scholarly journals Hot Spring Microbial Community Elemental Composition: Hot Spring and Soil Inputs, and the Transition from Biocumulus to Siliceous Sinter

Astrobiology ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 1526-1546
Author(s):  
Jeff R. Havig ◽  
Joshua E. Kuether ◽  
Andrew J. Gangidine ◽  
Sarah Schroeder ◽  
Trinity L. Hamilton
Keyword(s):  
Microbial Community ◽  
Elemental Composition ◽  
Hot Spring ◽  
Siliceous Sinter

scholarly journals Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
María-Eugenia DeCastro ◽  
Michael P. Doane ◽  
Elizabeth Ann Dinsdale ◽  
Esther Rodríguez-Belmonte ◽  
María-Isabel González-Siso
Keyword(s):  
Microbial Community ◽  
Hot Spring ◽  
Functional Characterization ◽  
Tca Cycle ◽  
Maximal Activity ◽  
Sulfur Cycle ◽  
E Coli ◽  
Complex Microbial Community ◽  
Relationship Of ◽  
The Relationship

AbstractIn the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate revealed a complex microbial community dominated by Bacteria in which Proteobacteria and Aquificae were the two prevalent phyla. An association between the two most prevalent genera, Thermus and Hydrogenobacter, was suggested by the relationship of their metabolism. The high relative abundance of sequences involved in the Calvin–Benson cycle and the reductive TCA cycle unveils the dominance of an autotrophic population. Important pathways from the nitrogen and sulfur cycle are potentially taking place in As Burgas hot spring. In the assembled reads, two complete ORFs matching GH2 beta-galactosidases were found. To assess their functional characterization, the two ORFs were cloned and overexpressed in E. coli. The pTsbg enzyme had activity towards o-Nitrophenyl-β-d-galactopyranoside (ONPG) and p-Nitrophenyl-β-d-fucopyranoside, with high thermal stability and showing maximal activity at 85 °C and pH 6, nevertheless the enzyme failed to hydrolyze lactose. The other enzyme, Tsbg, was unable to hydrolyze even ONPG or lactose. This finding highlights the challenge of finding novel active enzymes based only on their sequence.

scholarly journals Accumulation of Elements in Biodeposits on the Stone Surface in Urban Environment. Case Studies from Saint Petersburg, Russia

2020 ◽  
Vol 9 (1) ◽  
pp. 36
Author(s):  
Katerina V. Sazanova (nee Barinova) ◽  
Marina S. Zelenskaya ◽  
Vera V. Manurtdinova ◽  
Alina R. Izatulina ◽  
Aleksei V. Rusakov ◽  
...  
Keyword(s):  
Microbial Community ◽  
Optical Microscopy ◽  
Elemental Composition ◽  
Urban Environment ◽  
Microbial Metabolites ◽  
Stone Surface ◽  
Saint Petersburg ◽  
Icp Ms ◽  
Living Organisms ◽  
Selective Accumulation

The pattern of elements accumulation in biodeposits formed by living organisms and extracellular products of their metabolism (biofouling, primary soils) on different bedrocks (of the monuments of Historical necropoleis in Saint Petersburg) were studied by a complex of biological and mineralogical methods (optical microscopy, SEM, EDX, XRD, ICP MS, XRFS). The content of 46 elements in biodeposits with various communities of microorganisms is determined. The model recreating the picture of the input and selective accumulation of elements in biodeposits on the stone surface in outdoor conditions is assumed. It is shown that the main contribution to the elemental composition of biodeposits is made by the environment and the composition of the microbial community. The contribution of leaching under the action of microbial metabolites of mineral grains, entering biodeposits from the environment, is significantly greater than that of the underlying rock.

scholarly journals Investigation of the microbial community in the Odisha hot spring cluster based on the cultivation independent approach

Genomics Data ◽  
2016 ◽  
Vol 7 ◽  
pp. 222-225 ◽  
Author(s):  
Archana Singh ◽  
Enketeswara Subudhi ◽  
Rajesh Kumar Sahoo ◽  
Mahendra Gaur
Keyword(s):  
Microbial Community ◽  
Hot Spring

scholarly journals Multiscale Microbial Preservation and Biogeochemical Signals in a Modern Hot-Spring Siliceous Sinter Rich in CO2 Emissions, Krýsuvík Geothermal Field, Iceland

Minerals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 263
Author(s):  
Jose Javier Álvaro ◽  
Mónica Sánchez-Román ◽  
Klaas G.J. Nierop ◽  
Francien Peterse
Keyword(s):  
Fatty Acids ◽  
Dissolved Inorganic Carbon ◽  
Hot Spring ◽  
Carbon Isotopic Composition ◽  
Methanogenic Archaea ◽  
Geothermal Field ◽  
Carbon Isotopic ◽  
Siliceous Sinter ◽  
Episodic Fluctuations ◽  
Abandoned Meanders

The microbial communities inferred in silica sinter rocks, based on multiscale morphological features (fabrics and textures) and the presence of lipid biomarkers and their carbon isotopic composition, are evaluated in the Krýsuvík geothermal area of Iceland. Close to vent environments (T > 75 °C and pH 1.7‒3), stream floors are capped with homogeneous vitreous crusts and breccia levels, with no distinct recognizable silicified microbes. About 4 m far from the vents (T 75‒60 °C and pH 3‒6) and beyond (T < 60 °C and pH 6‒7.6), microbial sinters, including wavy and palisade laminated and bubble fabrics, differ between abandoned meanders and desiccated ponds. Fabric and texture variances are related to changes in the ratio of filament/coccoid silicified microbes and associated porosity. Coatings of epicellular silica, less than 2 µm thick, favor identification of individual microbial filaments, whereas coalescence of opal spheres into agglomerates precludes recognition of original microbial textures and silicified microbes. Episodic fluctuations in the physico-chemical conditions of surface waters controlled the acidic hydrolysis of biomarkers. Wavy laminated fabrics from pond margins comprise fatty acids, mono- and dialkyl glycerol, mono- and diethers, monoalkyl glycerol esters and small traces of 10-methyl branched C16 and C18 fatty acids and archaeol, indicative of intergrowths of cyanobacteria, Aquificales, and sulfate reducing bacteria and methanogenic archaea. In contrast, wavy laminated fabrics from abandoned meanders and palisade laminated fabrics from ponds differ in their branched fatty acids and the presence vs. absence of bacteriohopanetetrol, reflecting different cyanobacterial contributions. δ13C values of biomarkers range from −22.7 to −32.9‰, but their values in the wavy (pond) and bubble fabrics have much wider ranges than those of the wavy (meander), palisade, and vitreous fabrics, reflecting dissolved inorganic carbon (DIC) sources and a decrease in 13C downstream outflow channels, with heavier values closer to vents and depleted values in ponds.

Metagenomic microbial community profiling of Unnai hot spring by Ion-Torrent based shotgun sequencing

Microbiology ◽  
2018 ◽  
Vol 87 (1) ◽  
pp. 143-146 ◽  
Author(s):  
A. V. Mangrola ◽  
P. R. Dudhagara ◽  
P. G. Koringa ◽  
C. G. Joshi ◽  
R. K. Patel
Keyword(s):  
Microbial Community ◽  
Hot Spring ◽  
Shotgun Sequencing ◽  
Ion Torrent ◽  
Microbial Community Profiling ◽  
Community Profiling

scholarly journals Isolation of highly thermostable β-xylosidases from a hot spring soil microbial community using a metagenomic approach

DNA Research ◽  
2017 ◽  
Vol 24 (6) ◽  
pp. 649-656 ◽  
Author(s):  
Masaru Sato ◽  
Migiwa Suda ◽  
Jiro Okuma ◽  
Tomohiko Kato ◽  
Yoshitsugu Hirose ◽  
...  
Keyword(s):  
Microbial Community ◽  
Soil Microbial Community ◽  
Hot Spring ◽  
Soil Microbial ◽  
Metagenomic Approach

scholarly journals Merging isotopes and community genomics in a siliceous sinter-depositing hot spring

2011 ◽  
Vol 116 (G1) ◽  
Author(s):  
Jeff R. Havig ◽  
Jason Raymond ◽  
D'Arcy R. Meyer-Dombard ◽  
Natalya Zolotova ◽  
Everett L. Shock
Keyword(s):  
Hot Spring ◽  
Siliceous Sinter

scholarly journals Silicified Microbial Community at Steep Cone Hot Spring, Yellowstone National Park.

2001 ◽  
Vol 16 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Fumio Inagaki ◽  
Yoshinobu Motomura ◽  
Katsumi Doi ◽  
Sachihiro Taguchi ◽  
Eiji Izawa ◽  
...  
Keyword(s):  
Microbial Community ◽  
Yellowstone National Park ◽  
National Park ◽  
Hot Spring
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