scholarly journals Application of agar-fill method to estimate compartment capacity of gastrointestinal tract in Syrian hamsters (Mesocricetus auretus)

1996 ◽  
Vol 76 (6) ◽  
pp. 833-840 ◽  
Author(s):  
Osamu Sasaki ◽  
Hisashi Shinohara ◽  
Toshihiro Yamagishi ◽  
Akira Nishida ◽  
Yutaka Mizuma

In the present study we have developed the agar-fill method for the measurement of gastrointestinal-tract capacity (GTC) to replace the in vitro water-fill method. This would estimate GTC without using complex equipment and techniques, and can be applied to the measurement of GTC for small laboratory animals. We attempted to confirm the efficiency of the agar-fill method by investigating the relationship between dietary neutral-detergent fibre (NDF) content and GTC. The digestion trials were carried out using the Syrian hamster (Mesocricefus auretus). The trials were conducted using both sexes, two age-groups and three levels of dietary NDF with a cross-classified design. The size of each gastrointestinal organ was determined as tissue weight(TW)and GTC. The DM intake, digestible DM intake, DM digestibility, NDF digestibfity, aciddetergent fibre(ADF) digestibfity and digesta transit time were also measured. GTC increased with increasing NDF content of the diets. TW responded similarly to increasing NDF content, but the response was smaller than that of GTC. DM digestibility decreased with increasing NDF content of the diet. The digestible DM intake did not decrease with increasing NDF because DM intake increased with NDF content. Digesta transit time was not shorter of the high-NDF-diet group but DM intake increased with increasing NDF content. NDF digestibility did not differ significantly between low- and medium-NDF diets. ADF digestibility was low in the low-NDF-diet group.The digestion characteristics were highly correlated with TW and GTC, except for TW of small intestine. These correlations were higher with GTC than with TW. The results of the present study confirm previous findings suggesting that the agar-fill method is a useful means of estimating GTC for small laboratory animals.

1977 ◽  
Vol 43 (5) ◽  
pp. 839-843 ◽  
Author(s):  
J. A. Severson ◽  
R. D. Fell ◽  
J. G. Tuig ◽  
D. R. Griffith

Plasma corticosterone concentrations and in vitro adrenal secretion of corticosterone were determined in exercise-trained rats. Rats, 100, 200, and 300 days of age, were trained for a 10-wk period by treadmill running. Following the training program, rats were subjected to an acute bout of swimming. Acute swimming elevated plasma corticosterone concentrations in all age groups. At 170 days of age, the plasma corticosterone concentration following swimming was higher in exercise-trained rats than in controls. The opposite was true of acutely swum rats at 270 and 370 days of age. Acute swimming elevated the in vitro adrenal gland response to adrenocorticotropic hormone stimulation in control rats at all ages and in trained rats at 170 days of age. The in vivo relationship of epinephrine and the pituitary adrenal system is suggested as a mechanism which could have caused this response. The relationship of secretion rates to plasma corticosterone concentrations indicated that extra-adrenal mechanisms, such as decreased turnover, were also responsible for the elevated plasma corticosterone levels observed in response to acute swimming.


2021 ◽  
Author(s):  
Yan Luo ◽  
Krystal Dozier ◽  
Carin Ikenberg

BACKGROUND An electronic personal health record (ePHR), also known as a personal health record (PHR), was broadly defined as an electronic application through which individuals can access, manage, and share their health information in a secure and confidential environment. Although ePHRs can benefit individuals as well as caregivers and healthcare providers, the use of ePHRs among individuals continues to remain low. The relationship between age and ePHRs use has been documented in previous studies, which indicated younger age was related to higher ePHRs use, and patients who are younger were more likely to use ePHRs. OBJECTIVE The current study aims to examine the relationship between human-technology interaction factors and ePHRs use among adults, and then compare the different effects of human-technology interaction factors on ePHRs use between younger adults (18-54 years old) and older adults (55 years of age and over). METHODS We analyzed the from the Health Information National Trends Survey (HINTS5, Cycle 3) collected from U.S. adults aged 18 years old and over in 2019. Descriptive analysis was conducted for all variables and each item of ePHRs use. Bivariate tests (Pearson test for categorical variable and F-test for continuous variables) were conducted over four age groups. Lastly, adjusting for socio-demographics and healthcare resources, a weighted multiple linear regression was conducted to examine the relationship between human-technology interaction factors and ePHRs use. RESULTS The final sample size was 1,363 and divided into two age groups: 18-54 years old and 55 years of age and older. The average level of ePHRs use was low (Mean=2.76, range=0-8). There is no significant difference on average ePHRs use between two age groups. Including clinical notes was positively related to ePHRs use in both groups: 18-54 years old (beta=0.28, P<0.01), 55 years old and above (beta=0.15, P<0.01). While accessing ePHRs using a smartphone app was only associated with ePHRs use among younger adults (beta=0.29, P<0.001), ease to understand health information in ePHRs was positively linked to ePHRs use only among older adults (beta=0.13, P<0.01). CONCLUSIONS This study found that including clinical notes was positively related to ePHRs use in both age groups, which suggested that including clinical notes as a part of ePHRs might improve the effective use of ePHRs among patients. Moreover, accessing ePHRs using a smartphone app was associated with higher ePHRs use among younger adults while ease of understanding health information in ePHRs was linked to higher ePHRs use among older adults. The design of ePHRs should provide the option of being accessible through mobile devices to promote greater ePHRs use among young people. For older adults, providers could add additional notes to explain health information recorded in the ePHRs.


Zygote ◽  
2006 ◽  
Vol 14 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Suha S. Kilani ◽  
Simon Cooke ◽  
Andrew K. Kan ◽  
Michael G. Chapman

Advanced female age and extended in vitro culture have both been implicated in zona pellucida (ZP) hardening and thickening. This study aimed to determine the influence of (i) the woman's age and (ii) prolonged in vitro culture of embryos on ZP thickness and density using non-invasive polarized light (LC-PolScope) microscopy. ZP thickness and density (measured as retardance) were determined in oocytes, embryos and blastocysts in women undergoing intracytoplasmic sperm injection (ICSI) in two age groups (older, >38 years; younger, ≤38 years). A total of 193 oocytes from 29 patients were studied. The younger group contained 100 oocytes and the older group 93 oocytes. The ZP was significantly thicker in metaphase II oocytes in the older group compared with the younger group (mean ± SD: 24.1 ± 2.5 μm vs 23.1 ± 3.3 μm; p = 0.01) but ZP density was equal (2.8 ± 0.7 nm). By day 2 of culture, embryos from the two groups had similar ZP thickness (22.2 ± 2.2 μm vs 21.7 ± 1.6 μm; p = 0.28) and density (2.9 ± 0.7 nm vs 2.8 ± 0.8 nm; p = 0.57). For the embryos cultured to blastocyst (older: n = 20; younger: n = 18) ZP thickness was similar in the two groups (19.2 ± 2.7 μm vs 19.1 ± 5.0 μm; p = 0.8) but thinner than on day 2. The older group had significantly denser ZP than the younger group (4.2 ± 0.5 nm vs 3.3 ± 1.0 nm, p < 0.01). Blastocysts from both groups had significantly denser ZP than their corresponding day 2 embryos (older: 4.2 ± 0.5 nm vs 2.9 ± 0.7 nm, p < 0.001; younger: 3.3 ± 1.0 nm vs 2.8 ± 0.8 nm, p = 0.013). It is concluded that there is little relationship between ZP thickness and its density as measured by polarized light microscopy. While ZP thickness decreases with extended embryo culturing, the density of the ZP increases. ZP density increases in both age groups with extended culture and, interestingly, more in embryos from older compared with younger women.


1993 ◽  
Vol 50 (11) ◽  
pp. 2421-2428 ◽  
Author(s):  
J. E. Paloheimo ◽  
Yong Chen

We present a method for estimating effective efforts or fishing mortality rates based on a linearized version of the catch equation. Catch-at-age for at least two age groups over a series of years is required. The method presupposes a value for natural mortality rate (M). The method is validated using simulated data with an appropriate error structure. The algorithm always converges to a set of effective efforts that are compatible with the known catches. Nevertheless, the solution to the basic equations is not unique although the different solutions are typically highly correlated. If the M assumed by the algorithm is the same as the actual M the iterated effective efforts are typically very close to the true effective efforts or fishing mortality rates. If the assumed M is too high or too low the pattern of effective efforts is still recovered to a high degree of accuracy, typically 0.90 < r < 1.00, even though M may be off by as much as 60%. When data for three or more age groups are available the method is extended to at least squares procedure that takes into account the increasing uncertainty of catches with age.


Author(s):  
Mariana Ing Malelak ◽  
Nathania Mirabel Halim

This study aims to examine spending patterns in the millennial generation in Surabaya. Respondents of this study were the millennial generation who were divided into two age groups, namely 21-28 years old, who were referred to as junior millennials, and those aged 29-36 years who were referred to as senior millennials. The analysis technique used is a crosstab to examine the relationship between age and spending pattern of the millennial generation and an independent-sample t-test to test the difference between the spending pattern of the junior millennials and senior millennials. This study showed a significant relationship between age and spending pattern on the millennial generation, and there is a significant difference between the spending pattern of the junior millennials and senior millennials.


2019 ◽  
Vol 5 (3) ◽  
pp. eaau0790 ◽  
Author(s):  
L. S. Hackl ◽  
A. R. Abizari ◽  
C. Speich ◽  
H. Zungbey-Garti ◽  
C. I. Cercamondi ◽  
...  

Iron deficiency and anemia are prominent contributors to the preventable disease burden worldwide. A substantial proportion of people with inadequate dietary iron rely on rice as a staple food, but fortification efforts are limited by low iron bioavailability. Furthermore, using high iron fortification dosages may not always be prudent in tropical regions. To identify alternative fortification formulations with enhanced absorption, we screened different iron compounds for their suitability as rice fortificants, measured in vitro gastric solubility, and assessed dietary iron bioavailability using stable isotopic labels in rural Ghanaian children. Isotopic incorporation in red blood cells indicates that in the two age groups of children investigated (4 to 6 and 7 to 10 years), formulations provided 36 and 51% of the median daily requirement in absorbed iron, respectively. We describe approaches to enhancing iron bioavailability from fortified rice, which can substantially contribute to the prevention of iron deficiency in rice-eating populations.


1987 ◽  
Vol 10 (1) ◽  
pp. 45-51 ◽  
Author(s):  
EvaJean Pickering ◽  
AvaJane Pickering ◽  
Mary L. Buchanan

One aspect of social perception often noted as deficient in learning disabled individuals is an appropriate sense of humor. The purpose of this study was to examine the cartoon humor comprehension of learning disabled and non-handicapped boys in two age groups, 8- and 12-year-olds. The cognitive structure rather than the content of humor was the focus. In addition to examining comprehension of cartoon humor between groups and across age levels, the relationship between comprehension and appreciation measures was studied. Significant main effects were found indicating a developmental lag for the learning disabled subjects. Moderate correlations were established between comprehension and appreciation measures.


2021 ◽  
pp. 1-6
Author(s):  
Maxime Maheu ◽  
Mujda Nooristani ◽  
Brahim Kaci ◽  
Karina Moïn-Darbari ◽  
Benoit-Antoine Bacon ◽  
...  

BACKGROUND: Cervical Vestibular Evoked Myogenic Potentials (cVEMP) is an electromyogenic measure commonly used in clinic to assess saccule function. The main parameters are peak-peak amplitude and interaural asymmetry ratio (IAR). Several non-vestibular factors may influence these parameters. Notably, a greater EMG contraction level prior to stimulation leads to an increased amplitude. As aging impacts both vestibular structures and muscle propreties, it is still a matter of debate whether the decrease in cVEMP amplitude observed in normal aging is due to EMG differences prior to stimulation or to the effect of aging on the sacculo-collic reflex pathway. At the clinical level, understanding the effect of aging on the relationship between EMG activity and cVEMP response (amplitude, asymmetry ratio) and the effect of normalization is crucial to improving the categorization of healthy versus pathological responses. OBJECTIVE: To investigate whether normalization modifies cVEMP amplitude and asymmetry ratios differently in younger and older heatlhy adults. METHOD: cVEMP recordings were conducted in 42 normal healthy participants divided in two age groups: younger (n = 29): mean = 22.79 years old SD = 1.66; and older (n = 13): mean = 69.00 years old SD = 3.61. Air-conducted cVEMP were recorded using Eclipse (Interacoustics, Denmark). The stimulus was a 95 dBnHL tone burst (500 Hz) with rise, plateau and fall time of 1 ms. cVEMP were recorded only when EMG levels were between 50μV and 150μV, using the Eclipse (Interacoustic, Denmark) monitoring system. RESULTS: No significant differences were observed for prestimulus EMG levels between younger and older participants (F(1,83) = 1.13, p = 0.291). However, significant differences between groups were observed for raw cVEMP amplitude (F(1,83) = 14.78; p <  0.001) and corrected cVEMP amplitude (F(1,83) = 21.85; p <  0.0001). A significant positive linear relationship between prestimulus EMG contraction level (RMS) and raw cVEMP amplitude was observed in younger participants (r2 = 0.234; p <  0.001), but not in older adults (r2 = –0.0144; p = 0.056). Finally, no significant differences between younger and older participants were observed for raw amplitude asymmetry ratios (F(1,41) = 0.124, p = 0.726) or normalized asymmetry ratios (F(1,41) = 0.726, p = 0.508). CONCLUSION: Our results suggest that when EMG is monitored and activation of the SCM is sufficient, the observed decline in cVEMP amplitude with normal aging does not seem to be caused by EMG differences and is therefore likely due to the known histopathological modifications of the vestibular system that occurs with normal aging.


2016 ◽  
Vol 61 (2) ◽  
pp. 62-72
Author(s):  
Małgorzata Podogrodzka

In the assessment of the demographic age and ageing a lot of measures are being exploited. Among the most commonly used may be mentioned those which express the relationship between the number of people in two age groups (the elderly and the general population or younger age group). Rarely are used such measures, which take into account all the age structure of the population. The article presents a new method to analyze spatial diversity of the population age structure using the method of agglomeration with the Euclidean metric. The test objects were the voivodships (provinces), and the explanatory variables were five-year age groups (20 variables). In addition, a hypothetical business was introduced, which has a regressive age structure. Its introduction allowed for an evaluation of how the test regions differ not only among themselves, but also how far they deviate from the pattern of development of the Polish population. Considerations were conducted separately for men and women and the 1991, 2001, 2011.


Author(s):  
Aldo M. Costa ◽  
Alexandra Frias ◽  
Sandra S. Ferreira ◽  
Mario J. Costa ◽  
António J. Silva ◽  
...  

This study aimed to analyze the relationship between perceived aquatic competence (PAC) and real aquatic competence (RAC) in 6 to 10 year old children in skills identified as relevant for surviving an aquatic accident. The study sample consisted of 105 children (8.2 + 1.3 years old). Two age groups were examined separately [G1, 6 to 7 years (n = 53); G2, 8 to 10 years (n = 52)]. Children’s aquatic competence was evaluated twice for skills linked to the risk of drowning: Firstly, using a common swimsuit (simple condition) and secondly wearing a t-shirt (complex condition). The PAC was assessed by questionnaire interview. Pearson correlation coefficients, pairwise, and independent t-test comparisons were performed with a significance level of 5%. Similar levels of PAC were found among both age groups for all measured skills, excepted for breath control during swimming (p < 0.05). However, PAC differs significantly (p < 0.001) from RAC (in simple and complex conditions) only in G1. Correlations between PAC and RAC were modest for all measured skills in both age-groups. Significant differences were found between RAC in simple and complex conditions in both groups (p < 0.01). Age contributes to a higher perceived aquatic competence in skills related to the risk of drowning.


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