HERITABILITY OF IN VITRO REGENERATION IN WHEAT (Triticum aestivum L.)

1990 ◽  
Vol 70 (2) ◽  
pp. 547-550 ◽  
Author(s):  
N. CHEVRIER ◽  
J. A. QURESHI ◽  
K. K. KARTHA ◽  
P. HUCL
Keyword(s):  
Triticum Aestivum ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Triticum Aestivum L ◽  
Genotypic Differences ◽  
Additive Variance ◽  
Generation Means ◽  
Regeneration In Vitro ◽  
Embryogenic Callus Formation

This study measured the heritability of in vitro regeneration in wheat (Triticum aestivum L.). Immature embryos of 28 F2-derived families of the spring wheat cross HY912 × HY320 were cultured in the F3, F4, and F5 generations. Highly significant (P < 0.01) genotypic differences were observed for embryo germination, callus induction, embryogenic callus formation, and plantlet regeneration. Comparisons of generation means and mid-parent values suggest that additive variance is an important factor in the inheritance of in vitro regeneration.Key words: Regeneration, in vitro, heritability, Triticum aestivum, wheat

HAPLOID PLANTS (2n = 21) FROM in vitro ANTHER CULTURE OF TRITICUM AESTIVUM

1974 ◽  
Vol 16 (3) ◽  
pp. 697-700 ◽  
Author(s):  
Iris L. Craig
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Haploid Plants

Nine haploids (2n = 21) of Triticum aestivum L. (cv. Pitic–62) were obtained by in vitro anther culture, utilizing the medium described by Ouyang et al. (1973).

Selectivity of auxin for induction and growth of callus from excised embryo of spring and winter wheat

1984 ◽  
Vol 62 (7) ◽  
pp. 1393-1397 ◽  
Author(s):  
M. D. Zhou ◽  
T. T. Lee
Keyword(s):  
Winter Wheat ◽  
Chinese Spring ◽  
Shoot Growth ◽  
Callus Formation ◽  
Triticum Aestivum L ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Methoxybenzoic Acid

The callus-promoting activity of most commonly known as well as some rarely tested auxins was compared with that of 2,4-dichlorophenoxyacetic acid (2,4-D) for in vitro culture of the excised embryo of spring and winter wheat (Triticum aestivum L.), cv. Chinese Spring and cv. Fredrick. Different auxins in a concentration range from 1 to 50 μM showed widely different activities. Also the two wheat cultivars responded differently to the auxins. When rapid callus formation with limited root growth was used as the basis for comparison, 2-(2-methyl-4-chlorophenoxy)propionic acid (2-MCPP), α-naphthaleneacetic acid, 3,6-dichloro-2-methoxybenzoic acid (dicamba), 4-amino-3,5,6,trichloropicolinic acid (picloram), γ-(2,4-dichlorophenoxy)butyric acid, 2,4,5-trichlorophenoxyacetic acid, and 2,4,5-trichlorophenoxypropionic acid, in the order of effectiveness, were superior to 2,4,-D for callus induction from the embryo of 'Chinese Spring,' although the concentration required was higher than that of 2,4-D. For the winter wheat 'Fredrick,' however, only picloram, dicamba, and 2-MCPP performed as well as 2,4-D. All auxins tested promoted shoot growth; 2-methyl-4-chlorophenoxypropionic acid was most effective for 'Chinese Spring,' whereas picloram was most effective for 'Fredrick.'

scholarly journals CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

2015 ◽  
Vol 39 (3) ◽  
pp. 439-445 ◽  
Author(s):  
Laureen Michelle Houllou ◽  
Robson Antônio de Souza ◽  
Elizabete Cristina Pacheco dos Santos ◽  
José Jackson Pereira da Silva ◽  
Marta Ribeiro Barbosa ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Regenerative Process ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Stem Base ◽  
Different Characteristics

ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.

scholarly journals Proliferasi Kalus dari Eksplan Hipokotil dan Kotiledon Tanaman Jarak Pagar (Jatropha curcas L.) pada Pemberian 2,4-D

2012 ◽  
Vol 14 (1) ◽  
pp. 19 ◽  
Author(s):  
Zulkarnain Zulkarnain ◽  
Lizawati Lizawati
Keyword(s):  
Light Intensity ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Compact Structure ◽  
Plant Materials ◽  
Cotyledon Explants ◽  
Light Yellow ◽  
Materials Used ◽  
Embryogenic Callus Formation

The aim of this study was to develop an efficient method for the induction of embryogenic callus formation for in vitro propagation ofjatropha. Plant materials used were 30-days old in vitro seedlings, cut into hypocotyl and cotyledon (lower, middle and upper) sections.Medium used was MS composition supplemented with vitamins, 3% sucrose, 0.7% agar at pH 5.8 ± 1, and 2,4-D (0, 1, 2, 3, 4 dan5 mg l-1). Cultures were kept at temperature of 25 ± 1 0C with 50 μmol m-2 s-1 light intensity and 16-h photoperiod. The results indicated thatthe rate of callus formation depended on the source of explant, the application of 2,4-D, and the interaction of both. The fastest callusproliferation (2.33 days following initiation) was obtained on cotyledon explants cultured on medium without 2,4-D. The explant sourcesand 2,4-D concentrations also showed significant effect on the percentage of explant forming callus. The most callus formation (88.33%)was obtained on middle cotyledon cultured on 3 mg l-1 2,4-D, whereas the fewest (6.84%) was found on upper cotyledon cultured on mediumwithout 2,4-D. The colour of callus was dominated by white, light yellow, cream and brown with mostly compact structure, particularly onhypocotyl cultured on medium without 2,4-D. The texture of callus formed on hypocotyl treated with up to 4 mg l -1 2,4-D was dominatedby coarse appearance. In contrast, majority of callus proliferated on hypocotyl treated with 5 mg l -1 2,4-D or cotyledon treated with orwithout 2,4-D produced callus with smooth texture %.

scholarly journals Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene

2018 ◽  
Vol 22 ◽  
pp. 222-227
Author(s):  
O. M. Honcharuk ◽  
O. V. Dubrovna
Keyword(s):  
Triticum Aestivum ◽  
Transgenic Plants ◽  
Bread Wheat ◽  
Binary Vector ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Pcr Analysis ◽  
Genetic Construct ◽  
Agrobacterium Mediated Transformation

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.

scholarly journals Concentration of mineral elements in wheat (Triticum aestivum L.) straw: Genotypic differences and consequences for enzymatic saccharification

2015 ◽  
Vol 75 ◽  
pp. 134-141 ◽  
Author(s):  
Emiko Murozuka ◽  
Thomas C. de Bang ◽  
Jens Frydenvang ◽  
Jane Lindedam ◽  
Kristian H. Laursen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Enzymatic Saccharification ◽  
Triticum Aestivum L ◽  
Mineral Elements ◽  
Genotypic Differences
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