Cytotoxicity of ionic liquids and precursor compounds towards human cell line HeLa

2007 ◽  
Vol 9 (11) ◽  
pp. 1191 ◽  
Author(s):  
Xuefeng Wang ◽  
C. André Ohlin ◽  
Qinghua Lu ◽  
Zhaofu Fei ◽  
Jun Hu ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Cell Line Hela ◽  
Line Hela

Evaluating the cytotoxicity of ionic liquids using human cell line HeLa

2004 ◽  
Vol 23 (11) ◽  
pp. 513-517 ◽  
Author(s):  
P Stepnowski ◽  
A C Skladanowski ◽  
A Ludwiczak ◽  
E Laczyńska
Keyword(s):  
Ionic Liquids ◽  
Cell Line ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Human Cell Line ◽  
Imidazolium Ionic Liquids ◽  
Cell Line Hela ◽  
Order Of Magnitude ◽  
Line Hela

This paper presents cytotoxicity data of selected imidazolium ionic liquids evaluated in vitro on the human tumor cell line HeLa. It was found that for 1-n-butyl-3-methylimidazolium entities the toxicity depends strongly on the associated anion; EC50 values are lowest for tetrafluoroborate. No direct dependence of the reduced effect concentration was found on elongating the short, methyl chain to ethyl or n-hexyl. Only for the ionic liquid with an n-decyl chain, the longest one studied, did higher hydrophobicity result in a EC50 one order of magnitude lower than that obtained with the n-butyl entity. The effect concentrations of imidazolium ionic liquids in the HeLa system used are lower than the values obtained for conventional organic solvents such as dichloromethane, toluene or xylene.

scholarly journals Cytotoxic Effects of Imidazolium Ionic Liquids on Fish and Human Cell Lines

2012 ◽  
Vol 63 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Marina Cvjetko ◽  
Kristina Radošević ◽  
Andrej Tomica ◽  
Igor Slivac ◽  
Jasna Vorkapić-Furač ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Cell Line ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Cytotoxic Effects ◽  
Imidazolium Ionic Liquids ◽  
Cell Line Hela ◽  
Wide Range ◽  
The Impact

Cytotoxic Effects of Imidazolium Ionic Liquids on Fish and Human Cell LinesIonic liquids bring a promise of a wide range of "green" applications that could replace conventional volatile solvents. However, before these applications become large-scale, their toxicity needs to be investigated in order to predict the impact on human health and environment. In this study we assessed the cytotoxicity of imidazolium ionic liquids (in the concentrations between 0.1 mmol L-1 and 10 mmol L-1) in the ovarian fish cell line CCO and the human tumour cell line HeLa using the MTT cell viability assay. Our results showed that the most cytotoxic ionic liquid was 1-n-butyl-3-methylimidazolium bis(trifluoro methylsulphonyl)imide, [BMIM][Tf2N], followed by 1-n-butyl-3-methylimidazolium tetrafluoroborate [BMIM][BF4], 1-n-butyl-3-methylimidazolium hexafluorophosphate [BMIM][PF6], and 1,3-dimethylimidazolium hexafluorophosphate [MMIM][PF6]. Generally, the effects were concentration-dependent. They also depended on the type of anion and the n-alkyl chain length. The comparison between the fish CCO and human HeLa cell lines suggests that CCO cells provide a good biological system for initial toxicity testing of ionic liquids that could replace in vivo bioassays.

Henrietta Lacks, HeLa Cells, and Cell Culture Contamination

2009 ◽  
Vol 133 (9) ◽  
pp. 1463-1467 ◽  
Author(s):  
Brendan P. Lucey ◽  
Walter A. Nelson-Rees ◽  
Grover M. Hutchins
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cell Line ◽  
Hela Cells ◽  
Human Cell Line ◽  
Hela Cell Line ◽  
Cell Line Hela ◽  
The World ◽  
Cell Culture Contamination ◽  
Line Hela

Abstract Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer cells, now called HeLa cells, grew rapidly in cell culture and became the first human cell line. HeLa cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa cells contaminated and overgrew other cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa cells. We describe the history behind the development of HeLa cells, including the first published description of Ms Lacks' autopsy, and the cell culture contamination that resulted. The debate over cell culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of the immortal HeLa cell line.

scholarly journals Estrogen Receptor in a Human Cell Line (MCF-7) from Breast Carcinoma

1973 ◽  
Vol 248 (17) ◽  
pp. 6251-6253 ◽  
Author(s):  
Samuel C. Brooks ◽  
Elizabeth R. Locke ◽  
Herbert D. Soule
Keyword(s):  
Estrogen Receptor ◽  
Breast Carcinoma ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Mcf 7

Local Regulation of Adrenal Steroidogenesis: Subtle in vitro Effects of IL-1β on the Human Cell Line NCI-H295R Steroid Production along with Changes in MicroRNA Profile and Orphan Nuclear Receptors NR4As

2021 ◽  
pp. 1-11
Author(s):  
Natalia Santucci ◽  
Rocío Stampone ◽  
Eduardo Brandão Ferreira da Silva ◽  
Silvina Villar ◽  
Silvana Spinelli ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Cell Line ◽  
Nuclear Receptors ◽  
Human Cell ◽  
Human Cell Line ◽  
Mirna Profile ◽  
Orphan Nuclear Receptors ◽  
Steroid Production ◽  
Adrenal Steroid ◽  
Adrenal Steroidogenesis

<b><i>Introduction:</i></b> IL-1β, a cytokine from the innate immune response, is well known for its proinflammatory effects and stimulating activity on the hypothalamus-pituitary-adrenal axis, leading to the pituitary synthesis of adrenocorticotropic hormone followed by cortisol (and dehydroepiandrosterone – DHEA) release by the adrenal gland. While IL-1β modulates the adrenal steroidogenesis at the central level, it is unclear whether it also exerts an effect on the adrenal gland. <b><i>Method:</i></b> We studied the effect of IL-1β on adrenal steroid production and steroidogenic enzyme RNA expression in the human cell line NCI-H295R. We also explored eventual changes in the microRNA (miRNA) profile from IL-1β-treated NCI-H295R cells. <b><i>Results:</i></b> Transcripts encoding IL-1β receptors 1 and 2 were noticeable in the cell line, with cortisol and DHEA production showing a subtle increase after cytokine treatment. Transcripts from key enzymes in the steroidogenic pathway were analyzed, with no noticeable changes on them. The miRNA profile was modified by IL-1β treatment to an extent which bears some relationship with the regulatory mechanisms underlying adrenal steroid production. Since orphan nuclear receptors NR4As have emerged as potential key factors for coordinating inflammatory and metabolic responses, cell expression studies were also carried out to show an NR4As transcript augmentation following IL-1β treatment. <b><i>Discussion/Conclusions:</i></b> The subtle increase in adrenal steroid production in response to IL-1β stimulation without any modification in the transcription of the steroidogenic enzymes analyzed suggests an additional inflammatory/anti-inflammatory loop, wherein NR4As receptors may participate. Besides its physiological role, this process might be implied in pathological states accompanied by an unbalanced immune-endocrine relationship.

Photodynamic therapy in a cervico-uterine cancer cell line (HeLa) implanted in nu/nu mice

2001 ◽  
Author(s):  
Eva Ramon Gallegos ◽  
Salomon Hernandez Guitierrez ◽  
Leticia Garduno Siciliano ◽  
Jose L. Jiminez Perez ◽  
Aura J. Perez Zapata ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cell Line ◽  
Cancer Cell ◽  
Uterine Cancer ◽  
Cancer Cell Line ◽  
Cell Line Hela ◽  
Line Hela

Reduced cytotoxicity of tetracyclines to a multi-drug resistant human cell line

1988 ◽  
Vol 37 (15) ◽  
pp. 3038-3041 ◽  
Author(s):  
Maria Grandi ◽  
Fernando C. Giuliani
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Drug Resistant
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