Rapid Differentiation of Multi-Zone Ocular Cells from Human Induced Pluripotent Stem Cells and Generation of Corneal Epithelial and Endothelial Cells

2019 ◽  
Vol 28 (7) ◽  
pp. 454-463 ◽  
Author(s):  
Zongyi Li ◽  
Haoyun Duan ◽  
Wenjing Li ◽  
Xiaoli Hu ◽  
Yanni Jia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Corneal Epithelial ◽  
Induced Pluripotent

scholarly journals Automated Deep Learning-Based System to Identify Endothelial Cells Derived from Induced Pluripotent Stem Cells

2018 ◽  
Vol 10 (6) ◽  
pp. 1687-1695 ◽  
Author(s):  
Dai Kusumoto ◽  
Mark Lachmann ◽  
Takeshi Kunihiro ◽  
Shinsuke Yuasa ◽  
Yoshikazu Kishino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Deep Learning ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Induced Pluripotent Stem Cells as Vasculature Forming Entities

2019 ◽  
Vol 8 (11) ◽  
pp. 1782 ◽  
Author(s):  
Antonio Palladino ◽  
Isabella Mavaro ◽  
Carmela Pizzoleo ◽  
Elena De Felice ◽  
Carla Lucini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Regenerative Medicine ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Tissue Growth ◽  
Safety Issues ◽  
Induced Pluripotent

Tissue engineering (TE) pursues the ambitious goal to heal damaged tissues. One of the most successful TE approaches relies on the use of scaffolds specifically designed and fabricated to promote tissue growth. During regeneration the guidance of biological events may be essential to sustain vasculature neoformation inside the engineered scaffold. In this context, one of the most effective strategies includes the incorporation of vasculature forming cells, namely endothelial cells (EC), into engineered constructs. However, the most common EC sources currently available, intended as primary cells, are affected by several limitations that make them inappropriate to personalized medicine. Human induced Pluripotent Stem Cells (hiPSC), since the time of their discovery, represent an unprecedented opportunity for regenerative medicine applications. Unfortunately, human induced Pluripotent Stem Cells-Endothelial Cells (hiPSC-ECs) still display significant safety issues. In this work, we reviewed the most effective protocols to induce pluripotency, to generate cells displaying the endothelial phenotype and to perform an efficient and safe cell selection. We also provide noteworthy examples of both in vitro and in vivo applications of hiPSC-ECs in order to highlight their ability to form functional blood vessels. In conclusion, we propose hiPSC-ECs as the preferred source of endothelial cells currently available in the field of personalized regenerative medicine.

scholarly journals Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells

2018 ◽  
Vol 10 (5) ◽  
pp. 1642-1656 ◽  
Author(s):  
Oleh V. Halaidych ◽  
Christian Freund ◽  
Francijna van den Hil ◽  
Daniela C.F. Salvatori ◽  
Mara Riminucci ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Barrier Function ◽  
Inflammatory Responses ◽  
Induced Pluripotent
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