Gametophytes of Botrychium dissectum as Grown in Sterile Culture

1972 ◽  
Vol 133 (3) ◽  
pp. 336-339 ◽  
Author(s):  
Dean P. Whittier
Keyword(s):  
Sterile Culture

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

scholarly journals Study of drug sensitivity profile of patients with throat infections in lower Himalayan region

2020 ◽  
Vol 6 (4) ◽  
pp. 754
Author(s):  
Manjeet Singh
Keyword(s):  
Developing Countries ◽  
Drug Sensitivity ◽  
Himalayan Region ◽  
Benzyl Penicillin ◽  
Major Health Problem ◽  
Major Health ◽  
Sterile Culture ◽  
Culture Sensitivity ◽  
Gram Negative Organisms ◽  
Sensitivity Profile

<p class="abstract"><strong>Background:</strong> Infections of throat is a major health problem in developing countries. It can cause significant complications and morbidity if not addressed on time. The aim of the study was to study the drug sensitivity profile of patients with throat infections in lower Himalayan region.</p><p class="abstract"><strong>Methods:</strong> 100 patients with throat infections or sore throat were included in the study. Throat samples collected through a sterile culture sensitivity tube and sent to microbiological lab in the Regional Hospital Bilaspur (SRL diagnostics).  </p><p class="abstract"><strong>Results:</strong> Gram positive organisms were resistant to benzyl penicillin or ampicillin, clindamycin, ciprofloxacin or levofloxacin, trimethoprim or sulphamethoxazole and sensitive to tetracycline, gentamicin and linezolid. Gram negative organisms were resistant to ampicillin, amoxyclav and sensitive to pipracillin or tazobactum, cefoperazone, amikacin, gentamicin, imipenem, cefepime, ciprofloxacin and trimethoprim or sulphamethoxazole.</p><p class="abstract"><strong>Conclusions:</strong> Throat infections along with emerging drug resistance are still a major health in developing countries.</p>

Reproductive success in a reintroduced population of a critically endangered shrub, Symonanthus bancroftii (Solanaceae)

2007 ◽  
Vol 55 (4) ◽  
pp. 425 ◽  
Author(s):  
Qigang Ye ◽  
Eric Bunn ◽  
Siegfried L. Krauss ◽  
Kingsley W. Dixon
Keyword(s):  
Reproductive Success ◽  
Pollen Viability ◽  
Natural Habitat ◽  
Female Parent ◽  
Clonal Plants ◽  
Critically Endangered ◽  
Positive Sign ◽  
Male And Female ◽  
Sterile Culture

Symonanthus bancroftii is a critically endangered dioecious shrub, with only one male and one female plant known in the wild. Micropropagated, clonal plants of the male and female parents were hand-pollinated. Seed was germinated in sterile culture and a subsequent daughter (F1) seedling was multiplied in vitro. Micropropagated plants of the original parents and daughter were reintroduced to a natural habitat site. We detail the first study of pollination biology and reproductive success in this extremely rare species. Floral morphology, flowering phenology, pollen viability, female receptivity and fruit set of reintroduced plants of S. bancroftii were investigated. Flower viability of S. bancroftii shows a 25-day maximum for male flowers, whereas non-pollinated female flowers remain viable for a maximum of 38 days (female parent) and 41 days (daughter), respectively. Flowering of reintroduced male and female plants overlaps from mid-June to early November. Pollen remained viable for at least 8 days after anther dehiscence. Maximum stigma longevity recorded was 28 days (female parent) and 39 days (daughter) and maximum ovule longevity varied from 13 days (female parent) to 28 days (daughter). The mean percentage of flowers setting fruit was 39 ± 13% for female parent plants and 48 ± 4% for daughter plants. Empirical reproductive success measures for male–daughter crosses generally exceeded those of the original parent crosses. The reproductive success of S. bancroftii so soon after reintroduction of plants is a positive sign indicating that rapid accumulation of a soil seedbank is feasible and is thus an important first step towards successful establishment of self-sustaining populations of this critically endangered species.

Effects of Exogenous Sucrose Feeding on Photosynthesis in the C3 Plant Tobacco and the C4 Plant Flaveria bidentis

1997 ◽  
Vol 24 (3) ◽  
pp. 291 ◽  
Author(s):  
Robert T. Furbank ◽  
Jeni Pritchard ◽  
Colin L.D. Jenkins
Keyword(s):  
Gene Expression ◽  
Leaf Area ◽  
Experimental System ◽  
Threshold Level ◽  
C3 Plant ◽  
Sterile Culture ◽  
Unit Leaf ◽  
Photosynthetic Genes ◽  
Sucrose Feeding ◽  
Flaveria Bidentis

Seeds of the C4 dicot Flaveria bidentis and the C3 dicot Nicotiana tabacum were germinated on expanded cotton plugs and grown for 28–35 days in a sterile culture system with liquid nutrients supplied to the roots in the presence or absence of 5% (w/v) sucrose. Despite previous reports that supplying sucrose exogenously to plants inhibits expression of photosynthetic genes, sucrose application in this system stimulated maximum photosynthetic rates and these were comparable to those measured in glasshouse-grown material. The activities of many photosynthetic enzymes in the youngest fully expanded leaves of both Flaveria and tobacco plants were increased by sucrose feeding. Sucrose levels were increased more than 2-fold in tobacco and 3-fold in Flaveria leaves (on a leaf area basis) by sucrose application, and both hexose and starch levels also increased. Chlorophyll per unit leaf area in sucrose-fed plants increased by approximately 20% in tobacco and 2-fold in Flaveria. There was no evidence of sucrose repression of photosynthetic competence in this experimental system. We propose that, over the concentration range present in the leaves in these experiments, sucrose acts as a positive signal for photosynthetic gene expression but that, above a threshold level, inhibition of gene expression seen in other studies could still occur.

Experiments on the Control of Fertility of Fern Leaves in Sterile Culture

1958 ◽  
Vol 119 (4) ◽  
pp. 203-208 ◽  
Author(s):  
I. M. Sussex ◽  
Taylor A. Steeves
Keyword(s):  
Sterile Culture ◽  
Fern Leaves

Dormancy regulation: hormonal interaction in maple (Acer platanoides)

1969 ◽  
Vol 47 (7) ◽  
pp. 1165-1169 ◽  
Author(s):  
A. E. DeMaggio ◽  
J. A. Freeberg
Keyword(s):  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Acid Treatment ◽  
Acer Platanoides ◽  
Sterile Culture ◽  
Hormonal Interaction ◽  
Bud Growth ◽  
Short Days ◽  
Gibberellic Acid Treatment

Whole buds and excised apices (bud scales removed) from dormant trees of Acer platanoides grow in sterile culture in long days but have different responses to gibberellic acid treatment. Bud growth is stimulated by the hormone in long and short days but apices are unaffected. Abscisic acid inhibits not only gibberellin-stimulated bud growth but also the photoperiodically stimulated growth of apices.

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