scholarly journals Detection of Viruses by Multiplex Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Nonresponding Community-Acquired Pneumonia

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Hao Zhang ◽  
Yinling Han ◽  
Zhangchu Jin ◽  
Yinghua Ying ◽  
Fen Lan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bronchoalveolar Lavage ◽  
Viral Infection ◽  
Real Time ◽  
Viral Infections ◽  
Ground Glass Opacity ◽  
Community Acquired Pneumonia ◽  
Ground Glass ◽  
Chain Reaction ◽  
Polymerase Chain

Background. Nonresponding pneumonia is responsible for the most mortality of community-acquired pneumonia (CAP). However, thus far, it is not clear whether viral infection plays an important role in the etiology of nonresponding CAP and whether there is a significant difference in the clinical characteristics between viral and nonviral nonresponding CAP. Methods. From 2016 to 2019, nonresponding CAP patients were retrospectively enrolled in our study. All patients received bronchoalveolar lavage (BAL) and virus detection in BAL fluid by multiplex real-time polymerase chain reaction (PCR), and clinical, laboratory, and radiographic data were collected. Results. A total of 43 patients were included. The median age was 62 years, and 65.1% of patients were male. Overall, 20 patients (46.5%) were identified with viral infection. Of these viruses, influenza virus (n = 8) and adenovirus (n = 7) were more frequently detected, and others included herpes simplex virus, human enterovirus, cytomegalovirus, human coronavirus 229E, rhinovirus, and parainfluenza virus. Compared with nonviral nonresponding CAP, only ground-glass opacity combined with consolidation was a more common imaging manifestation in viral nonresponding CAP. However, no obvious differences were found in clinical and laboratory findings between the presence and the absence of viral infections. Conclusions. Viral infections were particularly frequent in adults with nonresponding CAP. The ground-glass opacity combined with consolidation was a specific imaging manifestation for viral nonresponding CAP, while the clinical and laboratory data showed no obvious differences between viral and nonviral nonresponding CAP.

scholarly journals Seorang Anak Perempuan Probable Covid-19 dengan Keterlibatan Ginjal (Laporan kasus)

2020 ◽  
Vol 7 (1A) ◽  
pp. 207-213
Author(s):  
Omega Mellyana ◽  
Nur Latifah ◽  
Marcella Trixie ◽  
Frederika Mardiana ◽  
Moh Syarofil Anam ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bacterial Infections ◽  
Ground Glass Opacity ◽  
Ground Glass ◽  
Post Mortem ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Chest X Ray ◽  
Polymerase Chain

Latar belakang: Kasus Probable Covid-19 adalah kasus penderita  dengan gambaran klinis dan pemeriksaan penunjang yang meyakinkan Covid-19 namun tidak terkonfirmasi dengan  pemeriksaan real-time polymerase chain reaction (RT-PCR). Kasus probable  banyak menimbulkan  kekhawatiran karena risiko penularan dan keraguan  dalam tata laksana baik bagi dokter, perawat, dan  penanggung jawab  pasien. Tujuan penulisan artikel ini adalah untuk melaporkan kasus seorang anak dengan probable Covid-19  dan mendiskusikan  kemungkinan diagnosis banding lain sebagai pemikiran di tengah pandemi Covid-19 guna pengelolaan pasien yang lebih optimal. Kasus:  Anak perempuan 14 tahun 5 bulan dengan keluhan utama batuk selama dua minggu sebelum masuk rumah sakit,  disertai demam, diare dan sesak. Selama perawatan, sesak makin bertambah. Tiga hari perawatan ditemukan oliguria, proteinuria, anemia, leukositosis, trombositopenia, hipersegmentasi neutrofil, limfopenia, peningkatan prokalsitonin, hipoalbuminemia dan penurunan fungsi ginjal (51 ml/menit/1,73 m2). Gambaran rontgen dada menunjukkan bronkopneumonia dan kardiomegali. MSCT dada terdapat gambaran konsolidasi dan ground glass appearance (GGO) di kedua paru mendukung ke arah Covid-19. Pada hari perawatan ke 16 anak mengalami gagal nafas, hemoptoe, penurunan kesadaran  hingga meninggal.   Swab RT-PCR 3 kali negatif (selama perawatan dan post mortem). Ringkasan : Infeksi Covid-19 adalah penyebab infeksi saluran nafas yang serius dan berat. Telah dilaporkan seorang anak perempuan 14 tahun 5 bulan yang meninggal karena Probable Covid-19. Di tengah pandemi Covid-19 ini seorang dokter  perlu meningkatkan kewaspadaan yang tinggi terhadap infeksi virus atau bakteri lain untuk memperbaiki tata laksana dan luaran  pada penderita. Kata kunci: Covid-19, probable, ground glass opacity, RT-PCR   Background: Probable Covid-19 cases are patients with clinical features and convincing investigations for covid-19 but there is  not confirmed by real time polymerase chain reaction (RT-PCR). Probable cases raise many concerns because of the risk of transmission and doubt in good management for the doctor / nurse in charge of the patient. The purpose of writing was to report the child with probable Covid-19 and provide a discussion of possible other differential diagnoses as thoughts in the midst of the Covid-19 pandemic for optimal management of sufferers. Case: A girl 14 years 5 months old had a cough for two weeks before admission, accompanied by fever, diarrhea and breathlessness. During treatment, the shortness of breath increased, three days of treatment found oliguria, proteinuria, hematuria, anemia, leukocytosis, thrombocytopenia, neutrophil hypersegmentation, lymphopenia, increased procalcitonin, hypoalbuminemia and decreased renal function (51 ml / min / 1.73 m2). Chest x-ray of bronchopneumonia and cardiomegaly. In the finding of  chest computed tomography scan showed consolidation and ground glass appearance (GGO) in both lungs supporting the diagnose  of Covid-19. On the 16th day of treatment, the child suffered respiratory failure, hemoptoes, decreased consciousness until death. RT-PCR swabs were 3 times negative (during treatment and post mortem). Summary: Covid-19 is a serious and severe cause of respiratory tract infection. It has been reported that a girl of 14 years and 5 months who died of Probable Covid-19 has been reported. During the Covid-19 pandemic, a doctor  may need  to increase high awareness of other viral or bacterial infections to improve management  and outcome of patients in the future. Key word: Covid-19,  Children,  Ground Glass Opacity, RT_PCR

scholarly journals Chest CT target sign in a couple with COVID-19 pneumonia

2020 ◽  
Vol 53 (4) ◽  
pp. 252-254 ◽  
Author(s):  
C. Isabela Silva Müller ◽  
Nestor L. Müller
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Ground Glass Opacity ◽  
Chest Ct ◽  
Ground Glass ◽  
Chain Reaction ◽  
Target Sign ◽  
Peripheral Ring ◽  
Polymerase Chain

Abstract We describe a target sign on chest CT characterized by a combination of peripheral ring-like opacity and a central nodular ground-glass opacity surrounding a vessel in a couple with COVID-19 pneumonia confirmed by real-time reverse transcriptase fluorescence polymerase chain reaction sputum analysis.

Sign in / Sign up

Export Citation Format

Share Document