Experiments on the Control of Fertility of Fern Leaves in Sterile Culture

1958 ◽  
Vol 119 (4) ◽  
pp. 203-208 ◽  
Author(s):  
I. M. Sussex ◽  
Taylor A. Steeves
Keyword(s):  
Sterile Culture ◽  
Fern Leaves

Meiosis and Sporogenesis in Excised Fern Leaves Grown in Sterile Culture

1965 ◽  
Vol 126 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Mary E. Clutter ◽  
Ian M. Sussex
Keyword(s):  
Sterile Culture ◽  
Fern Leaves

Growth of Excised Fern Leaves in Sterile Culture

Nature ◽  
1953 ◽  
Vol 172 (4379) ◽  
pp. 624-625 ◽  
Author(s):  
I. M. SUSSEX ◽  
TAYLOR A. STEEVES
Keyword(s):  
Sterile Culture ◽  
Fern Leaves

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

Gametophytes of Botrychium dissectum as Grown in Sterile Culture

1972 ◽  
Vol 133 (3) ◽  
pp. 336-339 ◽  
Author(s):  
Dean P. Whittier
Keyword(s):  
Sterile Culture

scholarly journals Study of drug sensitivity profile of patients with throat infections in lower Himalayan region

2020 ◽  
Vol 6 (4) ◽  
pp. 754
Author(s):  
Manjeet Singh
Keyword(s):  
Developing Countries ◽  
Drug Sensitivity ◽  
Himalayan Region ◽  
Benzyl Penicillin ◽  
Major Health Problem ◽  
Major Health ◽  
Sterile Culture ◽  
Culture Sensitivity ◽  
Gram Negative Organisms ◽  
Sensitivity Profile

<p class="abstract"><strong>Background:</strong> Infections of throat is a major health problem in developing countries. It can cause significant complications and morbidity if not addressed on time. The aim of the study was to study the drug sensitivity profile of patients with throat infections in lower Himalayan region.</p><p class="abstract"><strong>Methods:</strong> 100 patients with throat infections or sore throat were included in the study. Throat samples collected through a sterile culture sensitivity tube and sent to microbiological lab in the Regional Hospital Bilaspur (SRL diagnostics).  </p><p class="abstract"><strong>Results:</strong> Gram positive organisms were resistant to benzyl penicillin or ampicillin, clindamycin, ciprofloxacin or levofloxacin, trimethoprim or sulphamethoxazole and sensitive to tetracycline, gentamicin and linezolid. Gram negative organisms were resistant to ampicillin, amoxyclav and sensitive to pipracillin or tazobactum, cefoperazone, amikacin, gentamicin, imipenem, cefepime, ciprofloxacin and trimethoprim or sulphamethoxazole.</p><p class="abstract"><strong>Conclusions:</strong> Throat infections along with emerging drug resistance are still a major health in developing countries.</p>

Correlation between lanthanide contents in fern leaves

1997 ◽  
Vol 58 (1-4) ◽  
pp. 133-141 ◽  
Author(s):  
Jitsuya Takada ◽  
Kazuo Nishimura ◽  
Teruie Sumino ◽  
Yoshiko Tanaka ◽  
Mitsuhiko Akaboshi
Keyword(s):  
Fern Leaves

Reproductive success in a reintroduced population of a critically endangered shrub, Symonanthus bancroftii (Solanaceae)

2007 ◽  
Vol 55 (4) ◽  
pp. 425 ◽  
Author(s):  
Qigang Ye ◽  
Eric Bunn ◽  
Siegfried L. Krauss ◽  
Kingsley W. Dixon
Keyword(s):  
Reproductive Success ◽  
Pollen Viability ◽  
Natural Habitat ◽  
Female Parent ◽  
Clonal Plants ◽  
Critically Endangered ◽  
Positive Sign ◽  
Male And Female ◽  
Sterile Culture

Symonanthus bancroftii is a critically endangered dioecious shrub, with only one male and one female plant known in the wild. Micropropagated, clonal plants of the male and female parents were hand-pollinated. Seed was germinated in sterile culture and a subsequent daughter (F1) seedling was multiplied in vitro. Micropropagated plants of the original parents and daughter were reintroduced to a natural habitat site. We detail the first study of pollination biology and reproductive success in this extremely rare species. Floral morphology, flowering phenology, pollen viability, female receptivity and fruit set of reintroduced plants of S. bancroftii were investigated. Flower viability of S. bancroftii shows a 25-day maximum for male flowers, whereas non-pollinated female flowers remain viable for a maximum of 38 days (female parent) and 41 days (daughter), respectively. Flowering of reintroduced male and female plants overlaps from mid-June to early November. Pollen remained viable for at least 8 days after anther dehiscence. Maximum stigma longevity recorded was 28 days (female parent) and 39 days (daughter) and maximum ovule longevity varied from 13 days (female parent) to 28 days (daughter). The mean percentage of flowers setting fruit was 39 ± 13% for female parent plants and 48 ± 4% for daughter plants. Empirical reproductive success measures for male–daughter crosses generally exceeded those of the original parent crosses. The reproductive success of S. bancroftii so soon after reintroduction of plants is a positive sign indicating that rapid accumulation of a soil seedbank is feasible and is thus an important first step towards successful establishment of self-sustaining populations of this critically endangered species.

Sign in / Sign up

Export Citation Format

Share Document