet culturing and the construction of thick tissues are summarized.

scholarly journals Surface modification of uniaxial cyclic strain cell culture platform with temperature-responsive polymer for cell sheet detachment

2015 ◽  
Vol 3 (40) ◽  
pp. 7899-7902 ◽  
Author(s):  
E. L. Lee ◽  
H. H. Bendre ◽  
A. Kalmykov ◽  
J. Y. Wong
Keyword(s):  
Cell Culture ◽  
Intact Cell ◽  
Cell Sheet ◽  
Cyclic Strain ◽  
Cell Phenotype ◽  
Temperature Responsive ◽  
Current Cell ◽  
Responsive Polymer ◽  
Smooth Muscle Cell Phenotype ◽  
A Cell

Because current cell sheet-based blood vessels lack biomimetic structure and require excessively long culture times that may compromise smooth muscle cell phenotype, we modified a cell culture platform with thermoresponsive copolymers to allow intact cell sheet detachment after uniaxial conditioning.

scholarly journals Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering

Polymers ◽  
2012 ◽  
Vol 4 (3) ◽  
pp. 1478-1498 ◽  
Author(s):  
Zhonglan Tang ◽  
Yoshikatsu Akiyama ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Responsive Polymer ◽  
Modified Surface
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