scholarly journals Quantitative studies on the polarization optical properties of living cells. I. Microphotometric birefringence detection system.

1981 ◽  
Vol 89 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Y Hiramoto ◽  
Y Hamaguchi ◽  
Y Shôji ◽  
S Shimoda

A method of polarization optical analysis is described in which phase retardation attributable to birefringence of a minute area in a microscopic object is determined. The optical system consists of a polarizing microscope with "rectified" strain-free lenses, a photoelectric detector to determine the intensity of the light passing through a minute window located at the image plane of the specimen, and a stage that moves the specimen at appropriate velocities for scanning. The error resulting from any flare of light emerging from outside of the area to be measured is minimized by limiting the illuminated area. The specimen can be observed during the measurement of light intensity by illuminating the whole microscope field at a wavelength different from that of the light used for the measurement. The retardation of the specimen is determined by comparing the specimen and background intensities as functions of the azimuth of a Brace-Köherl compensator. Alternatively, retardation is obtained directly from the light intensity at a fixed compensator angle, using the theory of polarization optics. The basal noise level for the present apparatus is approximately 0.03 nm when measuring birefringence of a 4-micron2 area in 0.1 s, using a X 40, NA 0.65 objective. The noise decreases in inverse proportion to the square root of the area times the duration of measurement.

2018 ◽  
Vol 2 (2) ◽  
pp. 95-99
Author(s):  
Sumarna Sumarna ◽  
Dyah Kurniawati Agustikasa ◽  
Agus Purwanto ◽  
Nur Kadarisman ◽  
Anggiyani Ratnaningtyas Eka Nugraheni ◽  
...  

Beberapa daerah di Wonogiri memiliki tanah yang mengandung kapur, sementara itu sebagian masyarakat mengonsumsi air yang berasal dari dalam tanah. Pada kegiatan pengabdian kepada masyarakat tahun 2016 ditemukan permasalahan dalam masyarakat di kelurahan Wonogiri yaitu sulitnya mendeteksi adanya kapur dalam air yang mereka konsumsi. Sebagai akibatnya banyak masyarakat yang menderita penyakit dalam yang diakibatkan pengendapan kapur di dalam tubuh mereka seperti penyakit ginjal. Oleh karenanya, tim PPM Fakultas MIPA Universitas Negeri Yogyakarta membangun sistem pendeteksi adanya kapur dalam cairan berbasis Light Emitting Diode (LED) dan Light Dependent Resistor (LDR). LED digunakan untuk menyinari cairan dengan kadar kapur yang berbeda-beda, kemudian LDR akan mendeteksi cahaya LED yang melewati cairan tersebut. Besar kecilnya nilai luaran LDR akan bergantung kadar kapur dalam cairan. Masyarakat sangat antusias dan mulai menggunakan alat ini untuk mendeteksi adanya kapur di air yang mereka konsumsi. Kata kunci: Kesadahan, LDR, LED Workshop On Detector of Water Hardness Based On Light Dependet Resistor In Giritontro Village, Giritontro District, Wonogiri RegencyAbstract           Some areas in Wonogiri contain hardness in its soil, while some people consume water that come from the soil. In community service activity in 2016, we found problems in the community in Giritontro, Wonogiri that is the difficulties to detect the hardness in the water that people consume. As a result, many people who suffer from internal diseases caused by the deposition of lime in their bodies such as kidney disease. Therefore, the PPM team of the Faculty of Mathematics and Natural Sciences of Yogyakarta State University built a hardness detection system in liquid based Light Emitting Diode (LED) and Light Dependent Resistor (LDR). LEDs are used to irradiate fluids with different lime levels, then LDR will detect the LED light passing through the liquid. The size of the LDR output value will depend on the lime content in the liquid. People are very enthusiastic and start using this tool to detect the presence of limestone in the water they consume. Keywords: Hardness, LDR, LED


Weed Science ◽  
1968 ◽  
Vol 16 (3) ◽  
pp. 329-331 ◽  
Author(s):  
P. J. Davies ◽  
D. E. Seaman

The production of red triphenyl formazan (hereinafter referred to as TPF) from colorless 2,3,5-triphenyl tetrazolium chloride (hereinafter referred to as TTC) by living cells was used as a vitality indicator for examining the effects of 6,7-dihydrodipyrido[1,2-a:2′,1′-c] pyrazidiinium salt (diquat) in elodea (Elodea canadensisMichx) shoots at varying light intensities. Diquat at 10 ppmw completely killed the elodea in 6 hr at 183 ft-c and at almost the same rate at 13,000 ft-c due to light saturation near the lower level. Over 24 hr were required for this effect in the dark. Between 29 and 183 ft-c, TPF production varied inversely with the light intensity. A comparison is made with the destruction of chlorophyll by diquat.


1975 ◽  
Vol 2 (3) ◽  
pp. 425 ◽  
Author(s):  
L Rahman ◽  
D Aspinall ◽  
LG Paleg

The influences of light intensity, rooting medium, and the excision of various organs on the early phases of root growth in barley seedlings (cv. Piroline) were explored. The emergence of root axes was affected by light intensity and excision of roots, endosperm or shoot, but was not altered by the rooring medium. Elongation of the emerged axes was similarly responsive. The elongation rate of both the set of axes and individual axes fell wi4h time. The elongation rate of laterals was less than that of the axes and was sensitive to the excision of seedling organs. The data are discussed in relation to the hypothesis that the growth of the root system is determined by the availability of substrate.


2008 ◽  
Vol 376 (1) ◽  
pp. 134-138 ◽  
Author(s):  
Younggiu Lee ◽  
Tadao Asami ◽  
Isomaro Yamaguchi ◽  
Hiroshi Ueda ◽  
Yoshihito Suzuki

2008 ◽  
Author(s):  
Jianshu Gao ◽  
Jiye Song ◽  
Zhijing Yu ◽  
Fei Chen ◽  
Xudong Shi ◽  
...  

2020 ◽  
Vol 19 (01) ◽  
pp. 11-22
Author(s):  
Almira Budiyanto ◽  
Abdul Manan ◽  
Elvira Sukma Wahyuni

The more advanced the technology and the greater the community's need to carry out activities every day, the number of vehicles on the highway is getting crowded. From year to year, the greater the level of traffic accidents caused by many factors, among the usual reasons is the loss of awareness of the driver when driving a vehicle especially drowsiness. One of the drowsiness parameters is the frequency eye blinks. Therefore, to get the drowsiness symptoms, the purpose of this research is to detect the eye blinks, which in turn reduce the level of accidents by detecting sleepy eyes based on digital image processing. The method used to detect both eyes is the Viola-Jones method. The detection of both eyes can also acquire the duration of closed eyes and the number of eye blinks. A person can be said to be sleepy by means of sleepiness parameters determined by a study. The research shows that detection of eye blinks using the Viola-Jones method has a fairly high accuracy of up to 84.72% if the face condition is upright and tilted no more than 45 degrees. Another conclusion is that eye detection and driver detection are more effective at certain light intensity values which are around 2-33 lux.


Electronics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 89 ◽  
Author(s):  
Christopher Goodin ◽  
Daniel Carruth ◽  
Matthew Doude ◽  
Christopher Hudson

While it is well known that rain may influence the performance of automotive LIDAR sensors commonly used in ADAS applications, there is a lack of quantitative analysis of this effect. In particular, there is very little published work on physically-based simulation of the influence of rain on terrestrial LIDAR performance. Additionally, there have been few quantitative studies on how rain-rate influences ADAS performance. In this work, we develop a mathematical model for the performance degradation of LIDAR as a function of rain-rate and incorporate this model into a simulation of an obstacle-detection system to show how it can be used to quantitatively predict the influence of rain on ADAS that use LIDAR.


1965 ◽  
Vol 20 (7) ◽  
pp. 699-701 ◽  
Author(s):  
Hedwig Hilgenheger ◽  
Wilhelm Menke

Like isolated chloroplasts, the volume of chloroplasts in living cells of Nitella flexilis decreases reversibly with illumination. This process was recorded by flashlight photography in the same cell and with the same chloroplast. These changes of volume are anisotropic and are caused by a decrease of the chloroplast thickness. The degree of shrinkage depends upon the age of the cells. A dependence of the light intensity was demonstrated. The correlation between fine structure of Nitella chloroplasts and of swelling and shrinkage phenomenon is discussed.


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