scholarly journals Human Liver Progenitor Cell Lines Are Readily Established From Non-Tumorous Tissue Adjacent to Hepatocellular Carcinoma

2010 ◽  
Vol 19 (8) ◽  
pp. 1277-1284 ◽  
Author(s):  
Aibin Zhang ◽  
Roslyn London ◽  
Frederik Michael Schulz ◽  
Philippe Wilfred Roger Giguère-Simmonds ◽  
Luc Delriviere ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Lines ◽  
Human Liver ◽  
Progenitor Cell ◽  
Liver Progenitor Cell

scholarly journals Epigenetic Modulators Enhance Constitutive and Liver-Specific Reporter Expression in Murine Liver Progenitor Cell Lines

2015 ◽  
Vol 21 (10) ◽  
pp. 1080-1087 ◽  
Author(s):  
Luke Aris Diepeveen ◽  
Michel Elyse Watson ◽  
Sarah Beth McSpadden ◽  
Robyn Patricia Strauss ◽  
Bernard Andrew Callus ◽  
...  
Keyword(s):  
Cell Lines ◽  
Progenitor Cell ◽  
Reporter Expression ◽  
Murine Liver ◽  
Liver Progenitor Cell

scholarly journals β-Catenin Activation in a Novel Liver Progenitor Cell Type Is Sufficient to Cause Hepatocellular Carcinoma and Hepatoblastoma

2014 ◽  
Vol 74 (16) ◽  
pp. 4515-4525 ◽  
Author(s):  
Sharada Mokkapati ◽  
Katharina Niopek ◽  
Le Huang ◽  
Kegan J. Cunniff ◽  
E. Cristy Ruteshouser ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Progenitor Cell ◽  
Cell Type ◽  
Liver Progenitor Cell

scholarly journals Antiproliferative Activity Evaluation of a Series ofN-1,3-Benzothiazol-2-ylbenzamides as Novel Apoptosis Inducers

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Filomena Corbo ◽  
Alessia Carocci ◽  
Domenico Armenise ◽  
Nicolino De Laurentis ◽  
Antonio Laghezza ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Hepatocellular Carcinoma ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Human Breast Cancer ◽  
Human Liver ◽  
Inhibitory Effect ◽  
Human Breast ◽  
Liver Hepatocellular Carcinoma ◽  
Mcf 7

A series ofN-1,3-benzothiazol-2-ylbenzamide derivatives were studied for their antiproliferative activity on human liver hepatocellular carcinoma (HepG2) and human breast cancer (MCF-7) cell lines. Most of them were found to show a prominent inhibitory effect on cell growth. Among the most active compounds,1kemerged for its proapoptotic effect that is particularly evident towards MCF-7 cancer cell lines.

scholarly journals ImmunoPET as Stoichiometric Sensor for Glypican-3 in Models of Hepatocellular Carcinoma

2020 ◽  
Author(s):  
Olivia J. Kelada ◽  
Nicholas T. Gutsche ◽  
Meghan Bell ◽  
Rose M. Berman ◽  
Kwamena E. Baidoo ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Flow Cytometry ◽  
Liver Cancer ◽  
Cell Lines ◽  
Human Liver ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Human Liver Cancer ◽  
Gpc3 Expression

BackgroundHepatocellular carcinoma (HCC) is the fifth most common cancer worldwide. While conventional imaging approaches like ultrasound, CT, and MRI play critical roles in the diagnosis and surveillance of HCC, improved methods for detection and assessment of treatment response are needed. One promising approach is the use of radiolabeled antibodies for positron emission tomography (immunoPET) imaging. Glypican-3 (GPC3) is a proteoglycan that is highly expressed in the majority of HCC tumors. GPC3-specific antibodies are used to diagnose HCC histopathologically, and have been proposed as a treatment of HCC. Here, we design, synthesize and demonstrate that our humanized immunoPET agent, [89Zr]Zr-DFO-TAB-H14, can stoichiometrically bind to models of human liver cancer with varied GPC3 expression. Methods: The GPC3-specific monoclonal humanized IgG1, TAB-H14, was used as a scaffold for engineering our immunoPET agent. Fluorescent and deferroxamine (DFO) chelate conjugates of TAB-H14 were characterized using mass spectrometry. Binding affinity of TAB-H14 and conjugates for GPC3 was determined in cell-free biolayer interferometry, and cell-based radioimmunoassays. GPC3-expression was assessed by flow cytometry and immunofluorescence using commercially available anti-GPC3 antibodies and TAB-H14 in GPC3−(A431) and GPC3+ cell lines including an engineered line (A431-GPC3+, G1) and liver cancer lines (HepG2, Hep3B, and Huh7). DFO-TAB-H14, was radiolabeled with Zr-89. Mice were subcutaneously engrafted with the aforementioned cell lines and in vivo target engagement of the immunoPET agent [89Zr]Zr-DFO-TAB-H14 was determined using PET/CT, quantitative biodistribution, and autoradiography. Results: TAB-H14 demonstrated subnanomolar to nanomolar affinity for human GPC3. Fluorescently tagged TAB-H14 was able to bind to GPC3 on cell membranes of GPC3-expressing lines by flow cytometry. These results were confirmed by immunofluorescence staining of A431, G1 HepG2, Hep3B, and Huh7 tumor sections. ImmunoPET imaging with [89Zr]Zr-DFO-TAB-H14 showed stoichiometric tumor uptake corresponding to the cell surface expression levels. Autoradiography and immunostaining confirmed in vivo findings. Conclusion: We systematically demonstrate that the humanized immnoPET agent [89Zr]Zr-DFO-TAB-H14 specifically and stoichiometrically binds to GPC3 in several models of human liver cancer, serving as a promising in vivo GPC3 sensor. This agent may provide utility in HCC diagnosis and surveillance, and the selection of candidates for GPC3-directed therapies.

scholarly journals Characterisation of a stereotypical cellular and extracellular adult liver progenitor cell niche in rodents and diseased human liver

Gut ◽  
2010 ◽  
Vol 59 (5) ◽  
pp. 645-654 ◽  
Author(s):  
S. Lorenzini ◽  
T. G. Bird ◽  
L. Boulter ◽  
C. Bellamy ◽  
K. Samuel ◽  
...  
Keyword(s):  
Human Liver ◽  
Progenitor Cell ◽  
Adult Liver ◽  
Cell Niche ◽  
Liver Progenitor Cell

scholarly journals Human Liver Progenitor Cell Expansion With Preservation of Differentiation Capability Into Functional Hepatocytes

2011 ◽  
Vol 140 (5) ◽  
pp. S-976
Author(s):  
Dustin T. Boyd ◽  
Melissa R. Miller ◽  
Woodring E. Wright ◽  
Jay D. Horton ◽  
Jerry W. Shay ◽  
...  
Keyword(s):  
Cell Expansion ◽  
Human Liver ◽  
Progenitor Cell ◽  
Liver Progenitor Cell

Differentiation of the human liver progenitor cell line (HepaRG) on a microfluidic‐based biochip

2019 ◽  
Vol 13 (3) ◽  
pp. 482-494 ◽  
Author(s):  
Mi Jang ◽  
Astrid Kleber ◽  
Thomas Ruckelshausen ◽  
Ralf Betzholz ◽  
Andreas Manz
Keyword(s):  
Cell Line ◽  
Human Liver ◽  
Progenitor Cell ◽  
Liver Progenitor Cell ◽  
Progenitor Cell Line
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