Chlamydia trachomatis RNA in the environment: is there potential for false-positive nucleic acid amplification test results?

2008 ◽  
Vol 84 (2) ◽  
pp. 107-110 ◽  
Author(s):  
E Meader ◽  
J Waters ◽  
M Sillis
Keyword(s):  
Nucleic Acid ◽  
Chlamydia Trachomatis ◽  
False Positive ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Test Results

Chlamydia trachomatis infection positivity rates determined by nucleic acid amplification test in patients of hospitals in the northeastern region of Ukraine

2017 ◽  
Vol 28 (14) ◽  
pp. 1405-1409
Author(s):  
Alexei Belozorov ◽  
Olga Fedets ◽  
Tatjana Chastii ◽  
Elena Milutina ◽  
Oksana Sokol ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Chlamydia Trachomatis ◽  
Chlamydial Infection ◽  
Age Groups ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Test Results ◽  
Chlamydia Trachomatis Infection ◽  
Northeastern Region ◽  
Medical Institutions

There are no accurate data regarding the prevalence of Chlamydia trachomatis infection in Ukraine. This study aims to estimate the prevalence in the northeastern region of the country through reviewing nucleic acid amplification test results in patients of medical institutions in the Kharkov region during 2014–2016. Samples from 6920 patients (5028 women and 1892 men) aged 12–76 years were tested. The overall positivity rate was 4.5% (95% CI 4.0–5.0): 3.9% (95% CI 3.4–4.5) in women and 6.1% (95% CI 5.1–7.3) in men. The highest prevalence was found in the 16–20 (8.5%, CI 6.3–11.4) and 21–25 (8.0%, CI 6.7–9.4) year age groups. The prevalence in men was higher than in women in all investigated groups. The results show the need for more attention to the prevention, diagnosis, and treatment of chlamydial infection in these age groups of women and men in this region.

scholarly journals Supplementary Testing Is Not Required in the cobas 4800 CT/NG Test for Neisseria gonorrhoeae Weak-Positive Urogenital Samples

2014 ◽  
Vol 53 (1) ◽  
pp. 327-328 ◽  
Author(s):  
Collette Bromhead ◽  
Nadika Liyanarachchy ◽  
Julia Mayes ◽  
Arlo Upton ◽  
Michelle Balm
Keyword(s):  
Nucleic Acid ◽  
Neisseria Gonorrhoeae ◽  
Nucleic Acid Amplification Test ◽  
Pretest Probability ◽  
Nucleic Acid Amplification ◽  
Test Results ◽  
Content Type

Weak-positiveNeisseria gonorrhoeaenucleic acid amplification test results are difficult to interpret. We show that the frequency of unconfirmedN. gonorrhoeaeresults from the cobas 4800 test rises exponentially after 38.0 cycles, where the likelihood of an unconfirmed result exceeds 29%. Supplementary testing of such samples should be avoided; instead, treatment should be based on clinical pretest probability.

The tip of the iceberg: opportunistic screening for Chlamydia trachomatis in asymptomatic patients attending a young people's health clinic reveals a high prevalence — a pilot study

Sexual Health ◽  
2004 ◽  
Vol 1 (2) ◽  
pp. 115 ◽  
Author(s):  
Stephanie Jones ◽  
Sue Barker ◽  
Eugene Athan ◽  
Stephen Graves
Keyword(s):  
Nucleic Acid ◽  
Chlamydia Trachomatis ◽  
Young People ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Chlamydia Infection ◽  
Health Clinic ◽  
Opportunistic Screening ◽  
Sexually Active ◽  
High Prevalence

Objectives: We implemented an opportunistic screening programme for Chlamydia trachomatis amongst patients presenting to a young peoples’ health service in the city of Geelong, Australia, to define the prevalence of infection and to identify specific risk factors. Methods: Over a 7-month period sexually active patients attending the young peoples’ clinic were offered C. trachomatis screening by nucleic acid amplification test. There was 100% acceptance rate among those offered the test. Patient demographics, reason for presentation at the clinic and reported symptoms were documented by the clinicians and correlated with laboratory findings. Results: 163 patients between the ages of 12–25 were tested, nine males and 154 females. The prevalence of chlamydia infection was 5.8% and was highest (16.0%) among patients presenting for the morning after pill. Inhibition of the nucleic acid amplification test occurred in 11.0% of urine samples. All patients with inhibited tests were asked to provide a repeat sample for retesting, but only 50% complied with this request. The majority of repeat samples (88.9%) had no inhibitors present and yielded a negative result. There was no correlation between symptoms and a positive chlamydia result. Conclusions: Chlamydia infection is common in young people engaging in unsafe sexual practice and cannot be predicted by the presence of symptoms. The high prevalence of infection in Geelong would make screening cost effective in this age group. Ongoing population screening of sexually active young people should be encouraged in community health centres. Inhibition of the nucleic acid amplification test was common but repeat testing of urine a few days later usually gave satisfactory results.

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