Isolation and Characterization of Mesenchymal Stem Cells from the Fat Layer on the Density Gradient Separated Bone Marrow

2012 ◽  
Vol 21 (2) ◽  
pp. 260-272 ◽  
Author(s):  
Carmen L. Insausti ◽  
Miguel Blanquer Blanquer ◽  
Luis Meseguer Olmo ◽  
María C. López-Martínez ◽  
Xavier Férez Ruiz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Density Gradient ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal stem cells in Xenopus laevis

2021 ◽  
pp. 102341
Author(s):  
Rina Otsuka-Yamaguchi ◽  
Masaaki Kitada ◽  
Yasumasa Kuroda ◽  
Yoshihiro Kushida ◽  
Shohei Wakao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Xenopus Laevis ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of turkey bone marrow‐derived mesenchymal stem cells

2019 ◽  
Vol 37 (6) ◽  
pp. 1419-1428
Author(s):  
Qian Liu ◽  
Yaxi Zhu ◽  
Jun Qi ◽  
Peter C. Amadio ◽  
Steven L. Moran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Isolation And Characterization

scholarly journals A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis

2016 ◽  
Vol 2016 ◽  
pp. 1-18 ◽  
Author(s):  
Subhash C. Juneja ◽  
Sowmya Viswanathan ◽  
Milan Ganguly ◽  
Christian Veillette
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
Isolation And Characterization ◽  
Standard Operating ◽  
Total Knee ◽  
Detailed Protocol

The procedure for aspiration of bone marrow from the femur of patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA) may vary from an OR (operating room) to OR based on the surgeon’s skill and may lead to varied extent of clotting of the marrow and this, in turn, presents difficulty in the isolation of mesenchymal stem cells (MSCs) from such clotted bone marrow. We present a simple detailed protocol for aspirating bone marrow from such patients, isolation, and characterization of MSCs from the aspirated bone marrow specimens and show that the bone marrow presented no clotting or exhibited minimal clotting. This represents an economical source and convenient source of MSCs from bone marrow for use in regenerative medicine. Also, we presented the detailed protocol and showed that the MSCs derived from such bone marrow specimens exhibited MSCs characteristics and generated micromass cartilages, the recipe for regenerative medicine for osteoarthritis. The protocols we presented can be used as standard operating procedures (SOPs) by researchers and clinicians.

Isolation and characterization of multipotential mesenchymal stem cells from feline bone marrow

2002 ◽  
Vol 30 (8) ◽  
pp. 879-886 ◽  
Author(s):  
Douglas R Martin ◽  
Nancy R Cox ◽  
Terri L Hathcock ◽  
Glenn P Niemeyer ◽  
Henry J Baker
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Isolation And Characterization

Isolation and characterization of mesenchymal stem cells from chicken bone marrow

2012 ◽  
Vol 14 (3) ◽  
pp. 437-451 ◽  
Author(s):  
Chunyu Bai ◽  
Lingling Hou ◽  
Yuehui Ma ◽  
Lina Chen ◽  
Minghai Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Isolation And Characterization ◽  
Chicken Bone

Isolation and characterization of hypoxic cultured bone marrow derived mesenchymal stem cells from yorkshire pigs

Cytotherapy ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. S47-S48
Author(s):  
Francisco Silva ◽  
Mark Urtz ◽  
Thomas Hoffman ◽  
Christian Elbad ◽  
Gregory Lutz
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Isolation And Characterization ◽  
Yorkshire Pigs

Isolation and characterization of bone marrow–derived equine mesenchymal stem cells

2007 ◽  
Vol 68 (10) ◽  
pp. 1095-1105 ◽  
Author(s):  
Stefan J. Arnhold ◽  
Iris Goletz ◽  
Helmut Klein ◽  
Gerald Stumpf ◽  
Lisa A. Beluche ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Isolation And Characterization

Isolation and Characterization of Multipotent Mesenchymal Stem Cells Adhering to Adipocytes in Canine Bone Marrow

2017 ◽  
Vol 26 (6) ◽  
pp. 431-440 ◽  
Author(s):  
Hsing-Yi Lin ◽  
Naoki Fujita ◽  
Kentaro Endo ◽  
Maresuke Morita ◽  
Tae Takeda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Isolation And Characterization ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals Canine colostrum exosomes: characterization, anti-oxidative capacity and influence on canine mesenchymal stem cell

2019 ◽  
Author(s):  
Antonio J Villatoro ◽  
María del Carmen Martín-Astorga ◽  
Cristina Alcoholado ◽  
José Becerra
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Oxidative Capacity ◽  
Antioxidant Effect ◽  
Bioactive Molecules ◽  
Passive Immunity ◽  
Isolation And Characterization ◽  
First Time

Abstract Background: Colostrum is a specific secretion of the mammary gland fundamental for the survival of the puppy during the first weeks after birth. It contains important bioactive molecules involved in the passive immunity and the maturation of various organs, highlighting small vesicles named exosomes. Exosomes have not been described yet in canine colostrum milk, where its role is crucial in dam-newborn communication for the development of the neonate. Results: Exosomes were abundant in canine colostrum milk and appeared with a characteristic cup-shaped morphology and well-defined round vesicles. Their size distribution was between 37−140 nm and western blot analysis showed an expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosomes cargo. We also found that exosomes modified proliferation and secretory profile in canine mesenchymal stem cells derived from bone marrow and adipose tissue, differently according to their origin. Besides, the exosomes of canine colostrum demonstrated a potent antioxidant effect. Conclusions: We described for the first time the isolation and characterization of milk exosomes from canine colostrum. Our findings highlight the abundant presence of exosomes in the colostrum of the canine species and contribute to explain their important antioxidant capacity after parturition and their role in the modulation of cell development and tissue differentiation in the newborn, where mesenchymal stem cells seem to play a key role.

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