Cytoplasmic differentiation and dedifferentiation in the fungusAllomyces

PROTOPLASMA ◽  
1961 ◽  
Vol 54 (3) ◽  
pp. 323-327 ◽  
Author(s):  
Gilbert Turian
Keyword(s):  
Differentiation And Dedifferentiation ◽  
Cytoplasmic Differentiation

scholarly journals Author Correction: Alternating Differentiation and Dedifferentiation between Mature Osteoblasts and Osteocytes

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Naruhiko Sawa ◽  
Hiroki Fujimoto ◽  
Yoshihiko Sawa ◽  
Junro Yamashita
Keyword(s):  
Differentiation And Dedifferentiation

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Interspecific differences in differentiation and dedifferentiation patterns in the genusNicotiana

1985 ◽  
Vol 151 (1-2) ◽  
pp. 19-29 ◽  
Author(s):  
P. Bogani ◽  
M. Buiatti ◽  
S. Tegli ◽  
M. G. Pellegrini ◽  
P. Bettini ◽  
...  
Keyword(s):  
Interspecific Differences ◽  
Differentiation And Dedifferentiation

scholarly journals THE EFFECT OF COLCHICINE ON MYOGENESIS IN VIVO IN RANA PIPIENS AND RHODNIUS PROLIXUS (HEMIPTERA)

1968 ◽  
Vol 39 (3) ◽  
pp. 544-555 ◽  
Author(s):  
Robert H. Warren
Keyword(s):  
Cell Shape ◽  
Rana Pipiens ◽  
Colchicine Treatment ◽  
Rhodnius Prolixus ◽  
Microtubule Disruption ◽  
Blood Sucking ◽  
Tadpole Tail ◽  
Differentiation And Dedifferentiation ◽  
Regeneration Blastema

The effect of colchicine on myogenesis in vivo has been studied in the regenerating tadpole tail of the frog, Rana pipiens, and in the abdominal molting muscles of a blood-sucking bug, Rhodnius prolixus Stål. Colchicine is shown to disrupt microtubules in the differentiating muscle cells of both these organisms. The disruption of microtubules is correlated with a loss of longitudinal anisometry in the myoblasts and myotubes of the regeneration blastema in the tadpole tail. Before colchicine treatment, the myotubes contain longitudinally oriented myofibrils. After colchicine treatment, rounded, multinucleate myosacs containing randomly oriented myofibrils are present. It is suggested that the primary function of microtubules in myogenesis in the Rana pipiens tadpole is the maintenance of cell shape. The abdominal molting muscles of Rhodnius undergo repeated phases of differentiation and dedifferentiation of the sarcoplasm. However, the longitudinal anisometry of the muscle fibers is maintained in all phases by the attachments of the ends of the fibers to the exoskeleton, and microtubule disruption does not alter cell shape. The orientation of the developing myofibrils is also unaltered, indicating that the microtubules do not directly align or support the myofibrils in this system.

Nuclear and cytoplasmic differentiation in developing sperm of the crayfish, Cambaroides japonicus

1961 ◽  
Vol 53 (2) ◽  
pp. 141-158 ◽  
Author(s):  
G. Yasuzumi ◽  
G. I. Kaye ◽  
G. D. Pappas ◽  
H. Yamamoto ◽  
I. Tsubo
Keyword(s):  
Cytoplasmic Differentiation

scholarly journals Alternating Differentiation and Dedifferentiation between Mature Osteoblasts and Osteocytes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Naruhiko Sawa ◽  
Hiroki Fujimoto ◽  
Yoshihiko Sawa ◽  
Junro Yamashita
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Bone Matrix ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Two Dimensional ◽  
Gene Manipulation ◽  
3D Cultures ◽  
Cycle Arrest ◽  
Differentiation And Dedifferentiation

AbstractOsteocytes are terminally differentiated osteoblasts embedded in the bone matrix. Evidence indicates that cells in the mesenchymal lineage possess plasticity. However, whether or not osteocytes have the capacity to dedifferentiate back into osteoblasts is unclear. This study aimed to clarify the dedifferentiation potential of osteocytes. Mouse calvarial osteoblasts were isolated and maintained in normal two-dimensional (2D) or collagen gel three-dimensional (3D) cultures. In 2D cultures, osteoblasts exhibited a typical fibroblast-like shape with high Alpl and minimal Sost, Fgf23, and Dmp1 expression and osteoblasts formed mineralised nodules. When these osteoblasts were transferred into 3D cultures, they showed a stellate shape with diminished cytoplasm and numerous long processes and expression of Alpl decreased while Sost, Fgf23, and Dmp1 were significantly increased. These cells were in cell cycle arrest and showed suppressed mineralisation, indicating that they were osteocytes. When these osteocytes were recovered from 3D cultures and cultured two-dimensionally again, they regained adequate cytoplasm and lost the long processes, resulting in a fibroblast-like shape. These cells showed high Alpl and low Sost, Fgf23, and Dmp1 expression with a high mineralisation capability, indicating that they were osteoblasts. This report shows that osteocytes possess the capacity to dedifferentiate back into mature osteoblasts without gene manipulation.

P3503Transcriptome changes in atrial myocytes during the transition from a proliferative into a contractile phenotype and vice versa

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
J Liu ◽  
R Tsonaka ◽  
H Mei ◽  
B Akerboom ◽  
M Schalij ◽  
...  
Keyword(s):  
Stem Cells ◽  
Rna Sequencing ◽  
Cardiac Contraction ◽  
Neonatal Rat ◽  
Cardiomyocyte Differentiation ◽  
Atrial Myocytes ◽  
Contractile Phenotype ◽  
Cardiomyogenic Differentiation ◽  
Differentiation And Proliferation ◽  
Differentiation And Dedifferentiation

Abstract Background iAM-1 cells are conditionally immortalized neonatal rat atrial myocytes allowing toggling between proliferative and contractile phenotypes by a single-component change in culture medium composition. In the absence of proliferation stimuli, the cells synchronously differentiate into functional cardiomyocytes. Following re-expression of the immortalization factor, the fully differentiated iAM-1 cells dedifferentiate and start to proliferate again. Purpose The aim of our study was to investigate the changes in gene expression profile in iAM-1 cells during one round of cardiac differentiation and dedifferentiation in order to identify potential (new) regulators of atrial myocyte differentiation and proliferation. Methods RNA sequencing was performed on iAM-1 cells at 9 time points during one cycle of cardiomyogenic differentiation and dedifferentiation (20 million 150-bp paired-end reads per sample, 4 samples per time point). The resulting sequence data were analysed by EdgeR. Hierarchical clustering and principle component analysis were performed in R. GO category enrichment was determined using DAVID. Results Approximately 13,000 genes were extracted from the RNA sequencing analysis. In general, dynamic changes in mRNA levels during the transition from a proliferative into a contractile phenotype opposed those that occurred when differentiated iAM-1 were re-exposed to proliferation stimuli. These inverse trends were most evident for genes involved in cell cycle progression, DNA replication, sarcomere formation and cardiac contraction. Moreover, the RNA-SEQ data allowed us to make a distinction between genes contributing to the early and late phases of cardiomyogenic differentiation and dedifferentiation and to identify similarities and differences in the transcriptional programs underlying the cardiomyogenic differentiation of iAM-1 cells versus those of embryonic stem cells and induced pluripotent stem cells. The transcriptome analysis also unveiled several genes with potentially important and previously unrecognized roles in cardiomyocyte differentiation and proliferation. iAM-1 differentiation and dedifferention Conclusions Due to their ability to homogenously and synchronously differentiate and dedifferentiate, iAM-1 cells offer unique new insights into the transcriptional regulation of cardiomyocyte differentiation and proliferation.

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