Detection of Streptococcus pneumoniae and Identification of Pneumococcal Serotypes by Real-Time Polymerase Chain Reaction Using Blood Samples from Italian Children ≤5 Years of Age with Community-Acquired Pneumonia

2011 ◽  
Vol 17 (3) ◽  
pp. 419-424 ◽  
Author(s):  
Anna Marchese ◽  
Susanna Esposito ◽  
Erika Coppo ◽  
Giovanni A. Rossi ◽  
Alberto Tozzi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Community Acquired Pneumonia ◽  
Pneumococcal Serotypes ◽  
Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain ◽  
Italian Children

scholarly journals Detection of Viruses by Multiplex Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Nonresponding Community-Acquired Pneumonia

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Hao Zhang ◽  
Yinling Han ◽  
Zhangchu Jin ◽  
Yinghua Ying ◽  
Fen Lan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bronchoalveolar Lavage ◽  
Viral Infection ◽  
Real Time ◽  
Viral Infections ◽  
Ground Glass Opacity ◽  
Community Acquired Pneumonia ◽  
Ground Glass ◽  
Chain Reaction ◽  
Polymerase Chain

Background. Nonresponding pneumonia is responsible for the most mortality of community-acquired pneumonia (CAP). However, thus far, it is not clear whether viral infection plays an important role in the etiology of nonresponding CAP and whether there is a significant difference in the clinical characteristics between viral and nonviral nonresponding CAP. Methods. From 2016 to 2019, nonresponding CAP patients were retrospectively enrolled in our study. All patients received bronchoalveolar lavage (BAL) and virus detection in BAL fluid by multiplex real-time polymerase chain reaction (PCR), and clinical, laboratory, and radiographic data were collected. Results. A total of 43 patients were included. The median age was 62 years, and 65.1% of patients were male. Overall, 20 patients (46.5%) were identified with viral infection. Of these viruses, influenza virus (n = 8) and adenovirus (n = 7) were more frequently detected, and others included herpes simplex virus, human enterovirus, cytomegalovirus, human coronavirus 229E, rhinovirus, and parainfluenza virus. Compared with nonviral nonresponding CAP, only ground-glass opacity combined with consolidation was a more common imaging manifestation in viral nonresponding CAP. However, no obvious differences were found in clinical and laboratory findings between the presence and the absence of viral infections. Conclusions. Viral infections were particularly frequent in adults with nonresponding CAP. The ground-glass opacity combined with consolidation was a specific imaging manifestation for viral nonresponding CAP, while the clinical and laboratory data showed no obvious differences between viral and nonviral nonresponding CAP.

scholarly journals Community‐Acquired Bacteremic Pneumococcal Pneumonia in Children: Diagnosis and Serotyping by Real‐Time Polymerase Chain Reaction Using Blood Samples

2010 ◽  
Vol 51 (9) ◽  
pp. 1042-1049 ◽  
Author(s):  
Massimo Resti ◽  
Maria Moriondo ◽  
Martina Cortimiglia ◽  
Giuseppe Indolfi ◽  
Clementina Canessa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Pneumococcal Pneumonia ◽  
Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain ◽  
Bacteremic Pneumococcal Pneumonia
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