scholarly journals Improved Diagnosis of the Etiology of Community-Acquired Pneumonia with Real-Time Polymerase Chain Reaction

2005 ◽  
Vol 41 (3) ◽  
pp. 345-351 ◽  
Author(s):  
K. E. Templeton ◽  
S. A. Scheltinga ◽  
W. C. J. F. M. van den Eeden ◽  
W. A. Graffelman ◽  
P. J. van den Broek ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Community Acquired Pneumonia ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Viruses by Multiplex Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Nonresponding Community-Acquired Pneumonia

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Hao Zhang ◽  
Yinling Han ◽  
Zhangchu Jin ◽  
Yinghua Ying ◽  
Fen Lan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bronchoalveolar Lavage ◽  
Viral Infection ◽  
Real Time ◽  
Viral Infections ◽  
Ground Glass Opacity ◽  
Community Acquired Pneumonia ◽  
Ground Glass ◽  
Chain Reaction ◽  
Polymerase Chain

Background. Nonresponding pneumonia is responsible for the most mortality of community-acquired pneumonia (CAP). However, thus far, it is not clear whether viral infection plays an important role in the etiology of nonresponding CAP and whether there is a significant difference in the clinical characteristics between viral and nonviral nonresponding CAP. Methods. From 2016 to 2019, nonresponding CAP patients were retrospectively enrolled in our study. All patients received bronchoalveolar lavage (BAL) and virus detection in BAL fluid by multiplex real-time polymerase chain reaction (PCR), and clinical, laboratory, and radiographic data were collected. Results. A total of 43 patients were included. The median age was 62 years, and 65.1% of patients were male. Overall, 20 patients (46.5%) were identified with viral infection. Of these viruses, influenza virus (n = 8) and adenovirus (n = 7) were more frequently detected, and others included herpes simplex virus, human enterovirus, cytomegalovirus, human coronavirus 229E, rhinovirus, and parainfluenza virus. Compared with nonviral nonresponding CAP, only ground-glass opacity combined with consolidation was a more common imaging manifestation in viral nonresponding CAP. However, no obvious differences were found in clinical and laboratory findings between the presence and the absence of viral infections. Conclusions. Viral infections were particularly frequent in adults with nonresponding CAP. The ground-glass opacity combined with consolidation was a specific imaging manifestation for viral nonresponding CAP, while the clinical and laboratory data showed no obvious differences between viral and nonviral nonresponding CAP.

Detection of respiratory viruses and Legionella spp. by real-time polymerase chain reaction in patients with community acquired pneumonia

2009 ◽  
Vol 41 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Bram M.W. Diederen ◽  
Menno M. Van Der Eerden ◽  
Fer Vlaspolder ◽  
Wim G. Boersma ◽  
Jan A.J.W. Kluytmans ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Respiratory Viruses ◽  
Community Acquired Pneumonia ◽  
Chain Reaction ◽  
Polymerase Chain

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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