Effect of Storage Time, Postmortem Interval, Agonal State, and Gender on the Postmortem Preservation of Glial Fibrillary Acidic Protein and Oxidatively Damaged Proteins in Human Brains

2011 ◽  
Vol 9 (4) ◽  
pp. 379-387 ◽  
Author(s):  
G. Harish ◽  
C. Venkateshappa ◽  
Anita Mahadevan ◽  
Nupur Pruthi ◽  
M.M. Srinivas Bharath ◽  
...  
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Storage Time ◽  
Postmortem Interval ◽  
Acidic Protein ◽  
And Gender ◽  
Human Brains ◽  
Agonal State

scholarly journals Reduced astrocytic reactivity in human brains and midbrain organoids with PRKN mutations

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Masayoshi Kano ◽  
Masashi Takanashi ◽  
Genko Oyama ◽  
Asako Yoritaka ◽  
Taku Hatano ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Glial Fibrillary Acidic Protein ◽  
Dopaminergic Neurons ◽  
Cell Loss ◽  
Acidic Protein ◽  
Patient Specific ◽  
Dopaminergic Cell ◽  
Induced Pluripotent ◽  
Human Brains ◽  
Defective Clearance

AbstractParkin (encoded by PRKN) is a ubiquitin ligase that plays an important role in cellular mitochondrial quality control. Mutations in PRKN cause selective dopaminergic cell loss in the substantia nigra and are presumed to induce a decrease in mitochondrial function caused by the defective clearance of mitochondria. Several studies have demonstrated that parkin dysfunction causes mitochondrial injury and astrocytic dysfunction. Using immunohistochemical methods, we analyzed astrocytic changes in human brains from individuals with PRKN mutations. Few glial fibrillary acidic protein- and vimentin-positive astrocytes were observed in the substantia nigra in PRKN-mutated subjects compared with subjects with idiopathic Parkinson’s disease. We also differentiated patient-specific induced pluripotent stem cells into midbrain organoids and confirmed decreased numbers of glial fibrillary acidic protein-positive astrocytes in PRKN-mutated organoids compared with age- and sex-matched controls. Our study reveals PRKN-mutation-induced astrocytic alteration and suggests the possibility of an astrocyte-related non-autonomous cell death mechanism for dopaminergic neurons in brains of PRKN-mutated patients.

Glutathione metabolism is modulated by postmortem interval, gender difference and agonal state in postmortem human brains

2011 ◽  
Vol 59 (7) ◽  
pp. 1029-1042 ◽  
Author(s):  
G. Harish ◽  
C. Venkateshappa ◽  
Anita Mahadevan ◽  
Nupur Pruthi ◽  
M.M. Srinivas Bharath ◽  
...  
Keyword(s):  
Gender Difference ◽  
Postmortem Interval ◽  
Glutathione Metabolism ◽  
Human Brains ◽  
Agonal State

Expression of glial fibrillary acidic protein, actin, fibronectin and factor VIII antigen in human astrocytomas

Pathology ◽  
1983 ◽  
Vol 15 (4) ◽  
pp. 373-378 ◽  
Author(s):  
Stephen J. Lolait ◽  
J.H. Harmer ◽  
G. Auteri ◽  
J.S. Pedersen ◽  
B.H. Toh
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Factor Viii ◽  
Acidic Protein ◽  
Factor Viii Antigen ◽  
Human Astrocytomas

scholarly journals Changes in Plasma Glial Fibrillary Acidic Protein in Children Receiving Sevoflurane Anesthesia: A Preliminary Randomized Trial

2021 ◽  
Vol 10 (4) ◽  
pp. 662
Author(s):  
Eun-Hee Kim ◽  
Young-Eun Jang ◽  
Sang-Hwan Ji ◽  
Ji-Hyun Lee ◽  
Sung-Ae Cho ◽  
...  
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Randomized Trial ◽  
Protein Concentration ◽  
Pediatric Patients ◽  
Neuronal Injury ◽  
Acidic Protein ◽  
Anesthesia Induction ◽  
Sevoflurane Anesthesia ◽  
Control Groups ◽  
Over Time

We investigated changes in plasma glial fibrillary acidic protein concentration during sevoflurane anesthesia induction in children < 3 years old and determined the effect of co-administering dexmedetomidine. This preliminary randomized trial included 60 pediatric patients who received sevoflurane anesthesia for >3 h. Patients were assigned to dexmedetomidine or control groups at a 1:1 ratio. The primary outcome was changes in plasma glial fibrillary acidic protein concentration of dexmedetomidine and control groups over time. Fifty-five patients were included in the final analysis. The median (interquartile range (IQR)) of the plasma glial fibrillary acidic protein level was 387.7 (298.9–510.8) pg·mL−1 immediately after anesthetic induction, 302.6 (250.9–412.5) pg·mL−1 at 30 min, and 321.9 (233.8–576.2) pg·mL−1 at 180 min after the first sample. These values did not change over time (p = 0.759). However, plasma glial fibrillary acidic protein increased after 180 min of infusion of dexmedetomidine compared with values at 30 min infusion (p = 0.04, mean difference and 95% confidence interval of 221.6 and 2.2 to 441.0 pg·mL−1). In conclusion, three hours of sevoflurane anesthesia in pediatric patients < 3 years old did not provoke neuronal injury assessed by the plasma biomarker. Further studies regarding the effect of prolonged dexmedetomidine infusion on anesthetic neuronal injury are required.

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