scholarly journals Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results

2020 ◽  
Author(s):  
Márcia M Ferreira-Silva ◽  
Gilberto de Araújo Pereira ◽  
Virmondes Rodrigues-Júnior ◽  
Wendell SF Meira ◽  
Fernando V Basques ◽  
...  
Keyword(s):  
Performance Analysis ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Blood Donors ◽  
Immunodiagnostic Tests

scholarly journals Disagreement between PCR and serological diagnosis of Trypanosoma cruzi infection in blood donors from a Colombian endemic region

Biomédica ◽  
2021 ◽  
Vol 41 (Supl. 1) ◽  
pp. 47-59
Author(s):  
Liliana Torcoroma García Sánchez ◽  
Jhancy Rocío Aguilar Jiménez ◽  
Marly Yojhana Bueno ◽  
Erika Marcela Moreno Moreno ◽  
Herminia Ramírez ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Blood Donors ◽  
False Negative ◽  
Chronic Phase ◽  
Antibody Detection ◽  
Low Grade ◽  
Endemic Region ◽  
Infected People ◽  
Cruzi Infection

Introduction: Chagas’ disease is the leading cause of infectious myocarditis worldwide. This infection caused by Trypanosoma cruzi is usually life-long and asymptomatic; however, the third part of infected people can develop severe or even fatal cardiomyopathy. As the parasitemia in the chronic phase is both low-grade and intermittent, T. cruzi infection is principally detected by serology, although this method has sensitivity and specificity limitations.Objective: To determine the level of agreement between serologic and molecular tests in 658 voluntary blood donors from six provinces in the Colombian department of Santander.Materials and methods: We evaluated an array of diagnostic technologies by cross-section sampling performing a serological double diagnostic test for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott), and DNA detection by polymerase chain reaction (PCR). We collected the demographic, clinical, and epidemiological information of participants. The sample size was calculated using Epidat™ and the statistical analysis was done with Stata 12.1™.Results: PCR was six times more sensitive in detecting T. cruzi infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 - 0.59). In contrast, serology showed a sensitivity of 16.7% (95%CI: 2.09 - 48.4) and a specificity of 100% (95%CI: 99.4 - 100). All seropositive samples were found to be positive by PCR.Conclusions: The implementation of PCR as a complementary method for screening donors could reduce the probability of false negative and the consequent risk of transfusional-transmission of Chagas’ disease, especially in endemic regions.

scholarly journals Benznidazole therapy for Chagas disease in asymptomatic Trypanosoma cruzi -seropositive former blood donors: evaluation of the efficacy of different treatment regimens

2016 ◽  
Vol 49 (6) ◽  
pp. 713-720 ◽  
Author(s):  
André Pires Antunes ◽  
Antônio Luiz Pinho Ribeiro ◽  
Ester Cerdeira Sabino ◽  
Marise Fagundes Silveira ◽  
Cláudia Di Lorenzo Oliveira ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Blood Donors ◽  
Treatment Regimens

scholarly journals Prevalence of Trypanosoma Cruzi antibodies in blood donors from the Sao Paulo State, Brazil, between 2012 and 2014

2017 ◽  
Vol 11 (03) ◽  
pp. 277-281 ◽  
Author(s):  
Svetoslav Nanev Slavov ◽  
Katia Kaori Otaguiri ◽  
Mariana Tomazini Pinto ◽  
Vanderléia Bárbaro Valente ◽  
Eugênia Maria Amorim Ubiali ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Blood Donors ◽  
Significant Risk ◽  
Protozoan Parasite ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State ◽  
The Cost

Introduction: American tripanosomiasis (Chagas disease), the second most neglected disease in the world, is caused by the protozoan parasite Trypanosoma cruzi. Though natural transmission by insect vectors has been controlled, there is significant risk of T. cruzi transmission by blood transfusion in non-endemic regions, generally due to immigration processes from endemic areas. Methodology: The objective of this study was to evaluate anti-T. cruzi seroprevalence in blood donors from the western part of São Paulo State, Brazil, by serologic and immunofluorescence confirmation tests for the period between 2012 and 2014. Currently, this region is regarded as a non-endemic area for Chagas disease. Results: The confirmed overall T. cruzi seroprevalence among blood donors was 0.10%, which can be considered low compared to other Brazilian regions. Nevertheless, the distribution of the anti-T. cruzi antibodies within the examined region was uneven, and some areas of significantly higher prevalence were observed. Conclusions: We could consider two tendencies in the prevalence of T. cruzi: (i) residual older undiagnosed cases from São Paulo State, and (ii) immigration from endemic Brazilian or South American regions. The discordance obtained for T. cruzi prevalence by serologic and immunofluorescence methods demonstrates that more specific routine diagnosis is needed to diminish the cost of the assays and the loss of blood supply once all seropositive blood bags are immediately discarded.

scholarly journals EVALUATION OF CROSS REACTIVITY BETWEEN Trypanosoma cruzi AND Leishmania infantum IN SEROLOGICALLY INELIGIBLE BLOOD DONORS DUE TO CHAGAS DISEASE

2017 ◽  
Vol 46 (1) ◽  
pp. 113 ◽  
Author(s):  
Márcia Maria Ferreira-Silva ◽  
Silvio Fernando Guimarães Carvalho ◽  
Jamille Fernandes Lula ◽  
Leandro De Freitas Teles ◽  
Fernando Valadares Basques ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Leishmania Infantum ◽  
Blood Donors ◽  
Cross Reactivity

Trypanosoma cruzi screening in Texas blood donors, 2008–2012

2014 ◽  
Vol 144 (5) ◽  
pp. 1010-1013 ◽  
Author(s):  
M. N. GARCIA ◽  
L. WOC-COLBURN ◽  
S. N. ROSSMANN ◽  
R. L. TOWNSEND ◽  
S. L. STRAMER ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Older Persons ◽  
Disease Burden ◽  
Blood Donors ◽  
Blood Donation ◽  
Economic Losses ◽  
Minority Populations ◽  
Significant Disease ◽  
Zip Code

SUMMARYChagas disease is an important emerging disease in Texas that results in cardiomyopathy in about 30% of those infected with the parasite Trypanosoma cruzi. Between the years 2008 and 2012, about 1/6500 blood donors were T. cruzi antibody-confirmed positive. We found older persons and minority populations, particularly Hispanic, at highest risk for screening positive for T. cruzi antibodies during routine blood donation. Zip code analysis determined that T. cruzi is associated with poverty. Chagas disease has a significant disease burden and is a cause of substantial economic losses in Texas.

Dynamics of T Cells Repertoire During Trypanosoma cruzi Infection and its Post-Treatment Modulation

2019 ◽  
Vol 26 (36) ◽  
pp. 6519-6543 ◽  
Author(s):  
Adriana Egui ◽  
Paola Lasso ◽  
Elena Pérez-Antón ◽  
M. Carmen Thomas ◽  
Manuel Carlos López
Keyword(s):  
Immune Response ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Cardiac Tissue ◽  
Acute Infection ◽  
Clinical Status ◽  
Chronic Phase ◽  
Parasite Load ◽  
Chronic Patients ◽  
Cruzi Infection

Chagas disease courses with different clinical phases and has a variable clinical presentation and progression. The acute infection phase mostly exhibits a non-specific symptomatology. In the absence of treatment, the acute phase is followed by a chronic phase, which is initially asymptomatic. This chronic asymptomatic phase of the disease is characterized by a fragile balance between the host’s immune response and the parasite replication. The loss of this balance is crucial for the progression of the sickness. The virulence and tropism of the T. cruzi infecting strain together to the inflammation processes in the cardiac tissue are the main factors for the establishment and severity of the cardiomyopathy. The efficacy of treatment in chronic Chagas disease patients is controversial. However, several studies carried out in chronic patients demonstrated that antiparasitic treatment reduces parasite load in the bloodstream and leads to an improvement in the immune response against the Trypanosoma cruzi parasite. The present review is mainly focused on the cellular patterns associated to the clinical status and the evolution of the disease in chronic patients, as well as the effectiveness of the treatment related to T. cruzi infection control. Therefore, an emphasis is placed on the dynamics of specific-antigens T cell subpopulations, their memory and activation phenotypes, their functionality and their contribution to pathogenesis or disease control, as well as their association with risk of congenital transmission of the parasite.

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