scholarly journals Low-Shear Modelled Microgravity Environment Maintains Morphology and Differentiated Functionality of Primary Porcine Hepatocyte Cultures

2010 ◽  
Vol 192 (2) ◽  
pp. 125-140 ◽  
Author(s):  
Leonard J. Nelson ◽  
Simon W. Walker ◽  
Peter C. Hayes ◽  
John N. Plevris
Keyword(s):  
Microgravity Environment ◽  
Hepatocyte Cultures ◽  
Porcine Hepatocyte ◽  
Low Shear

Employment of a Bioreactor Vessel to Simulate Elements of Microgravity During Early Chick Heart Development

1997 ◽  
Vol 3 (S2) ◽  
pp. 171-172
Author(s):  
W.N. Norton ◽  
D. Wiens
Keyword(s):  
Heart Development ◽  
Microgravity Environment ◽  
Altered Gravity ◽  
Crucial Component ◽  
Production And Distribution ◽  
Chick Heart ◽  
Chicken Eggs ◽  
The Relationship ◽  
Migratory Process ◽  
Low Shear

Formation of the chick heart is initiated when paired regions of the mesoderm migrate to a specific region in the primitive head. Certain cells eventually form a pair of endocardiac tubes that become the lining of the heart, while the remaining precardiac mesoderm ultimately differentiates into the myocardium. Fibronectin is suspected as a crucial component of the migratory process. Very few investigations have been conducted on the relationship between cell motility and microgravity.The primary objectives of this investigation were to determine the effect of altered gravity on the production and distribution of fibronectin, an extracellular matrix glycoprotein, during embryonic development of the chick heart, and to ascertain the extent to which these alterations affect myogenesis or morphogenesis. A microgravity bioreactor vessel, originally designed by NASA as a low-shear culture system, was employed to culture the cellular expiants in a simulated microgravity environment. Fertile, White-Leghorn chicken eggs were incubated at 38°C for 32 hours in a humidified egg incubator.

scholarly journals Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment

2007 ◽  
Vol 7 (1) ◽  
pp. 15 ◽  
Author(s):  
Don L Tucker ◽  
C Ott ◽  
Stephen Huff ◽  
Yuriy Fofanov ◽  
Duane L Pierson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Microgravity Environment ◽  
Modeled Microgravity ◽  
Low Shear

Cryopreserved porcine hepatocyte cultures

1999 ◽  
Vol 121 (1) ◽  
pp. 99-115 ◽  
Author(s):  
H.G. Koebe ◽  
B. Mühling ◽  
C.J. Deglmann ◽  
F.W. Schildberg
Keyword(s):  
Hepatocyte Cultures ◽  
Porcine Hepatocyte

Cryopreservation of Porcine Hepatocyte Cultures

Cryobiology ◽  
1996 ◽  
Vol 33 (1) ◽  
pp. 127-141 ◽  
Author(s):  
H.G. KOEBE ◽  
C. DÄHNHARDT ◽  
J. MÜLLER-HÖCKER ◽  
H. WAGNER ◽  
F.W. SCHILDBERG
Keyword(s):  
Hepatocyte Cultures ◽  
Porcine Hepatocyte

scholarly journals Evaluation of Acquired Antibiotic Resistance inEscherichia coliExposed to Long-Term Low-Shear Modeled Microgravity and Background Antibiotic Exposure

mBio ◽  
2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Madhan R. Tirumalai ◽  
Fathi Karouia ◽  
Quyen Tran ◽  
Victor G. Stepanov ◽  
Rebekah J. Bruce ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Stress Factors ◽  
Microgravity Environment ◽  
Antibiotic Exposure ◽  
Modeled Microgravity ◽  
Term Response ◽  
Control Study ◽  
Acquired Antibiotic Resistance ◽  
Low Shear

ABSTRACTThe long-term response of microbial communities to the microgravity environment of space is not yet fully understood. Of special interest is the possibility that members of these communities may acquire antibiotic resistance. In this study,Escherichia colicells were grown under low-shear modeled microgravity (LSMMG) conditions for over 1,000 generations (1000G) using chloramphenicol treatment between cycles to prevent contamination. The results were compared with data from an earlier control study done under identical conditions using steam sterilization between cycles rather than chloramphenicol. The sensitivity of the final 1000G-adapted strain to a variety of antibiotics was determined using Vitek analysis. In addition to resistance to chloramphenicol, the adapted strain acquired resistance to cefalotin, cefuroxime, cefuroxime axetil, cefoxitin, and tetracycline. In fact, the resistance to chloramphenicol and cefalotin persisted for over 110 generations despite the removal of both LSMMG conditions and trace antibiotic exposure. Genome sequencing of the adapted strain revealed 22 major changes, including 3 transposon-mediated rearrangements (TMRs). Two TMRs disrupted coding genes (involved in bacterial adhesion), while the third resulted in the deletion of an entire segment (14,314 bp) of the genome, which includes 14 genes involved with motility and chemotaxis. These results are in stark contrast with data from our earlier control study in which cells grown under the identical conditions without antibiotic exposure never acquired antibiotic resistance. Overall, LSMMG does not appear to alter the antibiotic stress resistance seen in microbial ecosystems not exposed to microgravity.IMPORTANCEStress factors experienced during space include microgravity, sleep deprivation, radiation, isolation, and microbial contamination, all of which can promote immune suppression (1, 2). Under these conditions, the risk of infection from opportunistic pathogens increases significantly, particularly during long-term missions (3). If infection occurs, it is important that the infectious agent should not be antibiotic resistant. Minimizing the occurrence of antibiotic resistance is, therefore, highly desirable. To facilitate this, it is important to better understand the long-term response of bacteria to the microgravity environment. This study demonstrated that the use of antibiotics as a preventive measure could be counterproductive and would likely result in persistent resistance to that antibiotic. In addition, unintended resistance to other antimicrobials might also occur as well as permanent genome changes that might have other unanticipated and undesirable consequences.

Toxicological properties of thymoquinone in primary rat hepatocyte cultures

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
M Khader ◽  
N Bresgen ◽  
P Eckl
Keyword(s):  
Rat Hepatocyte ◽  
Hepatocyte Cultures

Bubble motion in liquid nitrogen under a non-uniform electric field in a microgravity environment

1997 ◽  
Vol 117 (11) ◽  
pp. 1109-1114
Author(s):  
Yoshiyuki Suda ◽  
Kenji Mutoh ◽  
Yosuke Sakai ◽  
Kiyotaka Matsuura ◽  
Norio Homma
Keyword(s):  
Electric Field ◽  
Liquid Nitrogen ◽  
Microgravity Environment ◽  
Uniform Electric Field ◽  
Bubble Motion
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