A Three-Dimensional Lymphatic Endothelial Cell Tube Formation Assay to Identify Novel Kinases Involved in Lymphatic Vessel Remodeling

2017 ◽  
Vol 15 (1) ◽  
pp. 30-43 ◽  
Author(s):  
T. Jessica Gambino ◽  
Steven P. Williams ◽  
Carol Caesar ◽  
Daniel Resnick ◽  
Cameron J. Nowell ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Lymphatic Vessel ◽  
Three Dimensional ◽  
Lymphatic Endothelial Cell ◽  
Tube Formation ◽  
Vessel Remodeling ◽  
Tube Formation Assay

scholarly journals Cryptotanshinone Inhibits Lymphatic Endothelial Cell Tube Formation by Suppressing VEGFR-3/ERK and Small GTPase Pathways

2011 ◽  
Vol 4 (12) ◽  
pp. 2083-2091 ◽  
Author(s):  
Yan Luo ◽  
Wenxing Chen ◽  
Hongyu Zhou ◽  
Lei Liu ◽  
Tao Shen ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Lymphatic Endothelial Cell ◽  
Tube Formation ◽  
Small Gtpase

scholarly journals Macrophages define dermal lymphatic vessel calibre during development by regulating lymphatic endothelial cell proliferation

Development ◽  
2010 ◽  
Vol 137 (22) ◽  
pp. 3899-3910 ◽  
Author(s):  
E. J. Gordon ◽  
S. Rao ◽  
J. W. Pollard ◽  
S. L. Nutt ◽  
R. A. Lang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cell ◽  
Lymphatic Vessel ◽  
Lymphatic Endothelial Cell ◽  
Endothelial Cell Proliferation

scholarly journals The inhibitory effect of HKa in endothelial cell tube formation is mediated by disrupting the uPA-uPAR complex and inhibiting its signaling and internalization

2008 ◽  
Vol 295 (1) ◽  
pp. C257-C267 ◽  
Author(s):  
Yuchuan Liu ◽  
Dian J. Cao ◽  
Irma M. Sainz ◽  
Yan-Lin Guo ◽  
Robert W. Colman
Keyword(s):  
Endothelial Cell ◽  
Three Dimensional ◽  
Inhibitory Effect ◽  
Tube Formation ◽  
Physiological Concentration ◽  
Erk Activation ◽  
Erk Phosphorylation ◽  
And Migration ◽  
Induced Apoptosis

In two-dimensional (2-D) culture systems, we have previously shown that cleaved two-chain high-molecular-weight kininogen (HKa) or its domain 5 induced apoptosis by disrupting urokinase plasminogen activator (uPA) receptor (uPAR)-integrin signal complex formation. In the present study, we used a three-dimensional (3-D) collagen-fibrinogen culture system to monitor the effects of HKa on tube formation. In a 3-D system, HKa significantly inhibited tube and vacuole formation as low as 10 nM, which represents 1.5% of the physiological concentration of high-molecular-weigh kininogen (660 nM), without apparent apoptosis. However, HKa (300 nM) completely inhibited tube formation and increased apoptotic cells about 2-fold by 20–24 h of incubation. uPA-dependent ERK activation and uPAR internalization regulate cell survival and migration. In a 2-D system, we found that exogenous uPA-induced ERK phosphorylation and uPAR internalization were blocked by HKa. In a 3-D system, we found that not only uPA-uPAR association but also the activation of ERK were inhibited by HKa. HKa disrupts the uPA-uPAR complex, inhibiting the signaling pathways, and also inhibits uPAR internalization and regeneration to the cell surface, thereby interfering with uPAR-mediated cell migration, proliferation, and survival. Thus, our data suggest that the suppression of ERK activation and uPAR internalization by HKa contributes to the inhibition of tube formation. We conclude that in this 3-D collagen-fibrinogen gel, HKa modulates the multiple functions of uPAR in endothelial cell tube formation, a process that is closely related to in vivo angiogenesis.

scholarly journals Novel Mechanisms of Compromised Lymphatic Endothelial Cell Homeostasis in Obesity: The Role of Leptin in Lymphatic Endothelial Cell Tube Formation and Proliferation

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0158408 ◽  
Author(s):  
Akinori Sato ◽  
Ryuta Kamekura ◽  
Koji Kawata ◽  
Masaya Kawada ◽  
Sumito Jitsukawa ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Lymphatic Endothelial Cell ◽  
Tube Formation ◽  
Cell Homeostasis
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