scholarly journals Differentiation of Urine-Derived Induced Pluripotent Stem Cells to Neurons, Astrocytes, and Microvascular Endothelial Cells from a Diabetic Patient

2020 ◽  
Vol 22 (3) ◽  
pp. 147-155
Author(s):  
Wan Liu ◽  
Ping Zhang ◽  
Jing Tan ◽  
Yongzhong Lin
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Diabetic Patient ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial ◽  
Induced Pluripotent

scholarly journals Differentiation of Urine-Derived Induced Pluripotent Stem Cells to Neuron, Astrocyte and Microvascular Endothelial Cells from a Diabetic Patient

2019 ◽  
Author(s):  
Wan Liu ◽  
Ping Zhang ◽  
Jing Tan ◽  
Yongzhong Lin
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Disease Model ◽  
Morphological Characteristics ◽  
Microvascular Endothelial Cells ◽  
Alkaline Phosphatase Staining ◽  
Microvascular Endothelial ◽  
Induced Pluripotent

AbstractBackgroundComplications of central nervous system (CNS) in type 2 diabetes mellitus (T2DM) often lead to cognitive impairment and seriously affect the quality of life. However, there is no individualized disease model. Urinary epithelial cells (UECs) can be an ideal source for generating human induced pluripotent stem cells (hiPSCs) and progenitors, as they are easily accessible, non-invasive and universally available. Therefore, we intended to differentiate urine-derived hiPSCs into neuron (N), astrocyte (A) and microvascular endothelial cells (E) from a T2DM patient for future study its pathogenesis and precision medical treatment.Methods and ResultshiPSCs was successfully induced from UECs using integration free Sendai virus technology in a totally noninvasive manner. It had a normal karyotype (46, XY) and were proved to be pluripotent by immunofluorescence staining, alkaline phosphatase staining, karyotyping, teratoma experiments and methylated analysis. N, A and E were successfully induced and displayed typical morphological characteristics.ConclusionsThis study indicates that N, A, E can be generated from urine-derived hiPSCs. Then we intend to create a new disease model in vitro to simulate the cerebral microenvironment of DM which will provide new methods for further investigate the disease-specific mechanisms.

scholarly journals Automated Deep Learning-Based System to Identify Endothelial Cells Derived from Induced Pluripotent Stem Cells

2018 ◽  
Vol 10 (6) ◽  
pp. 1687-1695 ◽  
Author(s):  
Dai Kusumoto ◽  
Mark Lachmann ◽  
Takeshi Kunihiro ◽  
Shinsuke Yuasa ◽  
Yoshikazu Kishino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Deep Learning ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Induced Pluripotent Stem Cells as Vasculature Forming Entities

2019 ◽  
Vol 8 (11) ◽  
pp. 1782 ◽  
Author(s):  
Antonio Palladino ◽  
Isabella Mavaro ◽  
Carmela Pizzoleo ◽  
Elena De Felice ◽  
Carla Lucini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Regenerative Medicine ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Tissue Growth ◽  
Safety Issues ◽  
Induced Pluripotent

Tissue engineering (TE) pursues the ambitious goal to heal damaged tissues. One of the most successful TE approaches relies on the use of scaffolds specifically designed and fabricated to promote tissue growth. During regeneration the guidance of biological events may be essential to sustain vasculature neoformation inside the engineered scaffold. In this context, one of the most effective strategies includes the incorporation of vasculature forming cells, namely endothelial cells (EC), into engineered constructs. However, the most common EC sources currently available, intended as primary cells, are affected by several limitations that make them inappropriate to personalized medicine. Human induced Pluripotent Stem Cells (hiPSC), since the time of their discovery, represent an unprecedented opportunity for regenerative medicine applications. Unfortunately, human induced Pluripotent Stem Cells-Endothelial Cells (hiPSC-ECs) still display significant safety issues. In this work, we reviewed the most effective protocols to induce pluripotency, to generate cells displaying the endothelial phenotype and to perform an efficient and safe cell selection. We also provide noteworthy examples of both in vitro and in vivo applications of hiPSC-ECs in order to highlight their ability to form functional blood vessels. In conclusion, we propose hiPSC-ECs as the preferred source of endothelial cells currently available in the field of personalized regenerative medicine.

scholarly journals Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells

2018 ◽  
Vol 10 (5) ◽  
pp. 1642-1656 ◽  
Author(s):  
Oleh V. Halaidych ◽  
Christian Freund ◽  
Francijna van den Hil ◽  
Daniela C.F. Salvatori ◽  
Mara Riminucci ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Barrier Function ◽  
Inflammatory Responses ◽  
Induced Pluripotent
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