3-Mercaptopyruvate Sulfurtransferase Produces Hydrogen Sulfide and Bound Sulfane Sulfur in the Brain

2009 ◽  
Vol 11 (4) ◽  
pp. 703-714 ◽  
Author(s):  
Norihiro Shibuya ◽  
Makiko Tanaka ◽  
Mikiharu Yoshida ◽  
Yuki Ogasawara ◽  
Tadayasu Togawa ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Sulfane Sulfur ◽  
Mercaptopyruvate Sulfurtransferase ◽  
The Brain

3-Mercaptopyruvate sulfurtransferase produces hydrogen sulfide that is stored as bound sulfane sulfur in the brain

2009 ◽  
Vol 65 ◽  
pp. S57 ◽  
Author(s):  
Norihiro Shibuya ◽  
Makiko Tanaka ◽  
Mikiharu Yoshida ◽  
Yuki Ogasawara ◽  
Tadayasu Togawa ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Sulfane Sulfur ◽  
Mercaptopyruvate Sulfurtransferase ◽  
The Brain

scholarly journals Hydrogen Sulfide (H2S) and Polysulfide (H2Sn) Signaling: The First 25 Years

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 896
Author(s):  
Hideo Kimura
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Hydrogen Sulfide ◽  
Research Area ◽  
Cytoprotective Effect ◽  
Mercaptopyruvate Sulfurtransferase ◽  
Energy Formation ◽  
New Research ◽  
The Brain ◽  
Activity Energy

Since the first description of hydrogen sulfide (H2S) as a toxic gas in 1713 by Bernardino Ramazzini, most studies on H2S have concentrated on its toxicity. In 1989, Warenycia et al. demonstrated the existence of endogenous H2S in the brain, suggesting that H2S may have physiological roles. In 1996, we demonstrated that hydrogen sulfide (H2S) is a potential signaling molecule, which can be produced by cystathionine β-synthase (CBS) to modify neurotransmission in the brain. Subsequently, we showed that H2S relaxes vascular smooth muscle in synergy with nitric oxide (NO) and that cystathionine γ-lyase (CSE) is another producing enzyme. This study also opened up a new research area of a crosstalk between H2S and NO. The cytoprotective effect, anti-inflammatory activity, energy formation, and oxygen sensing by H2S have been subsequently demonstrated. Two additional pathways for the production of H2S with 3-mercaptopyruvate sulfurtransferase (3MST) from l- and d-cysteine have been identified. We also discovered that hydrogen polysulfides (H2Sn, n ≥ 2) are potential signaling molecules produced by 3MST. H2Sn regulate the activity of ion channels and enzymes, as well as even the growth of tumors. S-Sulfuration (S-sulfhydration) proposed by Snyder is the main mechanism for H2S/H2Sn underlying regulation of the activity of target proteins. This mini review focuses on the key findings on H2S/H2Sn signaling during the first 25 years.

3-Mercaptopyruvate sulfurtransferase: an enzyme at the crossroads of sulfane sulfur trafficking

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Brandán Pedre ◽  
Tobias P. Dick
Keyword(s):  
Oxidative Stress ◽  
Metabolic Disease ◽  
Hydrogen Sulfide ◽  
Fatty Acid ◽  
Stress Resistance ◽  
Respiratory Function ◽  
Acid Metabolism ◽  
Cyanide Detoxification ◽  
Mercaptopyruvate Sulfurtransferase ◽  
Sulfur Trafficking

Abstract3-Mercaptopyruvate sulfurtransferase (MPST) catalyzes the desulfuration of 3-mercaptopyruvate to generate an enzyme-bound hydropersulfide. Subsequently, MPST transfers the persulfide’s outer sulfur atom to proteins or small molecule acceptors. MPST activity is known to be involved in hydrogen sulfide generation, tRNA thiolation, protein urmylation and cyanide detoxification. Tissue-specific changes in MPST expression correlate with ageing and the development of metabolic disease. Deletion and overexpression experiments suggest that MPST contributes to oxidative stress resistance, mitochondrial respiratory function and the regulation of fatty acid metabolism. However, the role and regulation of MPST in the larger physiological context remain to be understood.

scholarly journals A Hypothesis: Hydrogen Sulfide Might Be Neuroprotective against Subarachnoid Hemorrhage Induced Brain Injury

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Yong-Peng Yu ◽  
Xiang-Lin Chi ◽  
Li-Jun Liu
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Hydrogen Sulfide ◽  
Subarachnoid Hemorrhage ◽  
Brain Injury ◽  
Inflammatory Responses ◽  
Ischemia Reperfusion Injury ◽  
Leukocyte Adhesion ◽  
Ischemia Reperfusion ◽  
The Brain

Gases such as nitric oxide (NO) and carbon monoxide (CO) play important roles both in normal physiology and in disease. Recent studies have shown that hydrogen sulfide (H2S) protects neurons against oxidative stress and ischemia-reperfusion injury and attenuates lipopolysaccharides (LPS) induced neuroinflammation in microglia, exhibiting anti-inflammatory and antiapoptotic activities. The gas H2S is emerging as a novel regulator of important physiologic functions such as arterial diameter, blood flow, and leukocyte adhesion. It has been known that multiple factors, including oxidative stress, free radicals, and neuronal nitric oxide synthesis as well as abnormal inflammatory responses, are involved in the mechanism underlying the brain injury after subarachnoid hemorrhage (SAH). Based on the multiple physiologic functions of H2S, we speculate that it might be a promising, effective, and specific therapy for brain injury after SAH.

scholarly journals A Source of Hydrogen Sulfide and a Mechanism of Its Release in the Brain

2009 ◽  
Vol 11 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Mari Ishigami ◽  
Keiko Hiraki ◽  
Ken Umemura ◽  
Yuki Ogasawara ◽  
Kazuyuki Ishii ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
The Brain

scholarly journals Menadione effect on l-cysteine desulfuration in U373 cells.

2007 ◽  
Vol 54 (2) ◽  
pp. 407-411 ◽  
Author(s):  
Maria Wróbel ◽  
Halina Jurkowska
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Control Culture ◽  
Oxygen Species ◽  
Active Centers ◽  
Glutathione Synthesis ◽  
Sulfane Sulfur ◽  
Mercaptopyruvate Sulfurtransferase ◽  
Sulfur Level ◽  
U373 Cells

The non-cytotoxic concentration (20 microM) of menadione (2-methyl-1,4-naphthoquinone), after 1 h of incubation, leads to loss of the activity of rhodanese by 33%, 3-mercaptopyruvate sulfurtransferase by 20%, as well as the level of sulfane sulfur by about 23% and glutathione by 12%, in the culture of U373 cells, in comparison with the control culture. Reactive oxygen species generated by menadione oxidize sulfhydryl groups in active centers of the investigated enzymes, inhibiting them and saving cysteine for glutathione synthesis. A decreased sulfane sulfur level can be correlated with an oxidative stress.

scholarly journals Effect of S-Allyl –L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis

2020 ◽  
Vol 21 (3) ◽  
pp. 1090 ◽  
Author(s):  
Patrycja Bronowicka-Adamska ◽  
Anna Bentke ◽  
Małgorzata Lasota ◽  
Maria Wróbel
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Breast Adenocarcinoma ◽  
Sulfane Sulfur ◽  
Mercaptopyruvate Sulfurtransferase ◽  
Late Apoptosis ◽  
Sulfur Level ◽  
H2s Production ◽  
Level Reduction ◽  
Mcf 7

The S-Allyl-L-cysteine (SAC) component of aged garlic extract (AGE) is proven to have anticancer, antihepatotoxic, neuroprotective and neurotrophic properties. γ-Cystathionase (CTH), cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (MPST) are involved in H2S/sulfane sulfur endogenous formation from L-cysteine. The aim of the study was to determine the effect of SAC on MCF-7 cells survival and apoptosis, which is a widely known approach to reduce the number of cancer cells. An additional goal of this paper was to investigate the effect of SAC on the activity and expression of enzymes involved in H2S production. The experiments were carried out in the human breast adenocarcinoma cell line MCF-7. Changes in the cell viability were determined by MTT assay. Cell survival was determined by flow cytometry (FC). Changes in enzymes expression were analyzed using Western blot. After 24 h and 48 h incubation with 2245 µM SAC, induction of late apoptosis was observed. A decrease in cell viability was observed with increasing SAC concentration and incubation time. SAC had no significant cytotoxic effect on the MCF-7 cells upon all analyzed concentrations. CTH, MPST and CBS expression were confirmed in non-treated MCF-7 cells. Significant decrease in MPST activity at 2245 µM SAC after 24 h and 48 h incubation vs. 1000 µM SAC was associated with decrease in sulfane sulfur levels. The presented results show promising SAC effects regarding the deterioration of the MCF-7 cells’ condition in reducing their viability through the downregulation of MPST expression and sulfate sulfur level reduction.

Hydrogen Sulfide Increases Endothelial Permeability by Bound Sulfane Sulfur

2014 ◽  
Vol 76 ◽  
pp. S56
Author(s):  
Shuai Yuan ◽  
Arif Yurdagul ◽  
Sibile Pardue ◽  
A. Wayne Orr ◽  
Christopher G. Kevil
Keyword(s):  
Hydrogen Sulfide ◽  
Endothelial Permeability ◽  
Sulfane Sulfur

scholarly journals Lipoic Acid as a Possible Pharmacological Source of Hydrogen Sulfide/Sulfane Sulfur

Molecules ◽  
2017 ◽  
Vol 22 (3) ◽  
pp. 388 ◽  
Author(s):  
Anna Bilska-Wilkosz ◽  
Małgorzata Iciek ◽  
Danuta Kowalczyk-Pachel ◽  
Magdalena Górny ◽  
Maria Sokołowska-Jeżewicz ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Lipoic Acid ◽  
Sulfane Sulfur
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