scholarly journals Widespread distribution of a 210,000 mol wt microtubule-associated protein in cells and tissues of primates.

1980 ◽  
Vol 87 (3) ◽  
pp. 802-808 ◽  
Author(s):  
J C Bulinski ◽  
G G Borisy
Keyword(s):  
Human Cell Line ◽  
Immunofluorescent Staining ◽  
Reactive Material ◽  
Widespread Distribution ◽  
Cell Line Hela ◽  
Indirect Immunofluorescence Technique ◽  
Related Proteins ◽  
Indirect Immunofluorescent ◽  
Line Hela ◽  
In Cells

Antisera prepared against a 210,000 mol wt microtubule-associated protein (210k MAP) isolated from the human cell line, HeLa, were used to survey a variety of cells and tissues for the presence of immunologically related proteins. The antisera were employed to test extracts of the cells and tissues, using a sensitive indirect immunofluorescence technique applied to polyacrylamide gels. Cross-reactive material of 210,000 mol wt was found in 10 kinds of cells and tissues derived from humans and four lines of cells from monkeys. Indirect immunofluorescent staining was also carried out on fixed cells and showed that the cross-reactive material was localized to interphase and mitotic microtubules as assayed in nine human and seven monkey cell lines. No protein that cross-reacted with 210k MAP antisera was detected in cells and tissues derived from two rodents, an ungulate, a marsupial, or a chicken. Therefore, the 210k MAP isolated from HeLa cells is present in a wide variety of cells and tissues of humans and other primates but is antigenically distinct from MAPs present in lower organisms.

scholarly journals miRNA Combinatorics and its Role in Cell State Control—A Probabilistic Approach

2021 ◽  
Vol 8 ◽  
Author(s):  
Shelly Mahlab-Aviv ◽  
Nathan Linial ◽  
Michal Linial
Keyword(s):  
Cell Lines ◽  
Probabilistic Approach ◽  
Human Cell Line ◽  
State Control ◽  
Cancer Evolution ◽  
Mirna Regulation ◽  
Cell Identity ◽  
Cell Line Hela ◽  
Stochastic Events ◽  
Line Hela

A hallmark of cancer evolution is that the tumor may change its cell identity and improve its survival and fitness. Drastic change in microRNA (miRNA) composition and quantities accompany such dynamic processes. Cancer samples are composed of cells’ mixtures of varying stages of cancerous progress. Therefore, cell-specific molecular profiling represents cellular averaging. In this study, we consider the degree to which altering miRNAs composition shifts cell behavior. We used COMICS, an iterative framework that simulates the stochastic events of miRNA-mRNA pairing, using a probabilistic approach. COMICS simulates the likelihood that cells change their transcriptome following many iterations (100 k). Results of COMICS from the human cell line (HeLa) confirmed that most genes are resistant to miRNA regulation. However, COMICS results suggest that the composition of the abundant miRNAs dictates the nature of the cells (across three cell lines) regardless of its actual mRNA steady-state. In silico perturbations of cell lines (i.e., by overexpressing miRNAs) allowed to classify genes according to their sensitivity and resilience to any combination of miRNA perturbations. Our results expose an overlooked quantitative dimension for a set of genes and miRNA regulation in living cells. The immediate implication is that even relatively modest overexpression of specific miRNAs may shift cell identity and impact cancer evolution.

Evaluating the cytotoxicity of ionic liquids using human cell line HeLa

2004 ◽  
Vol 23 (11) ◽  
pp. 513-517 ◽  
Author(s):  
P Stepnowski ◽  
A C Skladanowski ◽  
A Ludwiczak ◽  
E Laczyńska
Keyword(s):  
Ionic Liquids ◽  
Cell Line ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Human Cell Line ◽  
Imidazolium Ionic Liquids ◽  
Cell Line Hela ◽  
Order Of Magnitude ◽  
Line Hela

This paper presents cytotoxicity data of selected imidazolium ionic liquids evaluated in vitro on the human tumor cell line HeLa. It was found that for 1-n-butyl-3-methylimidazolium entities the toxicity depends strongly on the associated anion; EC50 values are lowest for tetrafluoroborate. No direct dependence of the reduced effect concentration was found on elongating the short, methyl chain to ethyl or n-hexyl. Only for the ionic liquid with an n-decyl chain, the longest one studied, did higher hydrophobicity result in a EC50 one order of magnitude lower than that obtained with the n-butyl entity. The effect concentrations of imidazolium ionic liquids in the HeLa system used are lower than the values obtained for conventional organic solvents such as dichloromethane, toluene or xylene.

Cytotoxicity of ionic liquids and precursor compounds towards human cell line HeLa

2007 ◽  
Vol 9 (11) ◽  
pp. 1191 ◽  
Author(s):  
Xuefeng Wang ◽  
C. André Ohlin ◽  
Qinghua Lu ◽  
Zhaofu Fei ◽  
Jun Hu ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Cell Line Hela ◽  
Line Hela

Henrietta Lacks, HeLa Cells, and Cell Culture Contamination

2009 ◽  
Vol 133 (9) ◽  
pp. 1463-1467 ◽  
Author(s):  
Brendan P. Lucey ◽  
Walter A. Nelson-Rees ◽  
Grover M. Hutchins
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cell Line ◽  
Hela Cells ◽  
Human Cell Line ◽  
Hela Cell Line ◽  
Cell Line Hela ◽  
The World ◽  
Cell Culture Contamination ◽  
Line Hela

Abstract Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer cells, now called HeLa cells, grew rapidly in cell culture and became the first human cell line. HeLa cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa cells contaminated and overgrew other cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa cells. We describe the history behind the development of HeLa cells, including the first published description of Ms Lacks' autopsy, and the cell culture contamination that resulted. The debate over cell culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of the immortal HeLa cell line.

scholarly journals Immunohistochemical localization of rK9, an enzyme of the kallikrein gene family, in the rat ventral prostate.

1995 ◽  
Vol 43 (1) ◽  
pp. 61-65 ◽  
Author(s):  
T Berg ◽  
H Schøyen
Keyword(s):  
Immunohistochemical Localization ◽  
Immunofluorescent Staining ◽  
Ventral Prostate ◽  
Staining Reaction ◽  
Specific Staining ◽  
Rat Ventral Prostate ◽  
Indirect Immunofluorescence Technique ◽  
Rat Prostate ◽  
Related Proteins ◽  
Rat Submandibular Gland

The rat kallikrein family consists of multiple closely related proteins. Most of these proteins have been purified from rat submandibular gland (SMG), among them rK9, an enzyme that has a direct vasoconstrictory effect on isolated aortic rings. Recently, two members of the kallikrein family, rK8 and rK9, were also purified from rat prostate, whereas other family members were not detected in this organ. In the present study, the immunohistochemical localization of rK9 was determined in rat prostate by the indirect immunofluorescence technique with polyclonal antisera against rat SMG rK9 in the first layer. The crossreactivity pattern of the antiserum against rat prostate tissue extract was established by flat-bed isoelectrofocusing and immunoblotting. The antiserum reacted with prostate rK9 but not with prostate rK8. Specificity of the immunofluorescent staining reaction was verified by staining with the primary antiserum after addition of purified SMG or prostate rK9, or other members of the kallikrein family including prostate rK8. rK9-specific immunofluorescence was detected in the prostate ductal structures. In the distal (acinar) segments, rK9 was found in the cytoplasm as granular staining in the middle third of the cell, in the luminal cell wall, and in the secretion within the lumen. In the intermediate segments, strong rK9-specific staining was observed adhering to the luminal wall as well as abundantly within the ductal lumen.

High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

1992 ◽  
Vol 68 (02) ◽  
pp. 119-124 ◽  
Author(s):  
F G Falkner ◽  
P L Turecek ◽  
R T A MacGillivray ◽  
W Bodemer ◽  
F Scheiflinger ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Cell Lines ◽  
Expression System ◽  
Human Cell Line ◽  
Cell System ◽  
Time Saving ◽  
Expression Levels ◽  
Mammalian Cell Lines ◽  
Cell Line Hela ◽  
Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

Photodynamic therapy in a cervico-uterine cancer cell line (HeLa) implanted in nu/nu mice

2001 ◽  
Author(s):  
Eva Ramon Gallegos ◽  
Salomon Hernandez Guitierrez ◽  
Leticia Garduno Siciliano ◽  
Jose L. Jiminez Perez ◽  
Aura J. Perez Zapata ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Line ◽  
Cancer Cell ◽  
Uterine Cancer ◽  
Cancer Cell Line ◽  
Cell Line Hela ◽  
Line Hela
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