Macrolide antibiotic biosynthesis: isolation and properties of two forms of 6-deoxyerythronolide B hydroxylase from Saccharopolyspora erythraea (Streptomyces erythreus)

Biochemistry ◽  
1987 ◽  
Vol 26 (19) ◽  
pp. 6204-6210 ◽  
Author(s):  
Ali Shafiee ◽  
C. Richard Hutchinson
Keyword(s):  
Macrolide Antibiotic ◽  
Saccharopolyspora Erythraea ◽  
Antibiotic Biosynthesis

Polyene Macrolide Antibiotic Biosynthesis

2004 ◽  
Vol 11 (12) ◽  
pp. 1643-1656 ◽  
Author(s):  
J. Aparicio ◽  
M. Mendes ◽  
N. Anton ◽  
E. Recio ◽  
J. Martin
Keyword(s):  
Macrolide Antibiotic ◽  
Antibiotic Biosynthesis ◽  
Polyene Macrolide ◽  
Polyene Macrolide Antibiotic

Global evolution of glycosylated polyene macrolide antibiotic biosynthesis

2018 ◽  
Vol 127 ◽  
pp. 239-247
Author(s):  
Dongmei Liang ◽  
Xiaoyu Yang ◽  
Jiaheng Liu ◽  
Qinggele Caiyin ◽  
Guangrong Zhao ◽  
...  
Keyword(s):  
Macrolide Antibiotic ◽  
Antibiotic Biosynthesis ◽  
Polyene Macrolide ◽  
Global Evolution ◽  
Polyene Macrolide Antibiotic

Ultrastructural autoradiography of L6 skeletal-muscle cells exposed to a tritiated macrolide antibiotic (LY237216) in vitro

1992 ◽  
Vol 50 (1) ◽  
pp. 612-613
Author(s):  
S.L. White ◽  
C.B. Jensen ◽  
D.D. Giera ◽  
D.A. Laska ◽  
M.N. Novilla ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Quantitative Analysis ◽  
Macrolide Antibiotic ◽  
Circle Method ◽  
Apical Surface ◽  
Polycarbonate Membrane ◽  
L6 Cells ◽  
Low Viscosity ◽  
Erythromycin A

In vitro exposure to LY237216 (9-Deoxo-11-deoxy-9,11-{imino[2-(2-methoxyethoxy)ethylidene]-oxy}-(9S)-erythromycin), a macrolide antibiotic, was found to induce cytoplasmic vacuolation in L6 skeletal muscle myoblast cultures (White, S.L., unpubl). The present study was done to determine, by autoradiographic quantitative analysis, the subcellular distribution of 3H-LY237216 in L6 cells.L6 cells (ATCC, CRL 1458) were cultured to confluency on polycarbonate membrane filters (Millipore Corp., Bedford, MA) in M-199 medium (GIBCO® Labs) with 10% fetal bovine serum. The cells were exposed from the apical surface for 1-hour to unlabelled-compound (0 μCi/ml) or 50 (μCi/ml of 3H-LY237216 at a compound concentration of 0.25 mg/ml. Following a rapid rinse in compound-free growth medium, the cells were slam-frozen against a liquid nitrogen cooled, polished copper block in a CF-100 cryofixation unit (LifeCell Corp., The Woodlands, TX). Specimens were dried in the MDD-C Molecular Distillation Drier (LifeCell Corp.), vapor osmicated and embedded in Spurrs low viscosity resin. Ultrathin sections collected on formvar coated stainless steel grids were counter-stained, then individually mounted on corks. A monolayer of Ilford L4 nuclear emulsion (Polysciences, Inc., Warrington, PA) was placed on the sections, utilizing a modified “loop method”. The emulsions were exposed for 7-weeks in a light-tight box at 4°C. Autoradiographs were developed in Microdol-X developer and examined on a Philips EM410LS transmission electron microscope. Quantitative analysis of compound localization employed the point and circle approach of Williams; incorporating the probability circle method of Salpeter and McHenry.

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