scholarly journals FINE STRUCTURE OF THE MIDDLE LAMELLA OF AGGREGATES OF PLANT CELLS IN SUSPENSION CULTURE

1971 ◽  
Vol 50 (1) ◽  
pp. 237-246 ◽  
Author(s):  
Gary G. Leppard ◽  
J. Ross Colvin
Keyword(s):  
Fine Structure ◽  
Suspension Culture ◽  
Plant Cells ◽  
Middle Lamella

Accumulation of phytoalexins in the compatible interaction between Cladosporium fulvum and tomato in relation to colonization

1991 ◽  
Vol 69 (4) ◽  
pp. 822-830 ◽  
Author(s):  
Ulisses G. Batista ◽  
Verna J. Higgins
Keyword(s):  
Suspension Culture ◽  
Germ Tube ◽  
Plant Cells ◽  
Cladosporium Fulvum ◽  
In Vitro Experiments ◽  
Compatible Interaction ◽  
Leaf Mold ◽  
Production And Distribution ◽  
Fulvia Fulva

The production and distribution of the phytoalexin falcarindiol in tomato foliage infected with leaf mold was examined to determine how the fungus Cladosporium fulvum is able to colonize and sporulate in an apparently antifungal environment. In a compatible interaction (cv. Potentate – C. fulvum race 2.3), by 12 and 15 days after inoculation, solvent-extractable falcarindiol and two other phytoalexins from tomato, compound 2 (probably falcarinol) and compound 3 (unidentified), reached concentrations considerably in excess of ED50 values for inhibition of the fungus. In contrast, intercellular (apoplastic) fluids obtained from similarly infected leaflets contained only traces of falcarindiol. ED50 values for germination and germ-tube growth of C. fulvum increased as the incubation time was extended, suggesting that adaptation or recovery was possible at the concentrations tested. In in vitro experiments, C. fulvum appeared to readily metabolize falcarindiol, as did a Lycopersicon cell suspension culture. Binding of falcarindiol to living and dead fungal and plant cells was also observed. Falcarindiol, injected into tomato leaflets, decreased rapidly and was only recovered in trace amounts by 24 h. The results suggest that falcarindiol and probably the two other phytoalexins do not reach sufficient concentrations in the apoplast of an infected susceptible leaf to have an effect on growth and sporulation of C. fulvum. Key words: leaf mold, Fulvia fulva, falcarindiol, falcarinol.

Observations with cytochemistry and ultracryotomy on the fine structure of the expanding walls in actively elongating plant cells

1975 ◽  
Vol 19 (2) ◽  
pp. 239-259
Author(s):  
J.C. Roland ◽  
B. Vian ◽  
D. Reis
Keyword(s):  
Fine Structure ◽  
Periodic Acid ◽  
Middle Lamella ◽  
Positive Control ◽  
Apium Graveolens ◽  
Wall Structure ◽  
Primary Wall ◽  
Environmental Signals ◽  
3 Dimensional ◽  
Complementary Techniques

Ultracryotomy with negative staining and cytochemistry (periodic acid - thiocarbohydrazide - silver proteinate test for polysaccharides, in conjunction with mild extractions) were used to study the architecture of the cell wall and its modifications during expansion. Those techniques were applied to the study in situ of the walls of actively elongating parenchyma of mung bean (Phaseolus aureus), and pea (Pisum sativum) root and of collenchyma of celery (Apium graveolens) petioles. These complementary techniques provide information on the 3-dimensional disposition and fine structure of the subunits of the wall. In all the examples examined, the bulk of growing primary wall appears well-ordered and no progressive evolution from a transverse texture near the plasmalemma to a scattered texture near the middle lamella was observed. It seems unlikely that the development of the wall structure in relation to growth could be explained mechanically by a passive shift of the fibrillar elements in response to cellular stress. There is no evidence for an inert change in fibrillar orientation in the major part of the wall. If such occurs the process is limited to the outermost and senescent part of the wall. Thus, the texture observed does not agree with the classical multinet growth hypothesis but rather with the idea of an ordered structure of the primary wall. With the latter, the components should be able to respond in different ways to specific growth regulators and other environmental signals and thus exert a more positive control over the processes of oriented cell growth.

Introduction to the Fine Structure of Plant Cells.

1972 ◽  
Vol 99 (1) ◽  
pp. 40
Author(s):  
J. F. Gennaro ◽  
Myron C. Ledbetter ◽  
Keith R. Porter
Keyword(s):  
Fine Structure ◽  
Plant Cells

Introduction to the fine structure of plant cells

1971 ◽  
Vol 10 (11) ◽  
pp. 2856-2857
Author(s):  
D.M. Keith-Lucas
Keyword(s):  
Fine Structure ◽  
Plant Cells
Sign in / Sign up

Export Citation Format

Share Document