New tenvermectin analogs obtained by microbial conversion with Saccharopolyspora erythraea

2016 ◽  
Vol 70 (2) ◽  
pp. 190-192 ◽  
Author(s):  
Xu Wan ◽  
Shao-yong Zhang ◽  
Hui Zhang ◽  
Jun Zhai ◽  
Jun Huang ◽  
...  
Keyword(s):  
Saccharopolyspora Erythraea ◽  
Microbial Conversion

scholarly journals Microbial conversion of avermectins by Saccharopolyspora erythraea: Hydroxylation at C-27.

1994 ◽  
Vol 47 (3) ◽  
pp. 372-375 ◽  
Author(s):  
MARVIN SCHULMAN ◽  
PATRICK DOHERTY ◽  
DEBORAH ZINK ◽  
BYRON ARISON
Keyword(s):  
Saccharopolyspora Erythraea ◽  
Microbial Conversion

scholarly journals Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
James Kirby ◽  
Gina M. Geiselman ◽  
Junko Yaegashi ◽  
Joonhoon Kim ◽  
Xun Zhuang ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Copy Number ◽  
Mevalonate Pathway ◽  
Mevalonate Kinase ◽  
Metabolite Analysis ◽  
Lignocellulosic Hydrolysate ◽  
Microbial Conversion ◽  
Lignocellulosic Hydrolysates ◽  
Lignocellulosic Feedstocks ◽  
Fuels And Chemicals

Abstract Background Mitigation of climate change requires that new routes for the production of fuels and chemicals be as oil-independent as possible. The microbial conversion of lignocellulosic feedstocks into terpene-based biofuels and bioproducts represents one such route. This work builds upon previous demonstrations that the single-celled carotenogenic basidiomycete, Rhodosporidium toruloides, is a promising host for the production of terpenes from lignocellulosic hydrolysates. Results This study focuses on the optimization of production of the monoterpene 1,8-cineole and the sesquiterpene α-bisabolene in R. toruloides. The α-bisabolene titer attained in R. toruloides was found to be proportional to the copy number of the bisabolene synthase (BIS) expression cassette, which in turn influenced the expression level of several native mevalonate pathway genes. The addition of more copies of BIS under a stronger promoter resulted in production of α-bisabolene at 2.2 g/L from lignocellulosic hydrolysate in a 2-L fermenter. Production of 1,8-cineole was found to be limited by availability of the precursor geranylgeranyl pyrophosphate (GPP) and expression of an appropriate GPP synthase increased the monoterpene titer fourfold to 143 mg/L at bench scale. Targeted mevalonate pathway metabolite analysis suggested that 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGR), mevalonate kinase (MK) and phosphomevalonate kinase (PMK) may be pathway bottlenecks are were therefore selected as targets for overexpression. Expression of HMGR, MK, and PMK orthologs and growth in an optimized lignocellulosic hydrolysate medium increased the 1,8-cineole titer an additional tenfold to 1.4 g/L. Expression of the same mevalonate pathway genes did not have as large an impact on α-bisabolene production, although the final titer was higher at 2.6 g/L. Furthermore, mevalonate pathway intermediates accumulated in the mevalonate-engineered strains, suggesting room for further improvement. Conclusions This work brings R. toruloides closer to being able to make industrially relevant quantities of terpene from lignocellulosic biomass.

Joint engineering of SACE_Lrp and its target MarR enhances the biosynthesis and export of erythromycin in Saccharopolyspora erythraea

2021 ◽  
Vol 105 (7) ◽  
pp. 2911-2924
Author(s):  
Jing Liu ◽  
Long Li ◽  
Yunxia Wang ◽  
Bowen Li ◽  
Xinlu Cai ◽  
...  
Keyword(s):  
Saccharopolyspora Erythraea

A novel strategy for microbial conversion of dairy wastewater into biofertilizer

2021 ◽  
Vol 293 ◽  
pp. 126051
Author(s):  
Mandakini Gogoi ◽  
Tethi Biswas ◽  
Prasandeep Biswal ◽  
Tuhin Saha ◽  
Ajoy Modak ◽  
...  
Keyword(s):  
Dairy Wastewater ◽  
Microbial Conversion ◽  
Novel Strategy

scholarly journals Methanogens: pushing the boundaries of biology

2018 ◽  
Vol 2 (4) ◽  
pp. 629-646 ◽  
Author(s):  
Nicole R. Buan
Keyword(s):  
Energy Conservation ◽  
Trophic Interactions ◽  
Theoretical Basis ◽  
High Energy ◽  
Microbial Conversion ◽  
Microbial Physiology ◽  
Methane Gas ◽  
Biogenic Carbon ◽  
Environmental Microbes

Methanogens are anaerobic archaea that grow by producing methane gas. These microbes and their exotic metabolism have inspired decades of microbial physiology research that continues to push the boundary of what we know about how microbes conserve energy to grow. The study of methanogens has helped to elucidate the thermodynamic and bioenergetics basis of life, contributed our understanding of evolution and biodiversity, and has garnered an appreciation for the societal utility of studying trophic interactions between environmental microbes, as methanogens are important in microbial conversion of biogenic carbon into methane, a high-energy fuel. This review discusses the theoretical basis for energy conservation by methanogens and identifies gaps in methanogen biology that may be filled by undiscovered or yet-to-be engineered organisms.

In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea

2015 ◽  
Vol 100 (5) ◽  
pp. 2257-2266 ◽  
Author(s):  
Hang Wu ◽  
Weiwei Li ◽  
Chen Xin ◽  
Congming Zhang ◽  
Yansheng Wang ◽  
...  
Keyword(s):  
Saccharopolyspora Erythraea

Microbial conversion of biomass into bio-based polymers

2017 ◽  
Vol 245 ◽  
pp. 1664-1673 ◽  
Author(s):  
Hideo Kawaguchi ◽  
Chiaki Ogino ◽  
Akihiko Kondo
Keyword(s):  
Microbial Conversion
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