scholarly journals Low Positive Predictive Value of a Nucleic Acid Amplification Test for NongenitalNeisseria gonorrhoeaeInfection in Homosexual Men

2008 ◽  
Vol 47 (2) ◽  
pp. e25-e27 ◽  
Author(s):  
Leon P. McNally ◽  
David J. Templeton ◽  
Fengyi Jin ◽  
Andrew E. Grulich ◽  
Basil Donovan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Positive Predictive Value ◽  
Predictive Value ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Homosexual Men

scholarly journals Evaluation of the QiagenartusC. difficile QS-RGQ Kit for Detection of Clostridium difficile Toxins A and B in Clinical Stool Specimens

2015 ◽  
Vol 53 (6) ◽  
pp. 1942-1944 ◽  
Author(s):  
Nathalie Jazmati ◽  
Pia Wiegel ◽  
Božica Ličanin ◽  
Georg Plum
Keyword(s):  
Nucleic Acid ◽  
Clostridium Difficile ◽  
Positive Predictive Value ◽  
Negative Predictive Value ◽  
Predictive Value ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Content Type ◽  
Stool Specimens ◽  
Sensitivity Specificity

We compared the QiagenartusC. difficile QS-RGQ kit, a new nucleic acid amplification test for the detection ofClostridium difficiletoxins in stool specimens, with the Cepheid XpertC. difficiletest. The sensitivity, specificity, positive predictive value, and negative predictive value for the QiagenartusC. difficile QS-RGQ test were 100%, 89.5%, 60.9%, and 100%, and those for the Cepheid XpertC. difficiletest were 100%, 90%, 62.2%, and 100%, respectively.

scholarly journals Prospective study to find out the role of gastric aspirate examination by Ziehl-Neelsen staining (ZN staining) and cartridge based nucleic acid amplification test (CB-NAAT) as a diagnostic method in childhood tuberculosis

2018 ◽  
Vol 5 (4) ◽  
pp. 1609
Author(s):  
Akansha Arora ◽  
Anil Jain ◽  
B. S. Karnawat ◽  
Rakesh Kumawat
Keyword(s):  
Nucleic Acid ◽  
Predictive Value ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Mantoux Test ◽  
P Value ◽  
Specific Method ◽  
Gastric Aspirate ◽  
Tuberculosis In Children

Background: Tuberculosis in children has been relatively neglected mainly because clinical diagnosis has low specificity, radiological interpretation is subject to inter-observer variability and the tuberculin skin test is a marker of exposure, not disease. The recent introduction of Cartridge based nucleic acid amplification test has significantly transformed the diagnostics of tuberculosis in adults but its application for Paediatric Tuberculosis is under evaluation. Therefore, authors conducted a study on role of gastric aspirate examination by ZN stain and Cartridge based nucleic acid amplification test in the diagnosis of childhood Tuberculosis.Methods: Authors did a prospective hospital-based study from Nov 2016 to Nov 2017 consisting of 100 randomly selected patients suspected of tuberculosis who had their gastric aspirate tested for CBNAAT and ZN stain for acid fast bacilli (AFB) along with Mantoux test and other routine investigations. Chi square test was used.Results: Culture positive tuberculosis was found in 21 out of 100 children. The sensitivity, specificity, positive predictive value and negative predictive value for CBNAAT were 76.1%, 98.7%, 94.1% and 93.9% and for ZN stain were 47.6%, 98.7%, 90.9% and 87.6% respectively. Positive history of contact (p value 0.0217), reactive Mantoux test (p value < 0.001) and low socioeconomic status were independently associated with a positive CBNAAT result.Conclusions: Analysis of gastric aspirate samples with CBNAAT is a sensitive and specific method for rapid diagnosis of pulmonary tuberculosis in children who cannot produce sputum. Compared with microscopy, CBNAAT offers better sensitivity and its scale up will improve access to tuberculosis diagnostics in children.

scholarly journals Diagnosis of pulmonary tuberculosis with cartridge based nucleic acid amplification test and light emitting diode fluorescent microscopy: a comparative study

2019 ◽  
Vol 6 (5) ◽  
pp. 1580 ◽  
Author(s):  
Pratik Kumar ◽  
Puneet Bhardwaj
Keyword(s):  
Nucleic Acid ◽  
Pulmonary Tuberculosis ◽  
Negative Predictive Value ◽  
Predictive Value ◽  
Light Emitting Diode ◽  
Fluorescent Microscopy ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Smear Microscopy ◽  
Light Emitting

Background: Due to low sensitivity and inability to detect drug resistance, smear microscopy limits its impact on TB control. Culture methods and drug susceptibility testing is complex, time consuming, and takes around 6-8 weeks. A new diagnostic test, cartridge based nucleic acid amplification test (CBNAAT) was developed based on real-time polymerase chain reaction (RT PCR). Objective of this study to compare the results of CBNAAT for diagnosis of pulmonary tuberculosis with LED fluorescent microscopy and sputum culture.Methods: A cross-sectional study was conducted in the department of Chest and TB, CIMS, Bilaspur. Each Sputum sample of presumptive TB patients were tested with CBNAAT, sputum smear  microscopy by light emitting diode (LED) fluorescent microscopy (FM) and solid and liquid culture for diagnosis of Tuberculosis. Results of CBNAAT, Fluorescent Microscopy and Culture for detection of Mycobacterium Tuberculosis were compared.Results: The sensitivity and specificity for CBNAAT were 97% and 100% respectively; while that for Fluorescent microscopy were 70% and 100% respectively. The positive and negative predictive value for CBNAAT was 100% and 96% respectively. The positive and negative predictive value for Fluorescent microscopy was 100% and 73% respectively.Conclusion: CBNAAT is having high sensitivity and specificity for diagnosis of pulmonary tuberculosis. It should be routinely used under national health programme to detect a tuberculosis case efficiently.

scholarly journals Performance of Cartridge Based Nucleic Acid Amplification Test for the Diagnosis of Smear Negative Suspected Tuberculosis - A Study from a Teaching Hospital in Thrissur, Kerala, India

2021 ◽  
Vol 10 (16) ◽  
pp. 1114-1118
Author(s):  
Anupama Sethumadhavan ◽  
Kavitha Paul Konikkara ◽  
Davis Paul
Keyword(s):  
Nucleic Acid ◽  
Predictive Value ◽  
Gold Standard ◽  
Sputum Sample ◽  
False Negative ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
World Health ◽  
Gold Standard Method ◽  
Smear Negative

BACKGROUND In India, everyday more than 6000 people develop tuberculosis (TB) and more than 600 people die of TB (2 death every 5 minutes).1 World Health Organization (WHO) has recently endorsed cartridge based nucleic acid amplification test (CBNAAT) which has the potential to lead a revolution in the diagnosis of tuberculosis. This study intends to assess the performance of CBNAAT for the diagnosis of suspected smear negative pulmonary and extra pulmonary tuberculosis. METHODS The cross-sectional study was carried out in Department of Microbiology, Government Medical College, Thrissur, Kerala, India. CBNAAT was done in district tuberculosis center, Thrissur. The study was done from December 2016 to December 2017. Samples were sent for microscopy, culture and CBNAAT. RESULTS A total of 250 patients were evaluated. Majority of the specimens collected were sputum (61.2 %) followed by bronchial wash (17.6 %). Culture was positive in 48 specimens. CBNAAT was positive in 58 specimens. Both culture and CBNAAT were positive in 47 patients. CBNAAT was negative in 1 specimen but positive in culture test. CBNAAT detected an additional 10 samples. Taking culture as gold standard, culture positives were taken as true positives and culture negatives were taken as true negatives. Accordingly, true positive was 48, true negative was 202, false positive was 10 and false negative was 1. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were respectively 97.95 %, 95.2 %, 82.75 % and 99.5 %. CBNAAT missed out a sputum sample which was culture positive. CONCLUSIONS We found CBNAAT to be an important diagnostic modality especially in smear negative patients for early diagnosis and treatment of TB. Culture of mycobacteria is considered as a gold standard method, but it takes weeks to obtain a positive result and simultaneous detection of rifampicin resistance is not possible with this method. KEY WORDS Tuberculosis, Smear Negative TB, ZN Stain, AFB, Petroff’s Method, CBNAAT, RNTCP, Culture

Evaluation of anopagene-based nucleic acid amplification test for detection ofNeisseria gonorrhoeaein urogenital samples in North India

2012 ◽  
Vol 140 (11) ◽  
pp. 2110-2116 ◽  
Author(s):  
R. VERMA ◽  
S. SOOD ◽  
M. BALA ◽  
N. MAHAJAN ◽  
A. KAPIL ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Positive Predictive Value ◽  
Predictive Value ◽  
Nucleic Acid Amplification ◽  
North India ◽  
Rrna Gene ◽  
New Delhi ◽  
Sensitivity Specificity ◽  
Low Prevalence ◽  
Pcr Targeting

SUMMARYDue to the poor positive predictive value of nucleic acid amplification tests (NAATs) for gonorrhoea when applied to a low-prevalence setting, current guidelines recommend the use of supplementary polymerase chain reaction (PCR) targeting a different gene for confirmation of true positives in urogenital specimens. This study sought to standardize and evaluate performance of an in-houseopagene-based PCR assay for gonorrhoea compared to assays targeting theporApseudogene and 16S rRNA gene. Four hundred samples (300 endocervical, 100 urethral swabs) from patients attending STD clinics in New Delhi, India were used. The sensitivity, specificity, positive predictive value and negative predictive value of theopa-based PCR were 100%, 97·9%, 89·5% and 100%, respectively. In females, the use of NAATs provided enhanced diagnosis of gonorrhoea.

scholarly journals A Retrospective Cross-Sectional Study of the Utility of Cartridge-Based Nucleic Acid Amplification Test in Diagnosis of Pulmonary and Extrapulmonary Tuberculosis in People Living with HIV in Second Highest HIV Prevalent State in India

2021 ◽  
Author(s):  
Tade Bagbi ◽  
Ningthoukhongjam Reema ◽  
S. Bhagyabati Devi ◽  
Thangjam Gautam Singh ◽  
Mohammad Jaleel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Cross Sectional Study ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Rifampicin Resistance ◽  
Sputum Smear ◽  
Smear Microscopy ◽  
Sectional Study ◽  
Cross Sectional ◽  
Smear Negative

Abstract Introduction Tuberculosis (TB) in people living with human immunodeficiency virus (PLHIV) is difficult to diagnose due to fewer organisms in sputum and extrapulmonary samples. Sputum culture takes 4 to 8 weeks for growth of the mycobacteria. Delayed treatment for TB in PLHIV leads to increased mortality. This study evaluated cartridge-based nucleic acid amplification test (CBNAAT) as a diagnostic tool for diagnosis of pulmonary TB (PTB) and extrapulmonary TB (EPTB) in PLHIV in the second most HIV prevalent state in India and for comparing its efficacy between Ziehl–Neelsen (ZN) staining sputum smear–positive and sputum smear–negative TB. Methods This cross-sectional study was conducted in RIMS, Imphal, with 167 PLHIV patients, age 15 years or older, having signs and symptoms of TB. Appropriate samples for sputum microscopy and CBNAAT were sent. Conclusion The overall sensitivity of sputum smear for acid-fast bacillus (AFB) was found to be 30.71% and that of CBNAAT was 38.57%. Sensitivity of CBNAAT for sputum smear–positive and sputum smear–negative TB was 100 and 11.3%, respectively. Sensitivity of ZN smear for AFB of EPTB sample was 48.1% and that of CBNAAT was 59.25%. In both PTB and EPTB, CBNAAT showed an increase in diagnosis of microbiologically confirmed PTB cases by 7.8 and 11.1%, respectively, over and above the cases diagnosed by ZN smear microscopy. Rifampicin resistance was detected in five patients. We conclude that CBNAAT is a rapid test with better sensitivity in diagnosis of PTB and EPTB in PLHIV, compared with ZN smear microscopy. It detects rifampicin resistance for multidrug-resistant TB and helps in early treatment intervention.

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