scholarly journals Antigenic and Genetic Diversity among the Attachment Proteins of Group A Respiratory Syncytial Viruses That Have Caused Repeat Infections in Children

1998 ◽  
Vol 178 (4) ◽  
pp. 925-932 ◽  
Author(s):  
Wayne M. Sullender ◽  
Maurice A. Mufson ◽  
Gregory A. Prince ◽  
Larry J. Anderson ◽  
Gail W. Wertz
Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 674
Author(s):  
Anton Yuzhakov ◽  
Ksenia Yuzhakova ◽  
Nadezhda Kulikova ◽  
Lidia Kisteneva ◽  
Stanislav Cherepushkin ◽  
...  

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.


1991 ◽  
Vol 65 (10) ◽  
pp. 5425-5434 ◽  
Author(s):  
W M Sullender ◽  
M A Mufson ◽  
L J Anderson ◽  
G W Wertz

2003 ◽  
Vol 71 (2) ◽  
pp. 305-312 ◽  
Author(s):  
Sandra Frabasile ◽  
Adriana Delfraro ◽  
Luj�n Facal ◽  
Cristina Videla ◽  
M�nica Galiano ◽  
...  

1991 ◽  
Vol 5 (12) ◽  
pp. 2947-2952 ◽  
Author(s):  
K. F. Jones ◽  
O. Schneewind ◽  
J. M. Koomey ◽  
V. A. Fischetti

2011 ◽  
Vol 75 (3) ◽  
pp. 245-251 ◽  
Author(s):  
Elżbieta Cieślak ◽  
Wojciech Paul ◽  
Michał Ronikier

An extremely endangered population of <em>Viola uliginosa</em> Besser at the classical locality of this taxon has been studied. The AFLP analysis was based on 18 specimens of <em>V. uliginosa</em> (about 10% of preserved individuals); additionally, two individuals of <em>V. riviniana</em> were included in the data set as the out group. A high genetical uniformity of the whole population (similarity indexes close to 1) was detected. It was not correlated significantly with the spatial distribution of the plants. The study serves as a basis for practical conservation measures and at the same time as a starting point for a more extensive research on the genetical variability of the species throughout its range.


Author(s):  
JULIA WANGUI ◽  
David Nokes ◽  
Victor Mobegi ◽  
James Otieno ◽  
Charles Agoti ◽  
...  

Background: Human respiratory syncytial virus (HRSV) is a major cause of severe viral acute respiratory illness and contributes significantly to severe pneumonia cases in Africa. Little is known about its spatial-temporal distribution as defined by its genetic diversity. Methods: A retrospective study conducted utilizing archived nasopharyngeal specimens from patients attending outpatient clinics in hospitals located in five demographically and climatically distinct regions of Kenya; Coast, Western, Highlands, Eastern and Nairobi. The viral total RNA was extracted and tested using multiplex real time RT-PCR (reverse transcriptase polymerase chain reaction). A segment of the G-gene was amplified using one-step RT-PCR and sequenced by Sanger di-deoxy method. Bayesian analysis of phylogeny was utilized and subsequently median joining methods for haplotype network reconstruction. Results: Three genotypes of HRSVA were detected; GA5 (14.0%), GA2 (33.1%) and NA1 (52.9%). HRSVA prevalence varied by location from 33% to 13.2% in the Highlands and the Eastern regions respectively. The mean nucleotide diversity (Pi(π)) varied by genotype: highest of 0.018 for GA5 and lowest of 0.005 for NA1. A total of 58 haplotypes were identified (GA5 10; GA2 20; NA1 28). These haplotypes were introduced into the population locally by single haplotypes and additional subsidiary seeds amongst the GA2 and the NA1 haplotypes. Conclusions: HRSVA was found across all the regions throughout the study period and comprised three genotypes; GA5, GA2 and NA1 genotypes. The genotypes were disproportionately distributed across the regions with GA5 gradually increasing towards the Western zones and decreasing towards the Eastern zones of the country.


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