scholarly journals MEMBRANE-BOUND RIBOSOMES IN KIDNEY

1969 ◽  
Vol 41 (3) ◽  
pp. 886-893 ◽  
Author(s):  
G. C. Priestley ◽  
R. A. Malt
Keyword(s):  
Optical Density ◽  
Orotic Acid ◽  
Normal Mouse ◽  
Compensatory Hypertrophy ◽  
Renal Growth ◽  
Compensatory Renal Growth ◽  
Secrete Protein ◽  
Membrane Bound ◽  
Post Mitochondrial Supernatant

Membrane-bound ribosomes are thought to secrete protein for export and free ribosomes to secrete protein for intracellular use. The proportion of the total ribosomes that is bound to membranes in normal mouse kidneys has been estimated by three different methods, and the results have been compared with those obtained by a fourth method used by us previously. The most valid estimates appear to be those obtained (a) by comparison of radioactivity in peaks representing free and membrane-bound ribosomes on linear sucrose gradients after labeling for 24 hr with 14C-orotic acid, and (b) by measurements of optical density in free and bound ribosomes that had been separated by centrifugation on discontinuous gradients of 0.5 M/2.0 M sucrose. Analyses by these methods show that about 20–25% of the ribosomes in a postnuclear supernatant prepared from mouse kidneys, but only 10–15% of the ribosomes in a post-mitochondrial supernatant, are membrane-bound. About 75% of the bound ribosomes sediment as polysomes of many different sizes. The proportion of membrane-bound ribosomes and their aggregation into polysomes were unchanged in kidneys undergoing compensatory hypertrophy after removal of the opposite kidney. These experiments show that, unlike liver, kidney has a predominance of free ribosomes compared to bound ribosomes; those ribosomes that are membrane-bound do not become free during compensatory renal growth.

CT Evaluation of Compensatory Renal Growth in Relation to Postnephrectomy Time

1992 ◽  
Vol 33 (6) ◽  
pp. 566-568 ◽  
Author(s):  
P. Prassopoulos ◽  
N. Gourtsoyiannis ◽  
D. Cavouras ◽  
N. Pantelidis
Keyword(s):  
Renal Carcinoma ◽  
Compensatory Hypertrophy ◽  
Renal Parenchyma ◽  
Renal Growth ◽  
Compensatory Renal Growth ◽  
Kidney Size ◽  
Before And After ◽  
Ct Evaluation ◽  
Multiple Measurements ◽  
Parenchymal Thickness

In 27 patients nephrectoraized for renal carcinoma, the compensatory hypertrophy of the remaining kidney was assessed by 72 CT examinations performed one month before and during 32 months after nephrectomy. Kidney size was estimated on CT by multiple measurements of the renal parenchymal thickness. Kidney growth was evaluated by comparing the amount of renal parenchyma before and after contralateral nephrectomy. Renal compensatory hypertrophy varied with postnephrectomy time. Kidney enlargement was 15% in the first 3 months, reached maximum 30% about a year later, and was reduced to 5%, 2 1/2 years postoperatively.

Early increase in pulsatile growth hormone release after unilateral nephrectomy in adult rats

1994 ◽  
Vol 266 (4) ◽  
pp. F628-F632 ◽  
Author(s):  
A. Haramati ◽  
M. D. Lumpkin ◽  
S. E. Mulroney
Keyword(s):  
Growth Hormone ◽  
Early Time ◽  
Peak Level ◽  
Unilateral Nephrectomy ◽  
Sham Operation ◽  
Renal Growth ◽  
Adult Rats ◽  
Compensatory Renal Growth ◽  
Jugular Veins ◽  
Hypertrophic Response

Removal of one kidney results, within days, in accelerated growth of the remaining kidney. However, the mechanisms that underlie this compensatory renal hypertrophic response, particularly in the early time period following nephrectomy, are not understood. In this study we tested the hypothesis that removal of one kidney leads to a change in the pulsatile release of growth hormone (GH), which facilitates compensatory renal growth. Adult Wistar rats were implanted with Silastic cannulas in jugular veins and underwent either unilateral nephrectomy (UNX) or sham operation. Plasma levels of GH were determined 24 and 48 h after sham operation or UNX. Blood samples were taken every 20 min over a 6-h period from conscious, unrestrained animals. Pulsatile GH release was markedly elevated 24 h after UNX in both the amplitude of the surges as well as in the duration of release. Peak GH levels after 24 h were three- to fourfold higher in UNX rats compared with sham controls (417 +/- 75 vs. 119 +/- 23 ng/ml, P < 0.05). However, this enhanced release of GH appeared to be of short duration and began declining by 48 h post-UNX (peak level of 227 +/- 37 ng/ml, P < 0.05 vs. both 24 h UNX and sham controls). To examine whether this rise in GH release post-UNX contributed to the compensatory renal growth, rats underwent UNX and were immediately treated with an antagonist to GH-releasing factor (GRF-AN; i.e., [N-Ac-Tyr1,D-Arg2]GRF-(1-29) amide, 200 micrograms/kg twice daily), and the effects on GH release and renal growth were determined. Administration of GRF-AN significantly suppressed the increase in GH release post-UNX and was associated with a significant attenuation in renal growth 48 h post-UNX in GRF-AN-treated rats (8.7 +/- 2.6% vs. 22.7 +/- 3.0% in UNX controls, P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Age-dependent characteristics of compensatory renal growth

1975 ◽  
Vol 8 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Joel M. Kaufman ◽  
Robert Hardy ◽  
John P. Hayslett
Keyword(s):  
Renal Growth ◽  
Compensatory Renal Growth ◽  
Age Dependent

Effect of Treatment with Enalapril, Verapamil and Indomethacin on Compensatory Renal Growth in the Rat

1990 ◽  
Vol 5 (9) ◽  
pp. 777-780 ◽  
Author(s):  
J. P. Wight ◽  
A. H. Bassett ◽  
J. E. le Carpentier ◽  
A. M. El Nahas
Keyword(s):  
Renal Growth ◽  
Compensatory Renal Growth ◽  
Effect Of Treatment
Sign in / Sign up

Export Citation Format

Share Document