Overproduction of Erythromycin by Ultraviolet Mutagenesis and Expression of ermE Gene in Saccharopolyspora erythraea

2017 ◽  
Vol 15 (7) ◽  
pp. 314-319 ◽  
Author(s):  
Nargis Fallahpour ◽  
Sanam Adnani ◽  
Hossein Rassi ◽  
Esmaeil Asli
Keyword(s):  
Saccharopolyspora Erythraea ◽  
Ultraviolet Mutagenesis

Joint engineering of SACE_Lrp and its target MarR enhances the biosynthesis and export of erythromycin in Saccharopolyspora erythraea

2021 ◽  
Vol 105 (7) ◽  
pp. 2911-2924
Author(s):  
Jing Liu ◽  
Long Li ◽  
Yunxia Wang ◽  
Bowen Li ◽  
Xinlu Cai ◽  
...  
Keyword(s):  
Saccharopolyspora Erythraea

In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea

2015 ◽  
Vol 100 (5) ◽  
pp. 2257-2266 ◽  
Author(s):  
Hang Wu ◽  
Weiwei Li ◽  
Chen Xin ◽  
Congming Zhang ◽  
Yansheng Wang ◽  
...  
Keyword(s):  
Saccharopolyspora Erythraea

New tenvermectin analogs obtained by microbial conversion with Saccharopolyspora erythraea

2016 ◽  
Vol 70 (2) ◽  
pp. 190-192 ◽  
Author(s):  
Xu Wan ◽  
Shao-yong Zhang ◽  
Hui Zhang ◽  
Jun Zhai ◽  
Jun Huang ◽  
...  
Keyword(s):  
Saccharopolyspora Erythraea ◽  
Microbial Conversion

scholarly journals Regulation of Sporangium Formation by BldD in the Rare Actinomycete Actinoplanes missouriensis

2017 ◽  
Vol 199 (12) ◽  
Author(s):  
Yoshihiro Mouri ◽  
Kenji Konishi ◽  
Azusa Fujita ◽  
Takeaki Tezuka ◽  
Yasuo Ohnishi
Keyword(s):  
Vegetative Growth ◽  
Streptomyces Coelicolor ◽  
Morphological Differentiation ◽  
Transcriptional Regulator ◽  
Saccharopolyspora Erythraea ◽  
Transcriptional Analysis ◽  
Morphological Development ◽  
Sequencing Analysis ◽  
Content Type ◽  
Biological Studies

ABSTRACT The rare actinomycete Actinoplanes missouriensis forms sporangia, including hundreds of flagellated spores that start swimming as zoospores after their release. Under conditions suitable for vegetative growth, zoospores stop swimming and germinate. A comparative proteome analysis between zoospores and germinating cells identified 15 proteins that were produced in larger amounts in germinating cells. They include an orthologue of BldD (herein named AmBldD [BldD of A. missouriensis]), which is a transcriptional regulator involved in morphological development and secondary metabolism in Streptomyces. AmBldD was detected in mycelia during vegetative growth but was barely detected in mycelia during the sporangium-forming phase, in spite of the constant transcription of AmbldD throughout growth. An AmbldD mutant started to form sporangia much earlier than the wild-type strain, and the resulting sporangia were morphologically abnormal. Recombinant AmBldD bound a palindromic sequence, the AmBldD box, located upstream from AmbldD. 3′,5′-Cyclic di-GMP significantly enhanced the in vitro DNA-binding ability of AmBldD. A chromatin immunoprecipitation-sequencing analysis and an in silico search for AmBldD boxes revealed that AmBldD bound 346 genomic loci that contained the 19-bp inverted repeat 5′-NN(G/A)TNACN(C/G)N(G/C)NGTNA(C/T)NN-3′ as the consensus AmBldD-binding sequence. The transcriptional analysis of 27 selected AmBldD target gene candidates indicated that AmBldD should repress 12 of the 27 genes, including bldM, ssgB, whiD, ddbA, and wblA orthologues. These genes are involved in morphological development in Streptomyces coelicolor A3(2). Thus, AmBldD is a global transcriptional regulator that seems to repress the transcription of tens of genes during vegetative growth, some of which are likely to be required for sporangium formation. IMPORTANCE The rare actinomycete Actinoplanes missouriensis undergoes complex morphological differentiation, including sporangium formation. However, almost no molecular biological studies have been conducted on this bacterium. BldD is a key global regulator involved in the morphological development of streptomycetes. BldD orthologues are highly conserved among sporulating actinomycetes, but no BldD orthologues, except one in Saccharopolyspora erythraea, have been studied outside the streptomycetes. Here, it was revealed that the BldD orthologue AmBldD is essential for normal developmental processes in A. missouriensis. The AmBldD regulon seems to be different from the BldD regulon in Streptomyces coelicolor A3(2), but they share four genes that are involved in morphological differentiation in S. coelicolor A3(2).

scholarly journals Isolation and Characterization of 7-Hydroxy-6-demethyl-6-deoxy-erythromycin D, a New Erythromycin Analogue, from Engineered Saccharopolyspora erythraea.

2004 ◽  
Vol 57 (1) ◽  
pp. 64-67 ◽  
Author(s):  
COURTNEY M. STARKS ◽  
EDUARDO RODRIGUEZ ◽  
JOHN R. CARNEY ◽  
RUCHIR P. DESAI ◽  
CHRIS CARRERAS ◽  
...  
Keyword(s):  
Saccharopolyspora Erythraea ◽  
Isolation And Characterization
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