Isolation and Characterization of Multipotent Mesenchymal Stem Cells Adhering to Adipocytes in Canine Bone Marrow

2017 ◽  
Vol 26 (6) ◽  
pp. 431-440 ◽  
Author(s):  
Hsing-Yi Lin ◽  
Naoki Fujita ◽  
Kentaro Endo ◽  
Maresuke Morita ◽  
Tae Takeda ◽  
...  
2021 ◽  
pp. 102341
Author(s):  
Rina Otsuka-Yamaguchi ◽  
Masaaki Kitada ◽  
Yasumasa Kuroda ◽  
Yoshihiro Kushida ◽  
Shohei Wakao ◽  
...  

2019 ◽  
Vol 37 (6) ◽  
pp. 1419-1428
Author(s):  
Qian Liu ◽  
Yaxi Zhu ◽  
Jun Qi ◽  
Peter C. Amadio ◽  
Steven L. Moran ◽  
...  

2014 ◽  
Vol 240 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Jung-Sun Cho ◽  
Joo-Hoo Park ◽  
Ju-Hyung Kang ◽  
Sung Eun Kim ◽  
Il-Ho Park ◽  
...  

2016 ◽  
Vol 2016 ◽  
pp. 1-18 ◽  
Author(s):  
Subhash C. Juneja ◽  
Sowmya Viswanathan ◽  
Milan Ganguly ◽  
Christian Veillette

The procedure for aspiration of bone marrow from the femur of patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA) may vary from an OR (operating room) to OR based on the surgeon’s skill and may lead to varied extent of clotting of the marrow and this, in turn, presents difficulty in the isolation of mesenchymal stem cells (MSCs) from such clotted bone marrow. We present a simple detailed protocol for aspirating bone marrow from such patients, isolation, and characterization of MSCs from the aspirated bone marrow specimens and show that the bone marrow presented no clotting or exhibited minimal clotting. This represents an economical source and convenient source of MSCs from bone marrow for use in regenerative medicine. Also, we presented the detailed protocol and showed that the MSCs derived from such bone marrow specimens exhibited MSCs characteristics and generated micromass cartilages, the recipe for regenerative medicine for osteoarthritis. The protocols we presented can be used as standard operating procedures (SOPs) by researchers and clinicians.


2002 ◽  
Vol 30 (8) ◽  
pp. 879-886 ◽  
Author(s):  
Douglas R Martin ◽  
Nancy R Cox ◽  
Terri L Hathcock ◽  
Glenn P Niemeyer ◽  
Henry J Baker

2012 ◽  
Vol 14 (3) ◽  
pp. 437-451 ◽  
Author(s):  
Chunyu Bai ◽  
Lingling Hou ◽  
Yuehui Ma ◽  
Lina Chen ◽  
Minghai Zhang ◽  
...  

Cytotherapy ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. S47-S48
Author(s):  
Francisco Silva ◽  
Mark Urtz ◽  
Thomas Hoffman ◽  
Christian Elbad ◽  
Gregory Lutz

2007 ◽  
Vol 68 (10) ◽  
pp. 1095-1105 ◽  
Author(s):  
Stefan J. Arnhold ◽  
Iris Goletz ◽  
Helmut Klein ◽  
Gerald Stumpf ◽  
Lisa A. Beluche ◽  
...  

2005 ◽  
Vol 14 (4) ◽  
pp. 440-451 ◽  
Author(s):  
Reza Izadpanah ◽  
Tanya Joswig ◽  
Fern Tsien ◽  
Jason Dufour ◽  
J. Chrisitian Kirijan ◽  
...  

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