scholarly journals OBSERVATIONS ON THE UNUSUAL CYTOPLASMIC LACUNAE OF THE PORCINE HEPATOCYTE

1972 ◽  
Vol 54 (2) ◽  
pp. 434-438 ◽  
Author(s):  
Roger F. Searle
Keyword(s):  
ASAIO Journal ◽  
2001 ◽  
Vol 47 (2) ◽  
pp. 164
Author(s):  
A. Lorenti ◽  
M. Barbich ◽  
P. Sorroche ◽  
A. Schenone ◽  
A. Guinle ◽  
...  

1996 ◽  
Vol 19 (10) ◽  
pp. 605-609 ◽  
Author(s):  
K. Naruse ◽  
Y. Sakai ◽  
I. Nagashima ◽  
G.X. Jiang ◽  
M. Suzuki ◽  
...  

We previously developed a new bioreactor of the bioartificial liver composed of non-woven fabric. We have also experimented with hepatocyte spheroids, with the aim of improving the efficiency of this NWF bioreactor. In this study, we compared the efficiencies of NWF bioreactors employing hepatocyte spheroids versus single hepatocytes. Hepatocytes were isolated from a whole pig liver by Seglen's method. 1.0 × 1010 single hepatocytes were immobilized in the NWF bioreactor. Another 1.0 × 1010 hepatocytes were allowed to form spheroids by 24 hr suspension culture in a 4-L culture vessel, before being immobilized in the bioreactor. Hepatocyte spheroids were found to be functionally superior, on a per-cell basis, to single hepatocytes in the NWF bioreactor. However, the NWF bioreactor employing hepatocyte spheroids exhibited lower efficiency than that employing single hepatocytes, because the total number of the hepatocytes had decreased during the 24 hr suspension culture.


2014 ◽  
Vol 21 (5) ◽  
pp. 444-453 ◽  
Author(s):  
Wolf Ramackers ◽  
Johannes Klose ◽  
Florian W. R. Vondran ◽  
Harald Schrem ◽  
Alexander Kaltenborn ◽  
...  

2001 ◽  
Vol 10 (8) ◽  
pp. 731-738 ◽  
Author(s):  
Paul G. Gregory ◽  
Christopher K. Connolly ◽  
Bryan E. Gillis ◽  
Susan J. Sullivan

Toxicology ◽  
2004 ◽  
Vol 204 (2-3) ◽  
pp. 241-249 ◽  
Author(s):  
Osamu Mikami ◽  
Sachiko Yamamoto ◽  
Noriko Yamanaka ◽  
Yasuyuki Nakajima

2019 ◽  
Vol 26 (4) ◽  
Author(s):  
Yi Li ◽  
Harvey S. Chen ◽  
Mohammed Shaheen ◽  
Dong Jin Joo ◽  
Bruce P. Amiot ◽  
...  

2010 ◽  
Vol 192 (2) ◽  
pp. 125-140 ◽  
Author(s):  
Leonard J. Nelson ◽  
Simon W. Walker ◽  
Peter C. Hayes ◽  
John N. Plevris

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