Shoot apical meristem: In vitro regeneration and morphogenesis in wheat (Triticum aestivum L.)

2002 ◽  
Vol 38 (2) ◽  
pp. 163-167 ◽  
Author(s):  
Anwaar Ahmad ◽  
Heng Zhong ◽  
Wengling Wang ◽  
Mariam B. Sticklen
Keyword(s):  
Triticum Aestivum ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Triticum Aestivum L

scholarly journals In vitro regeneration of pumpkin (cucurbita maxima) through shoot apical meristem

1970 ◽  
Vol 18 ◽  
pp. 104-107 ◽  
Author(s):  
ME Haque ◽  
D Rezwana ◽  
MA Islam ◽  
B Sikdar
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Total Yield ◽  
Meristem Culture ◽  
Culture Vessel ◽  
Cucurbita Maxima ◽  
Indirect Regeneration

Context: Pumpkin (Cucurbita maxima) is very important vegetable crop. It is damaged by several types of diseases, especially viral diseases which reduce the total yield of pumpkin. This is very difficult problem and such type of problem can be overcome by meristem culture. Objectives: To develop suitable protocols for indirect regeneration of pumpkin plant through shoot apical meristem.   Materials and Methods: Meristems were isolated from 15-21 days in vivo grown plants by collecting shoot tips and were prepared (0.2-0.5 mm) under 4X zoom stereo-microscope remaining two leaves primordia. Inoculation of explants was made singly per culture vessel in semisolid MS fortified with different concentrations and combinations of cytokinin and auxin.   Results: Among different concentrations, 1.0 mg/l BAP showed best response for callus induction. Calli were sub cultured for shoot formation in MS containing BAP singly or combination with GA3 and 1.5 mg/l BAP + 0.1 mg/l GA3 gave better result. In vitro regenerated shoots were rooted well in ½ strength MS with 0.5 mg/l IBA and micro plants were acclimatized successfully in natural condition.   Conclusion: Indirect regeneration of pumpkin plants through shoot apical meristem has been established.   Keywords: Growth regulators; in vitro regeneration; Pumpkin; Cucurbita maxima. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8784 JBS 2010; 18(0): 104-107

HERITABILITY OF IN VITRO REGENERATION IN WHEAT (Triticum aestivum L.)

1990 ◽  
Vol 70 (2) ◽  
pp. 547-550 ◽  
Author(s):  
N. CHEVRIER ◽  
J. A. QURESHI ◽  
K. K. KARTHA ◽  
P. HUCL
Keyword(s):  
Triticum Aestivum ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Triticum Aestivum L ◽  
Genotypic Differences ◽  
Additive Variance ◽  
Generation Means ◽  
Regeneration In Vitro ◽  
Embryogenic Callus Formation

This study measured the heritability of in vitro regeneration in wheat (Triticum aestivum L.). Immature embryos of 28 F2-derived families of the spring wheat cross HY912 × HY320 were cultured in the F3, F4, and F5 generations. Highly significant (P < 0.01) genotypic differences were observed for embryo germination, callus induction, embryogenic callus formation, and plantlet regeneration. Comparisons of generation means and mid-parent values suggest that additive variance is an important factor in the inheritance of in vitro regeneration.Key words: Regeneration, in vitro, heritability, Triticum aestivum, wheat

HAPLOID PLANTS (2n = 21) FROM in vitro ANTHER CULTURE OF TRITICUM AESTIVUM

1974 ◽  
Vol 16 (3) ◽  
pp. 697-700 ◽  
Author(s):  
Iris L. Craig
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Haploid Plants

Nine haploids (2n = 21) of Triticum aestivum L. (cv. Pitic–62) were obtained by in vitro anther culture, utilizing the medium described by Ouyang et al. (1973).

scholarly journals Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene

2018 ◽  
Vol 22 ◽  
pp. 222-227
Author(s):  
O. M. Honcharuk ◽  
O. V. Dubrovna
Keyword(s):  
Triticum Aestivum ◽  
Transgenic Plants ◽  
Bread Wheat ◽  
Binary Vector ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Pcr Analysis ◽  
Genetic Construct ◽  
Agrobacterium Mediated Transformation

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.

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