scholarly journals False‐Positive Gonorrhea Test Results with a Nucleic Acid Amplification Test: The Impact of Low Prevalence on Positive Predictive Value

2004 ◽  
Vol 38 (6) ◽  
pp. 814-819 ◽  
Author(s):  
Alan R. Katz ◽  
Paul V. Effler ◽  
Roy G. Ohye ◽  
Barbara Brouillet ◽  
Maria Veneranda C. Lee ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Positive Predictive Value ◽  
Predictive Value ◽  
False Positive ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Test Results ◽  
Low Prevalence ◽  
The Impact

scholarly journals Low Positive Predictive Value of a Nucleic Acid Amplification Test for NongenitalNeisseria gonorrhoeaeInfection in Homosexual Men

2008 ◽  
Vol 47 (2) ◽  
pp. e25-e27 ◽  
Author(s):  
Leon P. McNally ◽  
David J. Templeton ◽  
Fengyi Jin ◽  
Andrew E. Grulich ◽  
Basil Donovan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Positive Predictive Value ◽  
Predictive Value ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Homosexual Men

scholarly journals Evaluation of the QiagenartusC. difficile QS-RGQ Kit for Detection of Clostridium difficile Toxins A and B in Clinical Stool Specimens

2015 ◽  
Vol 53 (6) ◽  
pp. 1942-1944 ◽  
Author(s):  
Nathalie Jazmati ◽  
Pia Wiegel ◽  
Božica Ličanin ◽  
Georg Plum
Keyword(s):  
Nucleic Acid ◽  
Clostridium Difficile ◽  
Positive Predictive Value ◽  
Negative Predictive Value ◽  
Predictive Value ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Content Type ◽  
Stool Specimens ◽  
Sensitivity Specificity

We compared the QiagenartusC. difficile QS-RGQ kit, a new nucleic acid amplification test for the detection ofClostridium difficiletoxins in stool specimens, with the Cepheid XpertC. difficiletest. The sensitivity, specificity, positive predictive value, and negative predictive value for the QiagenartusC. difficile QS-RGQ test were 100%, 89.5%, 60.9%, and 100%, and those for the Cepheid XpertC. difficiletest were 100%, 90%, 62.2%, and 100%, respectively.

Evaluation of anopagene-based nucleic acid amplification test for detection ofNeisseria gonorrhoeaein urogenital samples in North India

2012 ◽  
Vol 140 (11) ◽  
pp. 2110-2116 ◽  
Author(s):  
R. VERMA ◽  
S. SOOD ◽  
M. BALA ◽  
N. MAHAJAN ◽  
A. KAPIL ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Positive Predictive Value ◽  
Predictive Value ◽  
Nucleic Acid Amplification ◽  
North India ◽  
Rrna Gene ◽  
New Delhi ◽  
Sensitivity Specificity ◽  
Low Prevalence ◽  
Pcr Targeting

SUMMARYDue to the poor positive predictive value of nucleic acid amplification tests (NAATs) for gonorrhoea when applied to a low-prevalence setting, current guidelines recommend the use of supplementary polymerase chain reaction (PCR) targeting a different gene for confirmation of true positives in urogenital specimens. This study sought to standardize and evaluate performance of an in-houseopagene-based PCR assay for gonorrhoea compared to assays targeting theporApseudogene and 16S rRNA gene. Four hundred samples (300 endocervical, 100 urethral swabs) from patients attending STD clinics in New Delhi, India were used. The sensitivity, specificity, positive predictive value and negative predictive value of theopa-based PCR were 100%, 97·9%, 89·5% and 100%, respectively. In females, the use of NAATs provided enhanced diagnosis of gonorrhoea.

scholarly journals Prospective study to find out the role of gastric aspirate examination by Ziehl-Neelsen staining (ZN staining) and cartridge based nucleic acid amplification test (CB-NAAT) as a diagnostic method in childhood tuberculosis

2018 ◽  
Vol 5 (4) ◽  
pp. 1609
Author(s):  
Akansha Arora ◽  
Anil Jain ◽  
B. S. Karnawat ◽  
Rakesh Kumawat
Keyword(s):  
Nucleic Acid ◽  
Predictive Value ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Mantoux Test ◽  
P Value ◽  
Specific Method ◽  
Gastric Aspirate ◽  
Tuberculosis In Children

Background: Tuberculosis in children has been relatively neglected mainly because clinical diagnosis has low specificity, radiological interpretation is subject to inter-observer variability and the tuberculin skin test is a marker of exposure, not disease. The recent introduction of Cartridge based nucleic acid amplification test has significantly transformed the diagnostics of tuberculosis in adults but its application for Paediatric Tuberculosis is under evaluation. Therefore, authors conducted a study on role of gastric aspirate examination by ZN stain and Cartridge based nucleic acid amplification test in the diagnosis of childhood Tuberculosis.Methods: Authors did a prospective hospital-based study from Nov 2016 to Nov 2017 consisting of 100 randomly selected patients suspected of tuberculosis who had their gastric aspirate tested for CBNAAT and ZN stain for acid fast bacilli (AFB) along with Mantoux test and other routine investigations. Chi square test was used.Results: Culture positive tuberculosis was found in 21 out of 100 children. The sensitivity, specificity, positive predictive value and negative predictive value for CBNAAT were 76.1%, 98.7%, 94.1% and 93.9% and for ZN stain were 47.6%, 98.7%, 90.9% and 87.6% respectively. Positive history of contact (p value 0.0217), reactive Mantoux test (p value < 0.001) and low socioeconomic status were independently associated with a positive CBNAAT result.Conclusions: Analysis of gastric aspirate samples with CBNAAT is a sensitive and specific method for rapid diagnosis of pulmonary tuberculosis in children who cannot produce sputum. Compared with microscopy, CBNAAT offers better sensitivity and its scale up will improve access to tuberculosis diagnostics in children.

scholarly journals Supplementary Testing Is Not Required in the cobas 4800 CT/NG Test for Neisseria gonorrhoeae Weak-Positive Urogenital Samples

2014 ◽  
Vol 53 (1) ◽  
pp. 327-328 ◽  
Author(s):  
Collette Bromhead ◽  
Nadika Liyanarachchy ◽  
Julia Mayes ◽  
Arlo Upton ◽  
Michelle Balm
Keyword(s):  
Nucleic Acid ◽  
Neisseria Gonorrhoeae ◽  
Nucleic Acid Amplification Test ◽  
Pretest Probability ◽  
Nucleic Acid Amplification ◽  
Test Results ◽  
Content Type

Weak-positiveNeisseria gonorrhoeaenucleic acid amplification test results are difficult to interpret. We show that the frequency of unconfirmedN. gonorrhoeaeresults from the cobas 4800 test rises exponentially after 38.0 cycles, where the likelihood of an unconfirmed result exceeds 29%. Supplementary testing of such samples should be avoided; instead, treatment should be based on clinical pretest probability.

scholarly journals Diagnosis of pulmonary tuberculosis with cartridge based nucleic acid amplification test and light emitting diode fluorescent microscopy: a comparative study

2019 ◽  
Vol 6 (5) ◽  
pp. 1580 ◽  
Author(s):  
Pratik Kumar ◽  
Puneet Bhardwaj
Keyword(s):  
Nucleic Acid ◽  
Pulmonary Tuberculosis ◽  
Negative Predictive Value ◽  
Predictive Value ◽  
Light Emitting Diode ◽  
Fluorescent Microscopy ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Smear Microscopy ◽  
Light Emitting

Background: Due to low sensitivity and inability to detect drug resistance, smear microscopy limits its impact on TB control. Culture methods and drug susceptibility testing is complex, time consuming, and takes around 6-8 weeks. A new diagnostic test, cartridge based nucleic acid amplification test (CBNAAT) was developed based on real-time polymerase chain reaction (RT PCR). Objective of this study to compare the results of CBNAAT for diagnosis of pulmonary tuberculosis with LED fluorescent microscopy and sputum culture.Methods: A cross-sectional study was conducted in the department of Chest and TB, CIMS, Bilaspur. Each Sputum sample of presumptive TB patients were tested with CBNAAT, sputum smear  microscopy by light emitting diode (LED) fluorescent microscopy (FM) and solid and liquid culture for diagnosis of Tuberculosis. Results of CBNAAT, Fluorescent Microscopy and Culture for detection of Mycobacterium Tuberculosis were compared.Results: The sensitivity and specificity for CBNAAT were 97% and 100% respectively; while that for Fluorescent microscopy were 70% and 100% respectively. The positive and negative predictive value for CBNAAT was 100% and 96% respectively. The positive and negative predictive value for Fluorescent microscopy was 100% and 73% respectively.Conclusion: CBNAAT is having high sensitivity and specificity for diagnosis of pulmonary tuberculosis. It should be routinely used under national health programme to detect a tuberculosis case efficiently.

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