Establishment of a highly efficient regeneration system for in vitro culture of young wheat spikes

2006 ◽  
Vol 3 (2) ◽  
pp. 147-154
Author(s):  
Zhao Lin-Shu ◽  
Liu Lu-Xiang ◽  
Wang Jing ◽  
Zheng Qi-Cheng ◽  
Guo Hui-Jun ◽  
...  
Keyword(s):  
Callus Induction ◽  
Shoot Formation ◽  
Immature Embryos ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Regeneration System ◽  
Differentiation Medium ◽  
Efficient Regeneration ◽  
Young Spike

AbstractThis study used three winter wheat (Triticum aestivum L.) genotypes (H6756, H311 and SP8581) to compare the effects of sampling time, callus induction media, differentiation media and rooting media on in vitro culture of young spikes in wheat. In all these three genotypes, the frequencies of green plantlet differentiation were high when their young spikes were cultured between the stages of protective glume primordium formation and pistil and stamen primordium formation, but low at other stages. The optimum medium for callus induction was Murashige and Skoog (MS) medium+2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The optimum green plantlet differentiation medium was MS medium. Some abnormal plantlets regenerated from calli. When these plantlets were transferred to another differentiation medium [MS+1.0 mg/l 1-naphthaleneacetic acid (NAA)+0.2 mg/l 6-benzylaminopurine (6-BA)], shoot formation and elongation were induced. This allowed 90.91% of them to develop into normal green plantlets. The optimum rooting medium was 1/2MS+0.2 mg/l 3-Indolylacetonitrile (IAA)+80 g/l sucrose. An efficient regeneration system for young spike culture of wheat was set up based on such methods. Using this wheat-regeneration system, young spikes and immature embryos of 17 genotypes of wheat were in vitro cultured to study and compare the callus induction frequencies and green plantlet differentiation frequencies. The results of two successive years showed that in 15 out of the 17 genotypes (88.24%) the green plantlet differentiation frequencies were higher than those of immature embryos by 6.2–65.1%. These results showed that the regeneration system established in this trial for young spike culture of wheat was effective.

scholarly journals In vitro Propagation of Globba schomburgkii Hook. f. via Bulbil Explants

2017 ◽  
Vol 15 (10) ◽  
pp. 701-710
Author(s):  
Piyaporn SAENSOUK ◽  
Surapon SAENSOUK ◽  
Phattaraporn PIMMUEN
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Root Formation ◽  
Survival Rates ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Different Types

An efficient and rapid protocol for the micropropagation of Globba schomburgkii Hook. f. via bulbil explants was investigated. The long divided and undivided bubils of G. schomburgkii Hook. f. were cultured on MS medium (Murashige and Skoog) that had either 3 mg/l benzyladenine (BA) or 0.5 mg/l naphthaleneacetic acid (NAA) added for 8 weeks. The results indicated that the long divided bulbils of G. schomburgkii Hook. f. showed a greater amount of plant regeneration than the undivided bulbils. Callus induction, as well as shoot and root formation, were observed when culturing microshoots of 1 cm in length on media (MS) that had Thidiazuron (TDZ) or NAA plus BA added at a range of concentrations for 8 weeks. The highest percentage of callus induction was 40 % when culturing the microshoots on MS medium supplemented with NAA and BA. The best result for shoot formation was achieved when culturing the microshoots on MS medium with TDZ added. The highest number of roots was obtained when culturing the microshoots on MS medium with NAA and BA added. The in vitro-derived plantlets of G. schomburgkii Hook. f. were transplanted to pots containing different types of potting mixture in a greenhouse. The survival rates were 80 % when G. schomburgkii Hook. f. was transplanted to sand.

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

scholarly journals In vitro bulblet regeneration from immature embryos of endangered and endemic Muscari azureum

2011 ◽  
Vol 63 (1) ◽  
pp. 209-215 ◽  
Author(s):  
S. Uranbey
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Ornamental Plant ◽  
Immature Embryos ◽  
Induction Medium ◽  
Ms Medium ◽  
Culture Initiation ◽  
Mineral Salts ◽  
Font Color

A high frequency of bulblet regeneration was achieved for the endemic and endangered ornamental plant Muscari azureum using immature embryos. Immature embryos of M. azureum were cultured on a callus induction medium consisting of N6 mineral salts and vitamins, 400 gL-1 casein + 40 gL-1 sucrose + 2 mgL-1 L-proline, 2 mgL-1 2,4-D and 2 gL-1 Gelrite. Then the embryogenic callus clusters were transferred to a bulblet induction medium consisting of MS mineral salts and vitamins containing different concentrations and combinations of BAP, KIN, TDZ, Zeatin, IAA, NAA, 30 gL-1 sucrose and 7 gL-1 agar. Prolific bulblet multiplication (over 13 bulblets/embryo) was achieved from immature embryos after 5-6 months of culture initiation. Well-developed bulblets were excised and individually rooted on ? strength MS medium supplemented with 1 mgL-1 IBA, 0.5 gL-1activated charcoal, 20 gL-1sucrose and 6 gL-1agar and acclimatized. <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/ABS150608072E">10.2298/ABS150608072E</a><u></b></font>

scholarly journals In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

2010 ◽  
Vol 6 ◽  
pp. 103-105 ◽  
Author(s):  
Aditi Singh ◽  
Saroj K Sah ◽  
Aunji Pradhan ◽  
Sabari Rajbahak ◽  
Niran Maharajan
Keyword(s):  
Medicinal Plant ◽  
Callus Induction ◽  
Shoot Growth ◽  
Tinospora Cordifolia ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

Cytokinin-cytokinin interaction ameliorates the callus induction and plant regeneration of tomato (Solanum lycopersicum Mill.)

2012 ◽  
Vol 60 (1) ◽  
pp. 47-55 ◽  
Author(s):  
A. Ali ◽  
T. Yossef ◽  
A. El-Banna
Keyword(s):  
Plant Regeneration ◽  
Solanum Lycopersicum ◽  
Callus Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Benzyl Adenine ◽  
Cotyledon Explants ◽  
Tomato Genotypes ◽  
Number Of Shoots

The present study was carried out for developing an efficient in vitro callus induction and plant regeneration system in four different tomato genotypes (Solanum lycopersicum Mill., previous name: Lycopersicon esculentum), Advantage II, Edkawy, Castle Rock and Super Strain B, using hypocotyl and cotyledon explants. The effects of two cytokinins, BA (benzyl adenine) and Kin (kinetin), on callus induction and plant regeneration frequency were investigated when added to MS medium in combination at varying concentrations. All concentrations of the two cytokinins were suitable for callus induction and plant regeneration. The frequency of callus induction and plant regeneration from both cotyledon and hypocotyl explants reached 100% for all tested genotypes. Cotyledons produced a higher average number of shoots per explants than hypocotyls for all the genotypes in the five concentrations of combined cytokinins. The average number of shoots per explant in Super Strain B was found to be the highest (42 and 60 for the hypocotyl and cotyledon explants, respectively). Supplementing MS medium with 1.0 mg L−1 kinetin and 1.0 mg L−1 benzyl adenine was found to be optimum for producing the highest number of shoots per explant from hypocotyls and cotyledons in the tomato genotypes investigated. The proposed medium showed a significant superiority over the reference media.

scholarly journals Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

2021 ◽  
Vol 12 ◽  
Author(s):  
Denis Okello ◽  
Sungyu Yang ◽  
Richard Komakech ◽  
Yuseong Chung ◽  
Endang Rahmat ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Developmental Stages ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Regeneration Capacity ◽  
Root Explants ◽  
Indirect Regeneration

The medicinal plant, Aspilia africana, has been traditionally used in several African countries to treat many diseases such as tuberculosis, cough, inflammation, malaria, osteoporosis, and diabetes. In this study, we developed a protocol for in vitro propagation of A. africana using indirect shoot organogenesis from leaf and root explants of in vitro-grown seedlings and assessed the tissues at different developmental stages. The highest callus induction (91.9 ± 2.96%) from leaf explants was in the Murashige and Skoog (MS) medium augmented with 1.0 mg/L 6-Benzylaminopurine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) while from root explants, the highest callus induction (92.6 ± 2.80%) was in the same plant tissue culture medium augmented with 0.5 mg/L BAP and 1.0 mg/L 2,4-D. The best shoot regeneration capacity from leaf-derived calli (i.e., 80.0 ± 6.23% regeneration percentage and 12.0 ± 6.23 shoots per callus) was obtained in medium augmented with 1.0 mg/L BAP and 0.05 mg/L α-Naphthaleneacetic acid (NAA); the best regeneration capacity for root-derived calli (i.e., 86.7 ± 6.24% shoot regeneration percentage and 14.7 ± 1.11 shoots per callus) was obtained in the MS medium augmented with 1.0 mg/L BAP, 0.05 mg/L NAA, and 0.1 mg/L Thidiazuron (TDZ). Regenerated plantlets developed a robust root system in 1/2 MS medium augmented with 0.1 mg/L NAA and had a survival rate of 93.6% at acclimatization. The in vitro regenerated stem tissue was fully differentiated, while the young leaf tissue consisted of largely unorganized and poorly differentiated cells with large intercellular airspaces typical of in vitro leaf tissues. Our study established a protocol for the indirect regeneration of A. africana and offers a basis for its domestication, large-scale multiplication, and germplasm preservation. To the best of our knowledge, this is the first study to develop an indirect regeneration protocol for A. africana and conduct anatomical assessment through the different stages of development from callus to a fully developed plantlet.

scholarly journals Development of an Efficient Plant Regeneration System for the Selenium-hyperaccumulator Astragalus racemosus and the Nonaccumulator Astragalus canadensis

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 2138-2142 ◽  
Author(s):  
Chiu-Yueh Hung ◽  
Jiahua Xie
Keyword(s):  
Plant Regeneration ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Shoot Induction ◽  
Regeneration System ◽  
Rooting Medium ◽  
Significant Difference

A method of in vitro plant regeneration for both the selenium-hyperaccumulator Astragalus racemosus ‘Cream Milkvetch’ and the nonaccumulator Astragalus canadensis ‘Canadian Milkvetch’ was developed with two induction media, M1 and M2. The M1 and M2 contain Murashige and Skoog basal medium plus vitamins, 8.07 μm N-(2-chloro-4-pyridyl)-N′-phenylurea, 2.5% (w·v−1) sucrose, 0.7% (w·v−1) agar (pH 5.7), and 0.89 μm or 3.12 μm a-naphthaleneacetic acid, respectively. In vitro cultures were initiated on these two types of media with three types of explants: cotyledons, hypocotyls, and roots. More than 93% of cultured explants from both species could form calli or calli with shoots. With regard to shoot formation, A. canadensis could produce multiple shoots from all types of explants more efficiently than A. racemosus. The highest shoot induction was approximately three shoots per explant in A. racemosus, whereas A. canadensis could reach ≈10 shoots per explant. M1 could induce more shoots than M2 no matter what type of explant was used, but the overall induction rates were no significant difference. Among the three types of explants used, the cotyledons were the best explants for shoot induction in A. canadensis, whereas hypocotyls were the best in A. racemosus. In A. racemosus, shoots could also be obtained from calli on the rooting medium containing Murashige and Skoog basal plus vitamins, 2.84 μm indole-3 acetic acid, 2.5% (w·v−1) sucrose, and 0.7% (w·v−1) agar (pH 5.7). Approximately 43% of A. canadensis shoots and 19% of A. racemosus shoots could be rooted on the rooting medium.

In Vitro Regeneration of Valencia-type Peanut (Arachis hypogaea L.) from Cultured Petiolules, Epicotyl Sections and Other Seedling Explants1

1992 ◽  
Vol 19 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Ming Cheng ◽  
David C. H. Hsi ◽  
Gregory C. Phillips
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Arachis Hypogaea L ◽  
Wide Range ◽  
Cultivated Peanut

Abstract This study evaluated plant development via direct organogenesis from in vitro-cultured young seedling tissues of cultivated peanut, especially the valencia-type peanut. Complete plants were regenerated from in vitro-cultured petiolule-with-blade-attached explants, leaflet segments, and epicotyl andpetiole sections. Multiple shoots arose on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BA) (5–25 mg/L) plus 1-naphthaleneacetic acid (NAA) (0.5–3 mg/L). After 30 d culture on 25 mg/L BA + 1 mg/L NAA, 1.6 buds or shoots/explant were regenerated from the petiolule-with-blade-attached explants. Comparable numbers of shoots were obtained from epicotyl sections of the first node region of the seedling after 60 d culture using 10 mg/L BA + 1 mg/L NAA. Leaflet segments and petiole sections were less responsive for shoot formation. Excised shoots developed roots in vitro upon transfer for 15 d to MS medium supplemented with NAA at 1 mg/L. Plantlets were transferred to soil and grown in a greenhouse to maturity. A wide range of cultivated peanut genotypes was evaluated for organogenic responsiveness, using the petiolule-with-blade-attached explant source. Only valencia-type cultivars, or a hybrid derivative with a Valencia background, were responsive with this regeneration system.

Production of Glycyrrhizin in Callus Cultures of Licorice

2008 ◽  
Vol 63 (5-6) ◽  
pp. 413-417 ◽  
Author(s):  
Winida Wongwicha ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama ◽  
Indree Tuvshintogtokh ◽  
Waraporn Putalun
Keyword(s):  
Callus Induction ◽  
Shoot Formation ◽  
Callus Cultures ◽  
Glycyrrhiza Glabra ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
Shoot Induction

Licorice plants, Glycyrrhiza glabra, G. uralensis, and G. inflata, were investigated for callus induction using Murashige and Skoog (MS) medium combined with auxins and cytokinins. After 4 weeks of culture, 33-100% of leaf or stem explants formed calli. Maximum of shoot induction from callus cultures was achieved by G. inflata stem explants cultured on MS medium supplemented with 1 mg/l α-naphthaleneacetic acid (NAA) and 0.5 mg/l 6-benzyladenine (BA) (67%) which also gave maximum shoot formation per explant (two shoots per explant). These results indicated that all three Glycyrrhiza species regenerated shoots from callus cultures on MS medium combined with NAA and BA or only thidiazuron (TDZ; 0.1 and 0.5 mg/l). Glycyrrhizin contents of G. uralensis calli induced using MS medium in combination with NAA and BA [(27.60 ± 8.47) μg/g DW] or TDZ alone [(36.52 ± 2.45) μg/ g DW] were higher than those found in other combinations.

scholarly journals An efficient regeneration system through in vitro somatic embryogenesis of barley (Hordeum vulgare L.)

2019 ◽  
Vol 27 ◽  
pp. 89-99
Author(s):  
M Haque ◽  
SMS Islam
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Casein Hydrolysate ◽  
Ms Medium ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Hordeum Vulgare L ◽  
The Media ◽  
Barley Genotypes

This study was carried out to improve an efficient protocol for in vitro callus induction and plant regeneration using Bangladeshi barley genotypes collected from BARI, Gazipur, Bangladesh. After sterilization embryos were separated carefully from mature seeds of six barley genotypes (BB-1, BB-2, BB-3, BB-4, BB-5 and BB-6) and cultured them in MS medium supplemented with various concentration and combination of PGRs for callus induction and regeneration. Out of six genotypes BB-6 showed highest (38.17%) callus induction in MS + 4.0 mg/l 2,4-D + 200 mg/l L-proline + 300 mg/l casein hydrolysate; whereas, BB-4 and BB-5 showed no callus induction in the same medium. For plant regeneration from embryogenic calli the same genotype (BB-6) also performed the best results (19.25%) in MS medium supplemented with 1.5 mg/l BAP + 30 g/l sucrose. Analysis of variance (ANOVA) showed highly significant differences among the media and the genotypes. J. bio-sci. 27: 89-99, 2019

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