Persistence of Cytoplasmic Differentiation during Mitosis

1937 ◽  
Vol 71 (737) ◽  
pp. 605-609 ◽  
Author(s):  
Alden B. Dawson
Keyword(s):  
Cytoplasmic Differentiation

Nuclear and cytoplasmic differentiation in developing sperm of the crayfish, Cambaroides japonicus

1961 ◽  
Vol 53 (2) ◽  
pp. 141-158 ◽  
Author(s):  
G. Yasuzumi ◽  
G. I. Kaye ◽  
G. D. Pappas ◽  
H. Yamamoto ◽  
I. Tsubo
Keyword(s):  
Cytoplasmic Differentiation

scholarly journals Automatic cell identification and enrichment in lung cancer. II. Acridine orange for cell sorting of sputum.

1979 ◽  
Vol 27 (1) ◽  
pp. 552-556 ◽  
Author(s):  
H W Tyrer ◽  
J F Golden ◽  
M H Vansickel ◽  
C K Echols ◽  
J K Frost ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Propidium Iodide ◽  
Cell Sorting ◽  
Fluorescence Spectra ◽  
Fluorescent Dyes ◽  
Appreciable Amount ◽  
Pleural Effusions ◽  
Green Fluorescence ◽  
Total Fluorescence ◽  
Cytoplasmic Differentiation

Fluorescence spectra were obtained from cells from sputum and pleural effusions stained with different fluorescent dyes and fixed by alternate methods. The spectra were referenced to a standard allowing for fluorescence comparisons of unstained and stained cells under various conditions. The metachromasia of acridine orange-stained cells offers nuclear/cytoplasmic differentiation in a single stain; mithramycin and propidium iodide do not. Unstained cells have an appreciable amount of green (546 nm) fluorescence, as does Carbowax in Saccomanno's preservative. Cytoplasm stained with acidine orange also has appreciable green fluorescence. Consequently, cells with much cytoplasm have high total fluorescence. Cytoplasmic fluorescence is negligible with mithramycin or propidium iodide. The metachromasia of acridine orange-stained cells is altered by alcohol and Carbowax levels in fixatives, keeping other factors constant.

scholarly journals Clonal Proliferation and Cytokine Requirement of Murine Progenitors for Natural Killer Cells

Blood ◽  
1997 ◽  
Vol 89 (11) ◽  
pp. 4005-4012 ◽  
Author(s):  
Yuichi Aiba ◽  
Fumiya Hirayama ◽  
Makio Ogawa
Keyword(s):  
Natural Killer ◽  
Culture System ◽  
Nk Cell ◽  
Cell Development ◽  
Cell Culture System ◽  
Fetal Thymus ◽  
Steel Factor ◽  
Clonal Proliferation ◽  
Interleukin 7 ◽  
Cytoplasmic Differentiation

Abstract We have established a clonal cell culture system that supports the proliferation of committed natural killer (NK) cell progenitors of mice to investigate the pathway and cytokine regulation of NK cell development. Day 14 fetal thymocytes cultured in methylcellulose with interleukin-7 (IL-7), IL-15, and steel factor (SF ) formed diffuse colonies that could not be classified to known colony types. Single-cell origin of the colonies was established by micromanipulation of the colony-forming cells. Cells in the colonies are very blastic, showing no cytoplasmic differentiation, and express Ly5, Thy-1, and CD25 but not myeloid, B, mature T, or NK cell markers. The cells lack T, B, and myeloid potentials but can differentiate to mature NK cells in fetal thymus organ culture, suggesting that the colonies consist of NK committed progenitors. Examination of the minimal cytokine requirement for the NK colony formation showed that IL-7 and SF are indispensable for the formation of immature NK cell colonies. Both IL-2 and IL-15 increased the frequency of colonies. In contrast to IL-2, IL-7, and IL-15, IL-4 strongly inhibited the formation of the colonies. This quantitative clonal culture will provide a useful means to examine the mechanism of NK cell development.

Development of megakaryocytes in bone marrow of the rat: an analysis by electron microscopy and high resolution autoradiography

1971 ◽  
Vol 177 (1047) ◽  
pp. 265-274 ◽  
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
High Resolution ◽  
The Other ◽  
Small Cells ◽  
Tubule Formation ◽  
Dense Granules ◽  
Microscopic Studies ◽  
High Resolution Autoradiography ◽  
Cytoplasmic Differentiation

The fine structure of immature megakaryocytes was studied by the use of high resolution autoradiography to identify DNA synthesizing cells. Approximately 8.5 % of all megakaryocytes were labelled, and these cells were of three main types. The most immature were small cells with bilobed nuclei and sparse cytoplasm. Demarcation tubule formation from the cell surface was seen in these cells. The other two types of cell had three or more nuclear lobes, and a greater amount of cytoplasm containing dense granules and demarcation tubules. In the most mature labelled cells, tubules were numerous. Microtubules were seen in some labelled cells. Megakaryocytes in mitosis had similar cytoplasmic characteristics, with dense granules and demarcation tubules. No cells corresponding to the megakaryoblast described in light microscopic studies were observed. Possible reasons for this finding are discussed, and it is suggested that the megakaryocyte possesses a lobulated nucleus and shows cytoplasmic differentiation from the time of its first endomitosis.

Observations on chick-embryo lens histogenesis in vivo and in vitro

1974 ◽  
Vol 52 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Brian G. McLean ◽  
Cyril V. Finnegan
Keyword(s):  
Chick Embryo ◽  
Cell Elongation ◽  
Fiber Cell ◽  
Cell Extension ◽  
Differentiated Cells ◽  
Cytoplasmic Organization ◽  
Extensive Cell ◽  
Cytoplasmic Differentiation

The 6-day chick-embryo lens anterior epithelium will undergo histogenesis in vitro. A 'normal' epithelial curvature in vitro is required for extensive fiber-cell elongation; a reversed curvature, or flat epithelial conformation, results in considerably reduced cell extension. With a 'normal' epithelial curvature, cell elongation in vitro progresses rapidly for about 3 days, and changes in cytoplasmic organization (ultrastructure) take place that are similar to those associated with fiber-cell differentiation in vivo. However, these cells do not achieve as full a cytoplasmic differentiation as was observed in the most differentiated cells of the normal embryonic lens. Epithelia explanted with a reversed curvature, while evidencing reduced cell elongation, had, after 3 days in vitro, differentiated (according to criteria of ultrastructure and relative proportions of soluble proteins) in a manner similar to those epithelia explanted with a 'normal' curvature and in which extensive cell elongation had been demonstrated.

Cytoplasmic differentiation using near-isonuclear polycytoplasmic male sterile lines in pearl millet

Euphytica ◽  
1993 ◽  
Vol 67 (1-2) ◽  
pp. 127-134 ◽  
Author(s):  
D. S. Virk ◽  
J. S. Brar ◽  
B. K. Mangat
Keyword(s):  
Pearl Millet ◽  
Male Sterile ◽  
Cytoplasmic Differentiation
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