Studies on the Growth in Culture of Plant Cells: II. CHANGES IN RESPIRATION RATE AND NITROGEN CONTENT ASSOCIATED WITH THE GROWTH OFACER PSEUDOPLATANUSL. CELLS IN SUSPENSION CULTURE

1967 ◽  
Vol 18 (2) ◽  
pp. 321-331 ◽  
Author(s):  
CURTIS V. GIVAN ◽  
H. A. COLLIN
Keyword(s):  
Suspension Culture ◽  
Nitrogen Content ◽  
Respiration Rate ◽  
Plant Cells

Accumulation of phytoalexins in the compatible interaction between Cladosporium fulvum and tomato in relation to colonization

1991 ◽  
Vol 69 (4) ◽  
pp. 822-830 ◽  
Author(s):  
Ulisses G. Batista ◽  
Verna J. Higgins
Keyword(s):  
Suspension Culture ◽  
Germ Tube ◽  
Plant Cells ◽  
Cladosporium Fulvum ◽  
In Vitro Experiments ◽  
Compatible Interaction ◽  
Leaf Mold ◽  
Production And Distribution ◽  
Fulvia Fulva

The production and distribution of the phytoalexin falcarindiol in tomato foliage infected with leaf mold was examined to determine how the fungus Cladosporium fulvum is able to colonize and sporulate in an apparently antifungal environment. In a compatible interaction (cv. Potentate – C. fulvum race 2.3), by 12 and 15 days after inoculation, solvent-extractable falcarindiol and two other phytoalexins from tomato, compound 2 (probably falcarinol) and compound 3 (unidentified), reached concentrations considerably in excess of ED50 values for inhibition of the fungus. In contrast, intercellular (apoplastic) fluids obtained from similarly infected leaflets contained only traces of falcarindiol. ED50 values for germination and germ-tube growth of C. fulvum increased as the incubation time was extended, suggesting that adaptation or recovery was possible at the concentrations tested. In in vitro experiments, C. fulvum appeared to readily metabolize falcarindiol, as did a Lycopersicon cell suspension culture. Binding of falcarindiol to living and dead fungal and plant cells was also observed. Falcarindiol, injected into tomato leaflets, decreased rapidly and was only recovered in trace amounts by 24 h. The results suggest that falcarindiol and probably the two other phytoalexins do not reach sufficient concentrations in the apoplast of an infected susceptible leaf to have an effect on growth and sporulation of C. fulvum. Key words: leaf mold, Fulvia fulva, falcarindiol, falcarinol.

Influence of fertilizer treatment on apple fruit composition and physiology. II. Influence on respiration rate and contents of nitrogen, phosphorus, and titratable acidity

1969 ◽  
Vol 20 (6) ◽  
pp. 1073 ◽  
Author(s):  
DS Letham
Keyword(s):  
Nitrogen Content ◽  
Respiration Rate ◽  
Phosphorus Content ◽  
Titratable Acidity ◽  
Dry Weight ◽  
Apple Fruit ◽  
Fertilizer Treatment ◽  
Soluble Nitrogen ◽  
Tissue Contents ◽  
Nitrogen Phosphorus

The respiration rates and tissue contents of nitrogen, phosphorus, and titratable acidity were determined for apple fruit from trees receiving differential fertilizer treatments U, N, P, NP, and NPK (U, no fertilizer; N, 2 lb ammonium sulphate; P, 4 1b superphosphate; and K, 1 Ib potassium sulphate, per tree per year). The results obtained (expressed on a dry weight basis) are summarized as follows: Respiration rate: N > NP = NPK = U > P. Total nitrogen content: NPK > NP = N > U = P. Ethanol-insoluble nitrogen content: NPK = NP = N > U = P. Ethanol-soluble nitrogen content: NPK > NP = N > U = P. Phosphorus content : P > NP = NPK > U > N. Titratable acidity content: NPK > N > NP = U > P. The incidence of internal breakdown in stored fruit from these treatments has positively correlated with respiration rate per cell and negatively correlated with phosphorus content. Phosphorus content was negatively correlated with cell volume and respiration rate. Mechanisms by which phosphorus content might regulate the incidence of internal breakdown are discussed.

Formation of ubiquinone by tobacco plant cells in suspension culture

1976 ◽  
Vol 15 (4) ◽  
pp. 568-569 ◽  
Author(s):  
Tsutomu Ikeda ◽  
Takashi Matsumoto ◽  
Masao Noguchi
Keyword(s):  
Suspension Culture ◽  
Tobacco Plant ◽  
Plant Cells

Growth of suspension cultures of plant cells (Ipomoea sp.) at various temperatures

1975 ◽  
Vol 53 (4) ◽  
pp. 315-320 ◽  
Author(s):  
Dyson Rose ◽  
S. M. Martin
Keyword(s):  
Growth Rate ◽  
Suspension Culture ◽  
Cell Line ◽  
Plant Cells ◽  
Maximum Growth ◽  
Suspension Cultures ◽  
Root Explants ◽  
Temperature Variations ◽  
Satisfactory Method ◽  
Dry Cell

Studies on the growth of batch suspension cultures of a plant cell line, initiated from Ipomoea sp. root explants, at temperatures ranging from 15 to 34 °C are reported. Maximum growth of cultures of this cell line occurred between 25 and 32 °C, with temperature variations within this range having little effect on growth rates based on dry cell yields. Cultivation at 20 °C substantially reduced the growth rate. However, cells grown at 20 °C failed to grow on transfer to fresh medium at 20 °C. Thus cultivation at suboptimum temperatures is not a satisfactory method for routine maintenance of the suspension culture.

Sign in / Sign up

Export Citation Format

Share Document