scholarly journals Comparative Analysis of CD8+T Cell Responses against Human Cytomegalovirus Proteins pp65 and Immediate Early 1 Shows Similarities in Precursor Frequency, Oligoclonality, and Phenotype

2002 ◽  
Vol 185 (8) ◽  
pp. 1025-1034 ◽  
Author(s):  
Naeem Khan ◽  
Mark Cobbold ◽  
Russell Keenan ◽  
Paul A. H. Moss
Keyword(s):  
Comparative Analysis ◽  
T Cell ◽  
Human Cytomegalovirus ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Immediate Early ◽  
Cell Responses ◽  
Precursor Frequency

scholarly journals Immediate-Early Expression of a Recombinant Antigen by Modified Vaccinia Virus Ankara Breaks the Immunodominance of Strong Vector-Specific B8R Antigen in Acute and Memory CD8 T-Cell Responses

2010 ◽  
Vol 84 (17) ◽  
pp. 8743-8752 ◽  
Author(s):  
Karen Baur ◽  
Kay Brinkmann ◽  
Marc Schweneker ◽  
Juliane Pätzold ◽  
Christine Meisinger-Henschel ◽  
...  
Keyword(s):  
T Cell ◽  
Vaccinia Virus ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Immediate Early ◽  
Egfp Expression ◽  
Cell Responses ◽  
Modified Vaccinia Virus Ankara ◽  
Memory Cd8 T Cell ◽  
Early Late

ABSTRACT Efficient T-cell responses against recombinant antigens expressed by vaccinia virus vectors require expression of these antigens in the early phase of the virus replication cycle. The kinetics of recombinant gene expression in poxviruses are largely determined by the promoter chosen. We used the highly attenuated modified vaccinia virus Ankara (MVA) to determine the role of promoters in the induction of CD8 T-cell responses. We constructed MVA recombinants expressing either enhanced green fluorescent protein (EGFP) or chicken ovalbumin (OVA), each under the control of a hybrid early-late promoter (pHyb) containing five copies of a strong early element or the well-known early-late p7.5 or pS promoter for comparison. In primary or cultured cells, EGFP expression under the control of pHyb was detected within 30 min, as an immediate-early protein, and remained higher over the first 6 h of infection than p7.5- or pS-driven EGFP expression. Repeated immunizations of mice with recombinant MVA expressing OVA under the control of the pHyb promoter led to superior acute and memory CD8 T-cell responses compared to those to p7.5- and pS-driven OVA. Moreover, OVA expressed under the control of pHyb replaced the MVA-derived B8R protein as the immunodominant CD8 T-cell antigen after three or more immunizations. This is the first demonstration of an immediate-early neoantigen expressed by a poxviral vector resulting in superior induction of neoantigen-specific CD8 T-cell responses.

Human cytomegalovirus-specific CD4+ and CD8+ T cell responses in primary infection of the immunocompetent and the immunocompromised host

2009 ◽  
Vol 131 (3) ◽  
pp. 395-403 ◽  
Author(s):  
Daniele Lilleri ◽  
Paola Zelini ◽  
Chiara Fornara ◽  
Giuditta Comolli ◽  
Maria Grazia Revello ◽  
...  
Keyword(s):  
T Cell ◽  
Human Cytomegalovirus ◽  
Primary Infection ◽  
T Cell Responses ◽  
Immunocompromised Host ◽  
Cd8 T Cell ◽  
Cell Responses

scholarly journals DNA Immunization Using Highly Conserved Murine Cytomegalovirus Genes Encoding Homologs of Human Cytomegalovirus UL54 (DNA Polymerase) and UL105 (Helicase) Elicits Strong CD8 T-Cell Responses and Is Protective against Systemic Challenge

2007 ◽  
Vol 81 (14) ◽  
pp. 7766-7775 ◽  
Author(s):  
Christopher S. Morello ◽  
Laura A. Kelley ◽  
Michael W. Munks ◽  
Ann B. Hill ◽  
Deborah H. Spector
Keyword(s):  
T Cells ◽  
T Cell ◽  
Dna Polymerase ◽  
Human Cytomegalovirus ◽  
Cd8 T Cells ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Murine Cytomegalovirus ◽  
Dna Immunization ◽  
Cell Responses

ABSTRACT Human cytomegalovirus (HCMV) establishes a lifelong infection with the potential for reinfection or viral transmission even in the presence of strong and diverse CD8 T-lymphocyte responses. This suggests that the CMVs skew the host T-cell response in order to favor viral persistence. In this study, we hypothesized that the essential, nonstructural proteins that are highly conserved among the CMVs may represent a novel class of T-cell targets for vaccine-mediated protection due to their requirements for expression and sequence stability, but that the observed subdominance of these antigens in the CMV-infected host results from the virus limiting the T-cell responses to otherwise-protective specificities. We found that DNA immunization of mice with the murine CMV (MCMV) homologs of HCMV DNA polymerase (M54) or helicase (M105) was protective against virus replication in the spleen following systemic challenge, with the protection level elicited by the M54 DNA being comparable to that of DNA expressing the immunodominant IE1 (pp89). Intracellular gamma interferon staining of CD8 T cells from mice immunized with either the M54 or M105 DNAs showed strong primary responses that recalled rapidly after viral challenge. M54- and M105-specific CD8 T cells were detected after the primary MCMV infection, but their levels were not consistently above the background level. The conserved, essential proteins of the CMVs thus represent a novel class of CD8 T-cell targets that may contribute to a successful HCMV vaccine strategy.

scholarly journals Human cytomegalovirus microRNA miR-US4-1 inhibits CD8+ T cell responses by targeting the aminopeptidase ERAP1

2011 ◽  
Vol 12 (10) ◽  
pp. 984-991 ◽  
Author(s):  
Sungchul Kim ◽  
Sanghyun Lee ◽  
Jinwook Shin ◽  
Youngkyun Kim ◽  
Irini Evnouchidou ◽  
...  
Keyword(s):  
T Cell ◽  
Human Cytomegalovirus ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Cell Responses

Ex vivo monitoring of human cytomegalovirus-specific CD8+ T-cell responses using QuantiFERON�-CMV

2007 ◽  
Vol 9 (2) ◽  
pp. 165-170 ◽  
Author(s):  
S. Walker ◽  
C. Fazou ◽  
T. Crough ◽  
R. Holdsworth ◽  
P. Kiely ◽  
...  
Keyword(s):  
T Cell ◽  
Human Cytomegalovirus ◽  
Ex Vivo ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Cell Responses
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