scholarly journals Investigation of the microbial community in the Odisha hot spring cluster based on the cultivation independent approach

Genomics Data ◽  
2016 ◽  
Vol 7 ◽  
pp. 222-225 ◽  
Author(s):  
Archana Singh ◽  
Enketeswara Subudhi ◽  
Rajesh Kumar Sahoo ◽  
Mahendra Gaur
Keyword(s):  
Microbial Community ◽  
Hot Spring

scholarly journals Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
María-Eugenia DeCastro ◽  
Michael P. Doane ◽  
Elizabeth Ann Dinsdale ◽  
Esther Rodríguez-Belmonte ◽  
María-Isabel González-Siso
Keyword(s):  
Microbial Community ◽  
Hot Spring ◽  
Functional Characterization ◽  
Tca Cycle ◽  
Maximal Activity ◽  
Sulfur Cycle ◽  
E Coli ◽  
Complex Microbial Community ◽  
Relationship Of ◽  
The Relationship

AbstractIn the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate revealed a complex microbial community dominated by Bacteria in which Proteobacteria and Aquificae were the two prevalent phyla. An association between the two most prevalent genera, Thermus and Hydrogenobacter, was suggested by the relationship of their metabolism. The high relative abundance of sequences involved in the Calvin–Benson cycle and the reductive TCA cycle unveils the dominance of an autotrophic population. Important pathways from the nitrogen and sulfur cycle are potentially taking place in As Burgas hot spring. In the assembled reads, two complete ORFs matching GH2 beta-galactosidases were found. To assess their functional characterization, the two ORFs were cloned and overexpressed in E. coli. The pTsbg enzyme had activity towards o-Nitrophenyl-β-d-galactopyranoside (ONPG) and p-Nitrophenyl-β-d-fucopyranoside, with high thermal stability and showing maximal activity at 85 °C and pH 6, nevertheless the enzyme failed to hydrolyze lactose. The other enzyme, Tsbg, was unable to hydrolyze even ONPG or lactose. This finding highlights the challenge of finding novel active enzymes based only on their sequence.

Metagenomic microbial community profiling of Unnai hot spring by Ion-Torrent based shotgun sequencing

Microbiology ◽  
2018 ◽  
Vol 87 (1) ◽  
pp. 143-146 ◽  
Author(s):  
A. V. Mangrola ◽  
P. R. Dudhagara ◽  
P. G. Koringa ◽  
C. G. Joshi ◽  
R. K. Patel
Keyword(s):  
Microbial Community ◽  
Hot Spring ◽  
Shotgun Sequencing ◽  
Ion Torrent ◽  
Microbial Community Profiling ◽  
Community Profiling

scholarly journals Isolation of highly thermostable β-xylosidases from a hot spring soil microbial community using a metagenomic approach

DNA Research ◽  
2017 ◽  
Vol 24 (6) ◽  
pp. 649-656 ◽  
Author(s):  
Masaru Sato ◽  
Migiwa Suda ◽  
Jiro Okuma ◽  
Tomohiko Kato ◽  
Yoshitsugu Hirose ◽  
...  
Keyword(s):  
Microbial Community ◽  
Soil Microbial Community ◽  
Hot Spring ◽  
Soil Microbial ◽  
Metagenomic Approach

scholarly journals Silicified Microbial Community at Steep Cone Hot Spring, Yellowstone National Park.

2001 ◽  
Vol 16 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Fumio Inagaki ◽  
Yoshinobu Motomura ◽  
Katsumi Doi ◽  
Sachihiro Taguchi ◽  
Eiji Izawa ◽  
...  
Keyword(s):  
Microbial Community ◽  
Yellowstone National Park ◽  
National Park ◽  
Hot Spring

scholarly journals Distribution of Acetothermia-dominated microbial communities in alkaline hot springs of Baikal Rift Zone.

2017 ◽  
Author(s):  
Svetlana V. Zaitseva ◽  
Elena V. Lavrentieva ◽  
Aryuna A. Radnagurueva ◽  
Olga A. Baturina ◽  
Marsel R. Kabilov ◽  
...  
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Microbial Communities ◽  
Hot Springs ◽  
Rift Zone ◽  
Hot Spring ◽  
Baikal Rift Zone ◽  
Rrna Gene ◽  
Acetyl Coa Pathway

Alkaline hot springs are unique extreme habitats resemble the early Earth and present a valuable resource for the discovery of procaryotic community diversity and isolation of the novel thermophilic Bacteria and Archaea. One of the model for the possible origin of biochemistry in alkaline hot springs revealed the acetyl-CoA pathway of CO2 fixation might be the most ancient form of carbon metabolism. Recent phylogenetic studies have suggested that the phylum Acetothermia is one of the deep branches of the Bacteria domain. Firstly Acetothermia (Candidate division OP1) was characterized in a culture independent molecular phylogenetic survey based on the 16S rRNA gene of the sulfide-rich hot spring, Obsidian Pool, a 75 to 95oC hot spring. Two nearly complete genomes of Acetothermia were established based on genome-resolved metagenomic analysis and its capability of implementing acetogenesis through the ancient reductive acetyl-CoA pathway by utilizing CO2 and H2 was revealed. Although genomic, proteomic and metagenomic approaches investigate basic metabolism and potentional energy conservation of uncultivated candidate phyla but ecological roles of these bacteria and general patterns of diversity and community structure stay unclear. General hydrochemical and geological characterization of alkaline thermal springs of the Baikal Rift zone with high silica concentrations and a nitrogen dominated gas phase is provided. Previous microbiogical studies based on culture-dependent methods recovered a large number of bacterial strains from thermal springs located in Baikal Rift zone. We combined microbial communities analysis by using high-throughput 16S rRNA gene sequencing, biogeochemical measurements, sediment mineralogy and physicochemical characteristics to investigate ecosystems of alkaline hot springs located in the Baikal Rift zone. Uncultivated bacteria belonging to the phylum Acetothermia, along with members of the phyla Firmicutes and Proteobacteria, were identified as the dominant group in hydrothermal sediments communities in the alkaline hot springs of Baikal Rift zone. In bottom sediments of the Alla hot spring, about 57% of all classified sequences represent this phylum. Geochemistry of fluids and sample type were strongly correlated with microbial community composition. The Acetothermia exhibited the highest relative abundance in sediment microbial community associated with alkaline thermal fluids enriched in Fe, Zn, Ni, Al and Cr.

scholarly journals Stable Isotope Probing for Microbial Iron Reduction in Chocolate Pots Hot Spring, Yellowstone National Park

2018 ◽  
Vol 84 (11) ◽  
Author(s):  
Nathaniel W. Fortney ◽  
Shaomei He ◽  
Ajinkya Kulkarni ◽  
Michael W. Friedrich ◽  
Charlotte Holz ◽  
...  
Keyword(s):  
Microbial Community ◽  
Stable Isotope ◽  
Yellowstone National Park ◽  
National Park ◽  
Hot Spring ◽  
Redox Cycling ◽  
Stable Isotope Probing ◽  
Content Type ◽  
Insight Into

ABSTRACTChocolate Pots hot springs (CP) is a circumneutral-pH Fe-rich geothermal feature located in Yellowstone National Park. Previous Fe(III)-reducing enrichment culture studies with CP sediments identified close relatives of known dissimilatory Fe(III)-reducing bacterial (FeRB) taxa, includingGeobacterandMelioribacter. However, the abundances and activities of such organisms in the native microbial community are unknown. Here, we used stable isotope probing experiments combined with 16S rRNA gene amplicon and shotgun metagenomic sequencing to gain an understanding of thein situFe(III)-reducing microbial community at CP. Fe-Si oxide precipitates collected near the hot spring vent were incubated with unlabeled and13C-labeled acetate to target active FeRB. We searched reconstructed genomes for homologs of genes involved in known extracellular electron transfer (EET) systems to identify the taxa involved in Fe redox transformations. Known FeRB taxa containing putative EET systems (Geobacter,Ignavibacteria) increased in abundance under acetate-amended conditions, whereas genomes related toIgnavibacteriumandThermodesulfovibriothat contained putative EET systems were recovered from incubations without electron donor. Our results suggest that FeRB play an active role in Fe redox cycling within Fe-Si oxide-rich deposits located at the hot spring vent.IMPORTANCEThe identification of past near-surface hydrothermal environments on Mars emphasizes the importance of using modern Earth environments, such as CP, to gain insight into potential Fe-based microbial life on other rocky worlds, as well as ancient Fe-rich Earth ecosystems. By combining stable carbon isotope probing techniques and DNA sequencing technology, we gained insight into the pathways of microbial Fe redox cycling at CP. The results suggest that microbial Fe(III) oxide reduction is prominentin situ, with important implications for the generation of geochemical and stable Fe isotopic signatures of microbial Fe redox metabolism within Fe-rich circumneutral-pH thermal spring environments on Earth and Mars.

scholarly journals Microbial biodiversity of thermal water and mud in an Italian spa by metagenomics: a pilot study

2017 ◽  
Vol 18 (4) ◽  
pp. 1456-1465 ◽  
Author(s):  
Stefania Paduano ◽  
Federica Valeriani ◽  
Vincenzo Romano-Spica ◽  
Annalisa Bargellini ◽  
Paola Borella ◽  
...  
Keyword(s):  
Microbial Community ◽  
Water Distribution ◽  
Thermal Water ◽  
Hot Spring ◽  
Water Distribution Network ◽  
Rrna Gene ◽  
Sequences Analysis ◽  
Next Generation Sequencing Ngs ◽  
First Time ◽  
Generation Sequencing

Abstract In literature, the microbial diversity of hot spring waters has been the focus of extensive research, while there is a paucity of studies on thermal water distribution network of spa centres and, as yet, no studies have been carried out on the bacterial population of thermal muds. In this context, the aim of our study is to characterize the microbial community of sulphurous-bromine-iodine thermal water and mud within an Italian spa complex using Next Generation Sequencing (NGS) technologies. This is the first report of microbiome characterization along a water supply network from the spring to points of use within a spa. According to the 16S rRNA gene sequences analysis, our data highlight the presence of a typical microbial community, mainly composed of sulphur-cycling bacteria belonging to Desulfomonile, Thermodesulfovibrio, Geothermobacterium, Thermus, Thiofaba and Syntrophomonas genera. In addition, the characterization and evolution of the bacterial community in thermal muds during the maturation process is investigated for the first time. Interestingly, the microbiome of mature mud resulted dominated by bacteria capable of lipid biosynthesis, suggesting that these bacteria may play a role in the anti-rheumatic properties of thermal mud.

Complete Nitrogen Cycle Driven by the Thermophilic Microbial Community of Rupi II Hot Spring

2021 ◽  
Author(s):  
Nikoleta Boteva ◽  
Nils-Kåre Birkeland ◽  
Margarita Kambourova
Keyword(s):  
Microbial Community ◽  
Nitrogen Cycle ◽  
Hot Spring
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