scholarly journals Seroepidemiology ofTrypanosoma cruzi, Etiologic Agent of Chagas' Disease, in US Blood Donors

1997 ◽  
Vol 176 (4) ◽  
pp. 1047-1052 ◽  
Author(s):  
David A. Leiby ◽  
Elizabeth J. Read ◽  
Bruce A. Lenes ◽  
A. Jeffrey Yund ◽  
Robert J. Stumpf ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Blood Donors ◽  
Etiologic Agent

Major Kinds of Drug Targets in Chagas Disease or American Trypanosomiasis

2019 ◽  
Vol 20 (11) ◽  
pp. 1203-1216 ◽  
Author(s):  
Vilma G. Duschak
Keyword(s):  
Chagas Disease ◽  
Latin American ◽  
Drug Targets ◽  
Defense Mechanisms ◽  
Cysteine Proteinase ◽  
Parasitic Infection ◽  
Etiologic Agent ◽  
World Health ◽  
American Trypanosomiasis ◽  
Group I

American Trypanosomiasis, a parasitic infection commonly named Chagas disease, affects millions of people all over Latin American countries. Presently, the World Health Organization (WHO) predicts that the number of international infected individuals extends to 7 to 8 million, assuming that more than 10,000 deaths occur annually. The transmission of the etiologic agent, Trypanosoma cruzi, through people migrating to non-endemic world nations makes it an emergent disease. The best promising targets for trypanocidal drugs may be classified into three main groups: Group I includes the main molecular targets that are considered among specific enzymes involved in the essential processes for parasite survival, principally Cruzipain, the major antigenic parasite cysteine proteinase. Group II involves biological pathways and their key specific enzymes, such as Sterol biosynthesis pathway, among others, specific antioxidant defense mechanisms, and bioenergetics ones. Group III includes the atypical organelles /structures present in the parasite relevant clinical forms, which are absent or considerably different from those present in mammals and biological processes related to them. These can be considered potential targets to develop drugs with extra effectiveness and fewer secondary effects than the currently used therapeutics. An improved distinction between the host and the parasite targets will help fight against this neglected disease.

scholarly journals Treatment of T. cruzi infected human platelet concentrates with aminomethyltrimethyl psoralen (AMT) and ultravioleta (UV-A) light: preliminary results

1996 ◽  
Vol 29 (1) ◽  
pp. 47-49 ◽  
Author(s):  
Hélio Moraes-Souza ◽  
José Orlando Bordin ◽  
Leslie Bardossy ◽  
Morris A. Blajchman
Keyword(s):  
Ascorbic Acid ◽  
Chagas Disease ◽  
Untreated Control ◽  
Blood Donors ◽  
Human Platelet ◽  
Gentian Violet ◽  
Platelet Concentrates ◽  
Preliminary Results ◽  
Combined Use

The present measures adopted to prevent transfusion-associated Chagas' disease include screening of blood donors. and/or the inactivation of T. cruzi in collected blood using gentian violet (GV) as a trypanocidal agent. In this study, we investigated the efficacy of the combined use of AMT and UV-A in inactirating T. cruzi in infected human platelet cuncentrates. Human platelet concentrates were infected with T. cruzi (2x10/ml) of the Y strain transfered to PL 269 (Fenwal Laboratories) containers and treated with GV (250řg,/ml). and ascorbic acid (1 mg/ml); GV. ascorbic acid and UV-A; GV and UV-A; AMT (40/tG/ml) and ascorbic acid; AMT, ascorbic acid and UV-A; AMT and UV-A; UV-A alone; and untreated (control). All UV-A treated platelet concentrates were exposed to UV-A doses of 24, 92, 184, 276, 368 and 644 kj/m². and the microscopical research of active T. cruzi was performed, using the microhematocrit technique, 1, 6 and 24 hours after each treatment. A high number of active forms of T. cruzi was observed in all condictions, except when GV was used as the trypanocidal agent, providing evidence of the failure of AMT and UV-A in inactivating T cruzi in infected human platelet concentrates.

scholarly journals SEROPREVALENCE OF CHAGASIC INFECTION IN YOUNG INDIVIDUALS IN A BLOOD CENTER IN THE STATE OF SAO PAULO, BRAZIL

2013 ◽  
Vol 55 (4) ◽  
pp. 245-250 ◽  
Author(s):  
Elaine Cristina Navarro ◽  
Renata Leme Goto ◽  
Isabella Silva Ricoboni ◽  
Jose Eduardo Corrente ◽  
Rita Maria Saccomano Henriques ◽  
...  
Keyword(s):  
Public Health ◽  
Chagas Disease ◽  
Blood Donors ◽  
Public Health Problem ◽  
Sao Paulo ◽  
São Paulo ◽  
Blood Center ◽  
Negative Results ◽  
Central Regions ◽  
The City

SUMMARY This study aimed at estimating the number of cases of non-negative serological reactions to Chagas disease in blood donors at the Blood Center of Botucatu, São Paulo, Brazil, from 2003 to 2010 and at relating them to their cities of origin. Five hundred and seventy-four non-negative results for Chagas disease were evaluated. Of these, 371 (64.8%) were reagent, and 203 (35.4%) were inconclusive. The prevalence of Chagas disease in blood donors was 0.05%. There were, on average, 72 cases/year, and a prevalence of males was observed (64.8%). Forty-three (7.49%) individuals were 18 to 30 years old; 92 (16.02%) were 31 to 40; 147 (25.61%) 41 to 50, and 292 (50.87%) were older than 50 years. It was observed that 29.3% of females with reagent serology were at their fertile age (18 and 45 years). The majority of donors were originally from cities in the southwestern and central regions of São Paulo, but individuals from other states contributed with 20%. The provenance of most donors was the city of Botucatu/SP, followed by the city of Taquarituba/SP. Therefore, the profile of donors at this blood center favors the occurrence of a larger number of non-negative serological reactions. Although there has been a significant reduction in the number of new cases/year for this disease, it is still a public-health problem, and results suggest the need for new epidemiological assessments in the studied region.

BLOOD DONORS AND BLOOD COLLECTION: Testing blood donors for Chagas disease in the Paris area, France: first results after 18 months of screening

Transfusion ◽  
2009 ◽  
Vol 50 (3) ◽  
pp. 575-583 ◽  
Author(s):  
Marie-Hélène El Ghouzzi ◽  
Elisabeth Boiret ◽  
Françoise Wind ◽  
Claudine Brochard ◽  
Sébastien Fittere ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Blood Donors ◽  
Blood Collection ◽  
Paris Area ◽  
First Results

scholarly journals Screening of at‐risk blood donors for Chagas disease in non‐endemic countries: Lessons from a 2‐year experience in Tuscany, Italy

2020 ◽  
Author(s):  
Valentina D. Mangano ◽  
Marco Prato ◽  
Antonella Marvelli ◽  
Giovanna Moscato ◽  
Fabrizio Bruschi
Keyword(s):  
At Risk ◽  
Chagas Disease ◽  
Blood Donors

scholarly journals Prevalence of Chagas disease in blood donors at the Uberaba Regional Blood Center, Brazil, from 1995 to 2009

2012 ◽  
Vol 45 (6) ◽  
pp. 723-726 ◽  
Author(s):  
Laryssa Manso de Lima ◽  
Nathália Passos Alves ◽  
Valdirene de Fátima Barbosa ◽  
Gustavo Alves Pimenta ◽  
Helio Moraes-Souza ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Blood Donors ◽  
Retrospective Studies ◽  
Chi Square ◽  
Significant Drop ◽  
Blood Center ◽  
Epidemiologic Profile ◽  
Chi Square Test ◽  
Consistent Increase

INTRODUCTION: A retrospective study was conducted to assess the occurrence of blood donations that were ineligible due to Chagas disease infection from 1995 to 2009 at the Uberaba Regional Blood Center (HRU), Brazil, verify the tendency of this ineligibility, and describe the epidemiologic profile of the donors. METHODS: Retrospective studies of serological ineligibility due to Chagas disease, statistical analysis by means of the chi-square test and odds ratio, study of the tendencies using a dispersion graph and the linear correlation coefficient (r) were performed. RESULTS: In the period under study, a 0.2% serum prevalence of ineligibility due to Chagas disease was found, with a significant drop in ineligible donations from 2001 to 2009. Among the serum positive-donors, there was a significant predominance among those aged 30 years or above and non-single individuals. CONCLUSIONS: The results show a rate of occurrence that is lower than that described in literature, as well as a progressive drop during the 15 years under assessment. Such results are a consequence of systematic combat of the vector since the 70s and the progressive and consistent increase of returning donors, resulting in a drop of the contamination risk factor by means of blood transfusion and in the improvement of the quality of hemotherapy practices in the HRU.

scholarly journals High-Throughput Identification of the Rhodnius prolixus Midgut Proteome Unravels a Sophisticated Hematophagic Machinery

Proteomes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 16 ◽  
Author(s):  
Radouane Ouali ◽  
Karen Caroline Valentim de Brito ◽  
Didier Salmon ◽  
Sabrina Bousbata
Keyword(s):  
Digestive Tract ◽  
Chagas Disease ◽  
High Throughput ◽  
Control Strategies ◽  
Anterior Part ◽  
Rhodnius Prolixus ◽  
Etiologic Agent ◽  
Parasitic Infections ◽  
Developmental Cycle ◽  
Starting Point

Chagas disease is one of the most common parasitic infections in Latin America, which is transmitted by hematophagous triatomine bugs, of which Rhodnius prolixus is the vector prototype for the study of this disease. The protozoan parasite Trypanosoma cruzi, the etiologic agent of this disease, is transmitted by the vector to humans through the bite wound or mucosa. The passage of the parasite through the digestive tract of its vector constitutes a key step in its developmental cycle. Herewith, by a using high-throughput proteomic tool in order to characterize the midgut proteome of R. prolixus, we describe a set of functional groups of proteins, as well as the biological processes in which they are involved. This is the first proteomic analysis showing an elaborated hematophagy machinery involved in the digestion of blood, among which, several families of proteases have been characterized. The evaluation of the activity of cathepsin D proteases in the anterior part of the digestive tract of the insect suggested the existence of a proteolytic activity within this compartment, suggesting that digestion occurs early in this compartment. Moreover, several heat shock proteins, blood clotting inhibitors, and a powerful antioxidant enzyme machinery against reactive oxygen species (ROS) and cell detoxification have been identified. Highlighting the complexity and importance of the digestive physiology of insects could be a starting point for the selection of new targets for innovative control strategies of Chagas disease.

scholarly journals Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results

2020 ◽  
Author(s):  
Márcia M Ferreira-Silva ◽  
Gilberto de Araújo Pereira ◽  
Virmondes Rodrigues-Júnior ◽  
Wendell SF Meira ◽  
Fernando V Basques ◽  
...  
Keyword(s):  
Performance Analysis ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Blood Donors ◽  
Immunodiagnostic Tests
Sign in / Sign up

Export Citation Format

Share Document