In vitro regeneration of the isolated shoot apical meristem of two commercial fig cultivars ‘Sabz’ and ‘Jaami-e-Kan’

2019 ◽  
Vol 17 ◽  
pp. 743-749
Author(s):  
Amir Sahraroo ◽  
Abdolkarim Zarei ◽  
Mesbah Babalar
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
In Vitro Regeneration

scholarly journals In vitro regeneration of pumpkin (cucurbita maxima) through shoot apical meristem

1970 ◽  
Vol 18 ◽  
pp. 104-107 ◽  
Author(s):  
ME Haque ◽  
D Rezwana ◽  
MA Islam ◽  
B Sikdar
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Total Yield ◽  
Meristem Culture ◽  
Culture Vessel ◽  
Cucurbita Maxima ◽  
Indirect Regeneration

Context: Pumpkin (Cucurbita maxima) is very important vegetable crop. It is damaged by several types of diseases, especially viral diseases which reduce the total yield of pumpkin. This is very difficult problem and such type of problem can be overcome by meristem culture. Objectives: To develop suitable protocols for indirect regeneration of pumpkin plant through shoot apical meristem.   Materials and Methods: Meristems were isolated from 15-21 days in vivo grown plants by collecting shoot tips and were prepared (0.2-0.5 mm) under 4X zoom stereo-microscope remaining two leaves primordia. Inoculation of explants was made singly per culture vessel in semisolid MS fortified with different concentrations and combinations of cytokinin and auxin.   Results: Among different concentrations, 1.0 mg/l BAP showed best response for callus induction. Calli were sub cultured for shoot formation in MS containing BAP singly or combination with GA3 and 1.5 mg/l BAP + 0.1 mg/l GA3 gave better result. In vitro regenerated shoots were rooted well in ½ strength MS with 0.5 mg/l IBA and micro plants were acclimatized successfully in natural condition.   Conclusion: Indirect regeneration of pumpkin plants through shoot apical meristem has been established.   Keywords: Growth regulators; in vitro regeneration; Pumpkin; Cucurbita maxima. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8784 JBS 2010; 18(0): 104-107

scholarly journals Regeneration of Plants from Apical Meristem Tips and Nodal Segments of Arachis pintoi

2003 ◽  
Vol 30 (2) ◽  
pp. 75-79 ◽  
Author(s):  
H. Y. Rey ◽  
L. A. Mroginski
Keyword(s):  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Regeneration Potential ◽  
Arachis Pintoi ◽  
Solid Media ◽  
One Step

Abstract The in vitro regeneration potential of shoot apical tips (2 to 3 mm in length), meristems (0.3 to 0.5 mm in length), and nodal segments (4 to 7 mm long with an axillary bud) of diploid (2n = 2x = 20) and triploid (2n = 3x = 30) cytotypes of Arachis pintoi was evaluated. Explants were cultured on MS medium supplemented with different concentrations and combinations of naphthaleneacetic acid (NAA) and benzyladenine (BA). In one experiment the effect of gibberellic acid was tested. The cultures were done in liquid and solid media. Plant regeneration can be readily achieved from all explants in one step of 30 d culture on MS + 0.01 mg/L each of NAA and BA or two steps consisting of 1) shoots regeneration through culture of explants on MS + 0.01 mg/L each of NAA and BA, and 2) induction of rooting in regenerated shoots by reculture on MS + 0.01 mg/L NAA. The plantlets were successfully transferred to pots in a greenhouse.

scholarly journals Phytohormones Influence on the In-Vitro Regeneration of Saccharum Officinarum L. from Apical Meristem Culture

2014 ◽  
Vol 5 (2) ◽  
pp. 85 ◽  
Author(s):  
Ejiroghene Felix Lawyer ◽  
Z. O. Jamaleddine ◽  
P. T. Lyam ◽  
I. T. Borokini ◽  
A. A. Adedeji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Saccharum Officinarum ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Indole Butyric Acid

Growth regulators especially auxins and cytokinins are critical for plant in-vitro regeneration. The effect of these plant growth regulators on in-vitro propagation of Saccharum officinarum L (Sugarcane) was investigated. In vitro response of two different varieties of sugarcane (NCS 005 and NCS 008) to Plant Growth Regulators was obtained in this study. Formation of buds was obtained on shoot apical meristem when cultured on MS (Murashige and Skoog) medium supplemented with 0.1mg/l BAP (6-Benzylaminopurine). After two weeks of initiation, regenerated meristem was inoculated into MS (Murashige and Skoog) fortified with different concentrations and combination of cytokinins. Shoot multiplication was optimal on 0.5mg/l BAP + 0.25 mg/l Kin(Kinetin) for NCS 005 variety while for NCS 008 variety, no significant (P≥0.05) difference was observed between 1.5mg/l BAP and 1.5mg/l BAP +0.5mg/l Kin. The best root induction for in vitro derived shoots was obtained on 1.0 mg/l NAA (Naphthalene acetic acid) and 2.0 mg/l IBA( Indole butyric acid) for both varieties of sugarcane within ten days of culture transfer. Successfully established plantlets showed excellent growth response when weaned under regulated green house conditions.

TWO TYPES OF ALBUGO-INDUCED GALLS ON IPOMOEA PENTAPHYLLA AND THEIR MORPHOGENETIC INTERPRETATION

1966 ◽  
Vol 44 (5) ◽  
pp. 535-540
Author(s):  
Hardev Singh ◽  
Krishna Bedi
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Rate Of Development ◽  
Distinct Morphology

Ipomoea pentaphylla Jacq. is a twining, hirsute annual. After infection by Albugo ipomoeae-panduranae (Schw.) Swingle, two types of galls develop on the stem—bud and cerebriform. The bud gall originates from an infected bud while the cerebriform gall arises from the internode. The two types are distinct from each other in their morphology, anatomy, rate of development, and morphogenesis. The bud gall is of the organoid type while the cerebriform gall is of the histioid type. Tissues of the bud gall grown under in vitro conditions give rise to a structure like the cerebriform gall. The very distinct morphology of the two types has been interpreted on the basis of the possible organizing influence of the shoot apical meristem.

Sign in / Sign up

Export Citation Format

Share Document