scholarly journals Silicified Microbial Community at Steep Cone Hot Spring, Yellowstone National Park.

2001 ◽  
Vol 16 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Fumio Inagaki ◽  
Yoshinobu Motomura ◽  
Katsumi Doi ◽  
Sachihiro Taguchi ◽  
Eiji Izawa ◽  
...  
Keyword(s):  
Microbial Community ◽  
Yellowstone National Park ◽  
National Park ◽  
Hot Spring

scholarly journals Stable Isotope Probing for Microbial Iron Reduction in Chocolate Pots Hot Spring, Yellowstone National Park

2018 ◽  
Vol 84 (11) ◽  
Author(s):  
Nathaniel W. Fortney ◽  
Shaomei He ◽  
Ajinkya Kulkarni ◽  
Michael W. Friedrich ◽  
Charlotte Holz ◽  
...  
Keyword(s):  
Microbial Community ◽  
Stable Isotope ◽  
Yellowstone National Park ◽  
National Park ◽  
Hot Spring ◽  
Redox Cycling ◽  
Stable Isotope Probing ◽  
Content Type ◽  
Insight Into

ABSTRACTChocolate Pots hot springs (CP) is a circumneutral-pH Fe-rich geothermal feature located in Yellowstone National Park. Previous Fe(III)-reducing enrichment culture studies with CP sediments identified close relatives of known dissimilatory Fe(III)-reducing bacterial (FeRB) taxa, includingGeobacterandMelioribacter. However, the abundances and activities of such organisms in the native microbial community are unknown. Here, we used stable isotope probing experiments combined with 16S rRNA gene amplicon and shotgun metagenomic sequencing to gain an understanding of thein situFe(III)-reducing microbial community at CP. Fe-Si oxide precipitates collected near the hot spring vent were incubated with unlabeled and13C-labeled acetate to target active FeRB. We searched reconstructed genomes for homologs of genes involved in known extracellular electron transfer (EET) systems to identify the taxa involved in Fe redox transformations. Known FeRB taxa containing putative EET systems (Geobacter,Ignavibacteria) increased in abundance under acetate-amended conditions, whereas genomes related toIgnavibacteriumandThermodesulfovibriothat contained putative EET systems were recovered from incubations without electron donor. Our results suggest that FeRB play an active role in Fe redox cycling within Fe-Si oxide-rich deposits located at the hot spring vent.IMPORTANCEThe identification of past near-surface hydrothermal environments on Mars emphasizes the importance of using modern Earth environments, such as CP, to gain insight into potential Fe-based microbial life on other rocky worlds, as well as ancient Fe-rich Earth ecosystems. By combining stable carbon isotope probing techniques and DNA sequencing technology, we gained insight into the pathways of microbial Fe redox cycling at CP. The results suggest that microbial Fe(III) oxide reduction is prominentin situ, with important implications for the generation of geochemical and stable Fe isotopic signatures of microbial Fe redox metabolism within Fe-rich circumneutral-pH thermal spring environments on Earth and Mars.

scholarly journals Pyrobaculum yellowstonensis Strain WP30 Respires on Elemental Sulfur and/or Arsenate in Circumneutral Sulfidic Geothermal Sediments of Yellowstone National Park

2015 ◽  
Vol 81 (17) ◽  
pp. 5907-5916 ◽  
Author(s):  
Z. J. Jay ◽  
J. P. Beam ◽  
A. Dohnalkova ◽  
R. Lohmayer ◽  
B. Bodle ◽  
...  
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Elemental Sulfur ◽  
De Novo ◽  
Hot Spring ◽  
Content Type ◽  
Physiological Attributes ◽  
And Function ◽  
Metagenome Sequence

ABSTRACTThermoproteales(phylumCrenarchaeota) populations are abundant in high-temperature (>70°C) environments of Yellowstone National Park (YNP) and are important in mediating the biogeochemical cycles of sulfur, arsenic, and carbon. The objectives of this study were to determine the specific physiological attributes of the isolatePyrobaculum yellowstonensisstrain WP30, which was obtained from an elemental sulfur sediment (Joseph's Coat Hot Spring [JCHS], 80°C, pH 6.1, 135 μM As) and relate this organism to geochemical processes occurringin situ. Strain WP30 is a chemoorganoheterotroph and requires elemental sulfur and/or arsenate as an electron acceptor. Growth in the presence of elemental sulfur and arsenate resulted in the formation of thioarsenates and polysulfides. The complete genome of this organism was sequenced (1.99 Mb, 58% G+C content), revealing numerous metabolic pathways for the degradation of carbohydrates, amino acids, and lipids. Multiple dimethyl sulfoxide-molybdopterin (DMSO-MPT) oxidoreductase genes, which are implicated in the reduction of sulfur and arsenic, were identified. Pathways for thede novosynthesis of nearly all required cofactors and metabolites were identified. The comparative genomics ofP. yellowstonensisand the assembled metagenome sequence from JCHS showed that this organism is highly related (∼95% average nucleotide sequence identity) toin situpopulations. The physiological attributes and metabolic capabilities ofP. yellowstonensisprovide an important foundation for developing an understanding of the distribution and function of these populations in YNP.

scholarly journals Temporal and Seasonal Variations of the Hot Spring Basin Hydrothermal System, Yellowstone National Park, USA

2013 ◽  
Vol 5 (12) ◽  
pp. 6587-6610 ◽  
Author(s):  
Cheryl Jaworowski ◽  
Henry Heasler ◽  
Christopher Neale ◽  
Sivarajan Saravanan ◽  
Ashish Masih
Keyword(s):  
Seasonal Variations ◽  
Hydrothermal System ◽  
Yellowstone National Park ◽  
National Park ◽  
Hot Spring

scholarly journals Whole-Genome Sequence of a Unique Elioraea Species Strain Isolated from a Yellowstone National Park Hot Spring

2019 ◽  
Vol 8 (44) ◽  
Author(s):  
Sydney Robertson ◽  
Robert F. Ramaley ◽  
Terry Meyer ◽  
John A. Kyndt
Keyword(s):  
New Species ◽  
Genome Sequence ◽  
Yellowstone National Park ◽  
National Park ◽  
Photosynthetic Bacteria ◽  
Hot Spring ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Anoxygenic Photosynthetic Bacteria

The genus Elioraea has only one species characterized microbiologically and two genomes sequenced. We have sequenced the genome of a unique Elioraea strain isolated from Yellowstone National Park and found it to be a distinct new species. Elioraea is suggested to be a member of the aerobic anoxygenic photosynthetic bacteria.

scholarly journals Microbial Fe(III) oxide reduction potential in Chocolate Pots hot spring, Yellowstone National Park

Geobiology ◽  
2016 ◽  
Vol 14 (3) ◽  
pp. 255-275 ◽  
Author(s):  
N. W. Fortney ◽  
S. He ◽  
B. J. Converse ◽  
B. L. Beard ◽  
C. M. Johnson ◽  
...  
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Reduction Potential ◽  
Hot Spring ◽  
Oxide Reduction

scholarly journals Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment.

1994 ◽  
Vol 91 (5) ◽  
pp. 1609-1613 ◽  
Author(s):  
S. M. Barns ◽  
R. E. Fundyga ◽  
M. W. Jeffries ◽  
N. R. Pace
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Hot Spring ◽  
Archaeal Diversity
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